A 198.8 m deep borehole was drilled through ice to subglacial bedrock in the northwestern marginal part of Princess Elizabeth Land, ~12 km south of Zhongshan Station, in January–February 2019. Three years later, in February 2022, the borehole temperature profile was measured, and the geothermal heat flow (GHF) was estimated using a 1-D time-dependent energy-balance equation. For a depth corresponding to the base of the ice sheet, the GHF was calculated as 72.6 ± 2.3 mW m−2 and temperature −4.53 ± 0.27°C. The regional averages estimated for this area based, generally, on tectonic setting vary from 55 to 66 mW m−2. A higher GHF is interpreted to originate mostly from the occurrence of metamorphic complexes intruded by heat-producing elements in the subglacial bedrock below the drill site.

Very few studies have emphasized the effects of high-pressure sintering on snow density evolution, even though snow as a type of engineering material is widely used in construction engineering in cold regions for snow pavement, snow runway and polar infrastructure. This study presents new experimental results of snow densification under high pressures of up to 100 MPa for a temperature range from −3.5 to −17.3°C and uniaxial compression at the temperature of −10°C and constant strain rates from 5 × 10−4 to 10−1 s−1. Results reveal that density evolution of snow to ice under high-pressure sintering can be achieved in a wide temperature range within a duration as short as 5 min. The compressive strength of snow-sintered ice was ~1.2–2.2 times as large as that of water-frozen ice reported by previous work. The orthogonal experiment showed that pressure is a more significant factor affecting the final density in comparison with sintering temperature and time. The increased rates of ice fabrication, low limitations on temperature and reliable sintered snow strength indicate that snow-ice engineering, such as airport construction in Greenland and Antarctica, can be improved by high-pressure sintering of snow to overcome the harsh environment.

Hot-point drills have been widely used for drilling boreholes in glaciers, ice caps and ice sheets. A hot-point drill melts ice through the thermal head at its bottom end. Penetration occurs through a close-contact melting (CCM) process, in which the ice is melted, and the meltwater is squeezed out by the exerted force applied on the thermal head. During the drilling, a thin water film is formed to separate the thermal head from the surrounding ice. For the hot-point drill, the rate of penetration (ROP) is influenced by several variables, such as thermal head shape, buoyancy corrected force (BCF), thermal head power (or temperature) and ice temperature. In this study, we developed a model to describe the CCM process, where a constant power or temperature on the working surface of a thermal head is assumed. The model was developed using COMSOL Multiphysics 5.3a software to evaluate the effects of different variables on the CCM process. It was discovered that the effect of thermal head shape and the cone angle of conical thermal head on ROP is less significant, whereas the increase in the BCF and the power (or temperature) of the thermal head can continuously enhance the ROP.

Drilling and sampling are the most direct and effective methods available to study Antarctic subglacial lakes. Based on the Philberth probe, a Recoverable Autonomous Sonde (RECAS) allows for in situ lake water measurement and sampling, through the addition of an upper thermal tip and a cable recoiling mechanism. RECAS-200, a prototype of RECAS, has a drilling depth of 200 m, a surface supply voltage of 800 VAC and a downhole power of ~9.6 kW during drilling. In this study, a heating control system for RECAS-200 was designed. The system avoids the need for high-power step-down converters, by separating heating power from control power, thereby reducing the overall weight of the probe and avoiding the need to increase cable diameter. We also introduce a self-developed, small, solid-state, 800 VAC power regulator and a fuzzy PID temperature control algorithm. Their purpose was to manage the power adjustment of each heating element and to provide closed-loop temperature control of certain heating elements which can easily burn out due to overheating. Test results indicated that the proposed RECAS-200 heating control system met all our design specifications and could be easily assembled into the RECAS-200 probe.

In glaciology, snow–firn temperature at 10 m is considered a representation of the mean annual air temperature at the surface (MAAT) of the studied site. Although MAAT is an important parameter in ice-sheet investigations, it has not been widely measured in Antarctica. To measure the 10 m snow–firn temperature in Antarctica, a shallow hot-point drill system is designed. In this simple and lightweight system, a hot-point drill can melt boreholes with a diameter of 34 mm in the snow–firn to a depth of 30 m and a temperature sensors string can measure the borehole temperature precisely. In the 2018/19 field season, 16 boreholes along the Zhongshan–Dome A traverse were drilled, and the borehole temperature was measured. Although certain problems existed pertaining to the hot-point drill, a total depth of ~244 m was successfully drilled at an average penetration rate of ~10 m h−1. After borehole drilling, ~12–15 h were generally required for the borehole to achieve thermal equilibrium with the surroundings. Preliminary results demonstrated that the 10 m snow–firn temperature along the traverse route was affected by the increasing altitude and latitude, and it decreased gradually with an increase in the distance from Zhongshan station.

A series of new synthetic armored cables were developed and tested to ensure that they were suitable for use with the RECoverable Autonomous Sonde (RECAS), which is a newly designed freezing-in thermal ice probe. The final version of the cable consists of two concentric conductors that can be used as the power and signal lines. Two polyfluoroalkoxy jackets are used for electrical insulation (one for insulation between conductors, and the other for insulation of the outer conductor). The outer insulation layer is coated by polyurethane jacket to seal the connections between the cable and electrical units. The 0.65 mm thick strength member is made from aramid fibers woven together. To hold these aramid fibers in place, a sheathing layer was produced from a polyamide fabric cover net. The outer diameter of the final version of the cable is ~6.1 mm. The permissible bending radius is as low as 17–20 mm. The maximal breaking force under straight tension is ~12.2 kN. The cable weight is only ~0.061 kg m−1. The mechanical and electrical properties and environmental suitability of the cable were determined through laboratory testing and joint testing with the probe.

