To lobby legislators, it is important for interest groups to signal their ability to help legislators win elections and provide them with policy-relevant information. We explore for-profit companies’ use of environmental, social, and governance (ESG) reports as a signaling device to promote their reputation to legislators and convey their ability to provide electoral and policymaking support, which is valuable for lobbying. To this end, we create a panel dataset by combining ESG reports issued by US companies and the same companies’ lobbying and campaign contribution records from 1999 to 2017. We expect companies to issue more ESG reports, as well as reports containing more quantitative content, when they lobby. The data conform to our expectations. We also reason that lobbying may be more strongly related to ESG reporting when it is coupled with campaign contributions made by affiliated corporate political action committees, but the data do not support this expectation.

When a droplet coalesces with a flat liquid–air interface, a secondary drop may be left behind resulting in only a partial coalescence rather than complete coalescence. In this paper, we employ an arbitrary Lagrangian–Eulerian method to demonstrate that applying an electric field favours the occurrence of partial coalescence. To understand this phenomenon, we systematically study the effect of an external electric field on the coalescence process between a droplet and a liquid–air interface. In an electric field, the induced electric stresses can overcome the downward flow of the droplet, thus lifting it upwards. As a result, the positive Laplace pressure in the neck region squeezes the droplet towards pinch-off. We observe that both the initial neck expansion and neck shrinkage are suppressed by the electric field. These effects become weaker as the Ohnesorge number $Oh$ increases. Based on the scaling analysis, we report a critical Ohnesorge number $O{h_c} = 14.39{\varGamma ^{3/2}} + 0.029$ to quantify the transition from partial coalescence to complete coalescence in the presence of an electric field, where $\varGamma $ represents the dimensionless electric Bond number. Finally, a relationship between the secondary droplet size and the two key dimensionless numbers of $Oh$ and $\varGamma $ has been developed, which could be useful for producing droplets of desired sizes in microfluidic applications.

We extract dynamic conditional factor premiums from the Fama-French factor model and find that most anomalies disappear after one accounts for time variation in these premiums. Vector autoregression evidence shows that mutual causation between dynamic conditional alphas and macroeconomic surprises serves as a core qualifying condition for fundamental factor selection. This economic insight is an incremental step toward drawing a distinction between rational risk and behavioral mispricing models. To the extent that dynamic conditional alphas can reveal the marginal investor’s fundamental news and expectations about the cross-section of average asset returns, our economic insight helps enrich macroeconomic asset return prediction.

Pumilio3 (Pum3), an evolutionarily distant homologue of the classical RNA-binding protein PUF (PUMILIO and FBF) family member, is also involved in the process of RNA metabolism through post-transcriptional regulation. However, the functions of Pum3 in mouse oocyte maturation and preimplantation embryonic development have not been elucidated. By comparing RNA levels in different tissues, we found that Pum3 was widely expressed in multiple tissues, but moderately predominant in the ovary. Histochemical staining suggested that the PUM3 protein exhibits positive signals in oocytes, granulosa cells and theca cells of different follicle stages. Oocyte immunofluorescence results showed a slightly higher level of PUM3 protein in metaphase II compared with the germinal vesicle (GV) stage. After knockdown of Pum3 in GV oocytes using siRNA injection (siPUM3), no obvious defect was observed in the processes of GV breakdown and polar body extrusion during in vitro maturation (IVM) for the siPum3 oocytes. Compared with the control group, the siPUM3 group displayed no significant abnormality in the cleavage and blastocyst formation rate of these fertilized oocytes. Therefore, we can conclude that depletion of Pum3 does not affect mouse oocyte maturation and early embryonic development in vitro.

