The geology of the Darwin Forest in central-western Argentina has been extensively studied since it was originally described by the author of On the Origin of Species. However, its role as a source and quarry of lithic raw materials throughout human history in the region has received less attention. In this paper, we evaluate the potential of the fossil trunks as knappable resources. Geological and archaeological surveys characterize the different features of human use in the Paramillos and Los Colorados geological formations. Petrographics and GIS analysis are also developed. Results show a strategic location of the sources related to Uspallata valley and Precordilleran sites (Agua de la Cueva). The favorable conditions related to accessibility and visibility, as well as quality and abundance of these raw materials explain the extensive exploitation demonstrated in quarried trunks and the corresponding incidence of primary debitage in surface areas.