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Ovarian volume decreases significantly in each 10-year period of a woman's fertile life. The ovarian size decreases in women greater than 40 years old. The volume of each ovary is calculated by measuring in three perpendicular directions and applying the formula for an ellipsoid. Using the largest cross-sectional sagittal view of the ovary, the mean ovarian diameter could be calculated from measurement of two perpendicular diameters. The combination of transvaginal ultrasound and pulsed color Doppler is increasingly used in gynecology to assess the hemodynamic changes in various physiological and pathological situations of the pelvic organs. Only one study has compared the predictive value of antral follicle count (AFC) measurement made using both two-dimension and three-dimension ultrasound in determining the outcome of response to ovarian stimulation as measured by the number of follicles that develop, the number of oocytes retrieved, and the pregnancy rate following assisted reproductive technologies (ART).