The idea of translanguaging has disrupted much of the thinking in bilingual education. A common misunderstanding, however, is that translanguaging was intended to be a language teaching strategy. This article seeks to explore what a translanguaging approach to language teaching entails, with specific reference to the education of minoritized and racialized bilingual and multilingual learners in the school systems in English-dominant countries such as the UK. In particular, I highlight the connections with and contributions to the inclusion and social justice agenda that the translanguaging project aims to make. Translanguaging takes one step further from multilingualism in challenging the raciolinguistic ideologies that view bilingual learners as having separate languages and languaging lives. It instead views their racial/ethnic identities and linguistic practices together, that is, their translanguaging being. My main argument here is that to use translanguaging as a pedagogy for inclusion and social justice requires a change of mindset, not just practice – that is, translanguaging pedagogy rather than pedagogical translanguaging – which can be achieved through processes of ‘co-learning’ and ‘transpositioning’.

Flow around a tethered model of a swimming batoid fish is studied by using the wall-modelled large-eddy simulation in conjunction with the immersed boundary method. A Reynolds number ($Re$) up to 148 000 is chosen, and it is comparable to that of a medium-sized aquatic animal in cruising swimming state. At such a high $Re$, we provide, to the best of our knowledge, the first evidence of hairpin vortical (HV) structures near the body surface using three-dimensional high-fidelity flow field data. It is observed that such small-scale vortical structures are mainly formed through two mechanisms: the leading-edge vortex (LEV)–secondary filament–HV and LEV–HV transformations in different regions. The HVs create strong fluctuations in the pressure distribution and frequency spectrum. Simulations are also conducted at $Re=1480$ and 14 800 to reveal the effect of Reynolds number. Variations of the flow separation behaviour and local pressure with $Re$ are presented. Our results indicate that low-$Re$ simulations are meaningful when the focus is on the force variation tendency, whereas high-$Re$ simulations are needed when concerning flow fluctuations and turbulence mechanisms.

This paper presents a robust train localisation system by fusing a Global Navigation Satellite System (GNSS) with an Inertial Navigation System (INS) in a tightly-coupled (TC) strategy. To improve navigation performance in GNSS partly blocked areas, an advanced map-matching (MM) measurement-augmented TC GNSS/INS method is proposed via an error-state unscented Kalman filter (UKF). The advanced MM generates a matched position using a one-step predicted position from a UKF time update step with binary search algorithm and a point–line projection algorithm. The matched position inputs as an additional measurement to fuse with the INS position to augment the degraded GNSS pseudorange measurement to optimise the state estimation in the UKF measurement update step. Both the real train test on the Qinghai–Tibet railway and the simulation were carried out and the results confirm that the proposed advanced MM measurement-augmented TC GNSS/INS with error-state UKF provides the best horizontal positioning accuracy of 0 ⋅ 67 m, which performs an improvement of about 71% and 90% with respect to TC GNSS/INS with only error-state UKF and only error-state Extended Kalman filter in GNSS partly blocked areas.

Currently there is lack of knowledge on how new types of alternative fuels and their storage conditions change the droplet evaporation characteristics. Liquid fuel is commonly stored in wide varieties of containers, where fuel characteristics may change because of the exposure to the material of the container. This study evaluates the impact of different storage containers on droplet evaporation characteristics of different fuels. It was found that there is a distinct phase transition between high volatility to low volatility phase in each fuel stored in steel drums verses fuel that is stored in plastic drums. The type of fuel contaminated by polymer additive has a high impact on fuel droplet burn rates. Polymer additives also have an impact on nucleate boiling, sub-droplets and soot particles. The burning rate constant, K, of selected pure aromatics, various fuel mixtures and Jet A with different cetane numbers have been evaluated. Research has shown that in the low volatility combustion phase a trend reduction of lowest boiling point pure aromatic burning rate to the highest boiling point pure aromatic burning rate is obvious. Irregular change in droplet diameter between the high volatility phase and low volatility phase during the combustion of aromatics blend was observed. This work has also evaluated the relationship between burning rates and cetane numbers.