Hot-water ice-coring drills are often used to recover ice core samples from desirable depths in conjunction with full-scale hot-water drilling systems. However, the recovered cores exhibit varying qualities. The coring performance of a hot-water ice-coring drill depends significantly on the structure of the coring drill head (nozzle angle, diameter and number). To discover the most significant factor affecting ice-coring performance, nine types of drill heads were designed and tested in this study according to the orthogonal test design. Results indicated that the nozzle angle is the most significant factor that affects the coring quality and the optimal angle is ~15°. The number of nozzles is the second most important factor; a large number assists in obtaining ice cores of high quality. The optimal nozzle configuration to recover good quality cores are the following: the nozzle diameter, number of nozzles and nozzle angle are 1 mm, 60 nozzles and 15°, respectively, with the maximum diameter and 2 mm, 60 nozzles and 15°, respectively, with the maximum length.

The Antarctic subglacial drilling rig (ASDR) is designed to recover 105 mm-diameter ice cores up to 1400 m depth and 41.5 mm-diameter bedrock cores up to 2 m in length. In order to ensure safe and convenient drilling, drilling auxiliaries are designed to support fieldwork and servicing. These auxiliaries are subdivided into several systems for power supply, drill tripping in the borehole, ice core and chip processing, and drill servicing and maintenance. The required equipment also includes two generators, a drilling winch with a cable, logging winch with a cable, control desk, pipe handler with a fixed clamp, chip chamber vibrator, centrifuge, emergency devices and fitting and electrical tools. Additionally, several environmental protective measures such as a new liquid-tight casing with a thermal casing shoe and a bailing device for recovering drilling fluid from the borehole were designed. Most of the auxiliaries were tested during the summer of 2018–2019 near Zhongshan Station, East Antarctica while drilling to the bedrock to a depth of 198 m.

A new, modified version of the cable-suspended Ice and Bedrock Electromechanical Drill (IBED) was designed for drilling in firn, ice, debris-rich ice and rock. The upper part of the drill is almost the same for all drill variants and comprises four sections: cable termination, a slip-ring section, an antitorque system and an electronic pressure chamber. The lower part of the IBED comprises an auger core barrel, reamers, a core barrel for ice/debris-ice drilling and a conventional geological single-tube core barrel or custom-made double-tube core barrel. First, the short and full-scale field versions of the IBED were tested at an outdoor testing stand and a testing facility with a 12.5 m-deep ice well. Then, in the 2018–2019 summer season, the IBED was tested in the field at a site ~12 km south of Zhongshan Station, East Antarctica, and a ~6 cm bedrock core was recovered from a 198 m-deep borehole. A total of 18 d was required to penetrate the ice sheet. The retrieved core samples of blue ice, basal ice and bedrock provided valuable information regarding the Earth's paleo-environment.

Subacute ruminal acidosis (SARA) can increase the level of inflammation and induce rumenitis in dairy cows. Berberine (BBR) is the major active component of Rhizoma Coptidis, which is a type of Chinese anti-inflammatory drug for gastrointestinal diseases. The purpose of this study was to investigate the anti-inflammatory effects of BBR on lipopolysaccharide (LPS)-stimulated rumen epithelial cells (REC) and the underlying molecular mechanisms. REC were cultured and stimulated with LPS in the presence or absence of different concentrations of BBR. The results showed that cell viability was not affected by BBR. Moreover, BBR markedly decreased the concentrations and mRNA expression of pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin-1β, and interleukin-6 in the LPS-treated REC in a dose-dependent manner. Importantly, Western blotting analysis showed that BBR significantly suppressed the protein expression of toll-like receptor 4 (TLR4) and myeloid differentiation primary response protein (MyD88) and the phosphorylation of nuclear factor-κB (NF-κB), inhibitory kappa B (IκBα), p38 mitogen-activated protein kinase (MAPK), and c-Jun N-terminal kinase (JNK) in LPS-treated REC. Furthermore, the results of immunocytofluorescence showed that BBR significantly inhibited the nuclear translocation of NF-κB p65 induced by LPS treatment. In conclusion, the protective effects of BBR on LPS-induced inflammatory responses in REC may be due to its ability to suppress the TLR4-mediated NF-κB and MAPK signaling pathways. These findings suggest that BBR can be used as an anti-inflammatory drug to treat inflammation induced by SARA.

The Chinese black honey bee is a distinct honey bee subspecies distributed in the Xinjiang, Heilongjiang and Jilin Provinces of China. We conducted a study to investigate the genetic origin and the parasite/pathogen profile on Chinese black honeybees. The phylogenetic analysis indicated that Chinese black honeybees were two distinct groups: one group of bees formed a distinct clade that was most similar to Apis mellifera mellifera and the other group was a hybrid of the subspecies, Apis mellifera carnica, Apis mellifera anatolica and Apis mellifera caucasica. This suggests that the beekeeping practices might have promoted gene flow between different subspecies. Screening for pathogens and parasites showed that Varroa destructor and viruses were detected at low prevalence in Chinese black honeybees, compared with Italian bees. Further, a population of pure breeding black honeybees, A. m. mellifera, displayed a high degree of resistance to Varroa. No Varroa mites or Deformed wing virus could be detected in any examined bee colonies. This finding suggests that a population of pure breeding Chinese black honeybees possess some natural resistance to Varroa and indicated the need or importance for the conservation of the black honeybees in China.