This study aims to evaluate ship collision frequency in port fairways. One case study is created using one month's real-time ship movement data from five major Singapore port fairways. Results show that tankers account for the biggest proportion in the Temasek fairway, whereas the percentage of Roll-on-Roll-Off (RORO) and passenger ships is quite small in the Temasek fairway, Sinki fairway, Jong fairway and Southern fairway. Tankers and container ships are the two major ship types involved in dangerous encounters. The largest number of dangerous head-on and overtaking encounters is located in the Jong fairway. The majority of dangerous crossing encounters have occurred in the West Keppel fairway and Jong fairway. Ship collision frequency at night is found to be significantly higher than during the day in these fairways.