The subduction of the Bangong–Nujiang Ocean is important in the geological evolution of the Tibetan Plateau. In this paper, we report new zircon U-Pb age and Lu-Hf isotopic data and whole-rock elemental and Sr-Nd-Pb isotopic data for Early Cretaceous dacites from the Rena-Co area (RCA) in the southern Qiangtang Terrane (QT), central Tibet and use these data to better understand the tectonic evolution of the Bangong–Nujiang suture. LA–ICP-MS dating of zircons yields ages of 109.5 ± 0.6 Ma to 109.6 ± 0.8 Ma for the dacites from the RCA. Geochemically, these dacites are medium-K calc-alkaline and show high SiO2 contents of 64.79–70.37 wt.%, high Sr contents of 517–598 ppm and low Y contents of 8.45–10.7 ppm, similar to those of typical adakites. Additionally, all the rocks are strongly enriched in light rare earth elements and some large ion lithophile elements (e.g. Rb, U, K and Cs) but significantly depleted in high-field-strength elements (e.g. Nb, Ta and Ti), consistent with the geochemical characteristics of arc-type magmas formed in the subduction zone. Moreover, these adakite-like dacites show whole-rock initial (87Sr/86Sr)i ratios of 0.705119 to 0.705491, (206Pb/204Pb)i ratios of 18.489 to 18.508, (207Pb/204Pb)i ratios of 15.591 to 15.612, (208Pb/204Pb)i ratios of 38.599 to 38.686, ϵNd(t) values of −0.28 to +1.25 and single-stage Nd model ages of 642 to 818 Ma, as well as significantly positive zircon ϵHf(t) values of 3.9–13.1, with young Hf-depleted mantle ages of 331 to 923 Ma. These geochemical and isotopic data indicate that they are most likely derived from the juvenile thickened mafic lower continental crust, which contains partial melts of metasomatized peridotite and subduction-related fluids in the magma source region. Based on previous studies and our new data, we propose that the RCA adakite-like dacites are most likely a result of the northwards subduction of the Bangong–Nujiang Ocean lithosphere beneath the southern QT during the Early Cretaceous and that a slab rollback model could explain the formation of the RCA adakite-like dacites.

OBJECTIVES/GOALS: Glioblastoma (GBM) is the most common and aggressive adult primary brain malignancy. Clinically, GBM is refractory to T cell immune checkpoint blockade (ICB), in part due to its dense immune suppressive myeloid stroma. Here we show that myeloid-targeting STING agonists can repolarize the GBM microenvironment to cure ICB-refractory GBM models. METHODS/STUDY POPULATION: Using the synthetic cyclic di-nucleotide STING agonist IACS-8803 (8803) we treated orthotopic ICB-refractory QPP8 orthotopic murine GBM tumors intratumorally. We then analyzed survival and performed high parameter flow cytometry profiling of the tumor immune microenvironment following STING agonist treatment. To assess the contribution of adaptive immunity to STING agonist therapeutic efficacy, we treated orthotopic QPP8 tumors implanted in RAG1 KO mice and monitored survival. RESULTS/ANTICIPATED RESULTS: We found that STING agonist therapy cured murine orthotopic QPP8 tumors, in contrast to ICB that showed no survival benefit. In RAG1-/- mice bearing QPP8 tumors STING agonist therapy extended survival, however, the curative effect observed in wild-type mice was lost in the absence of adaptive immunity. STING agonist-treated QPP8 tumors displayed increased counts of CD8 T cells and NK cells, and decreased CD8 T cell PD1 expression. Infiltration of STING-treated gliomas by Ly6C+ F4/80+ Mono-MDSC substantially increased; however, these cells expressed reduced CD206 and CD163, suggestive of reduced immuno-suppression. Finally, in the cervical LN of QPP8-treated mice the frequency and CD80/CD86 expression of cDC1 cells increased.​ DISCUSSION/SIGNIFICANCE: ICB has failed in GBM, and the suppressive myeloid stroma remains a major barrier to generating anti-GBM T cell responses. Our work shows that STING activation, which primarily targets innate immunity myeloid cells 'upstream' of T cells in the antitumor immunity cycle, can cure ICB-refractory GBM tumors in an adaptive immunity-dependent manner.