Long-duration gamma-ray burst (GRB) afterglow observations offer cutting-edge opportunities to characterise the star formation history of the Universe back to the epoch of reionisation, and to measure the chemical composition of interstellar and intergalactic gas through absorption spectroscopy. The main barrier to progress is the low efficiency in rapidly and confidently identifying which bursts are high redshift ( $z > 5$ ) candidates before they fade, as this requires low-latency follow-up observations at near-infrared wavelengths (or longer) to determine a reliable photometric redshift estimate. Since no current or planned gamma-ray observatories carry near-infrared telescopes on-board, complementary facilities are needed. So far this task has been performed by instruments on the ground, but sky visibility and weather constraints limit the number of GRB targets that can be observed and the speed at which follow-up is possible. In this work we develop a Monte Carlo simulation framework to investigate an alternative approach based on the use of a rapid-response near-infrared nano-satellite, capable of simultaneous imaging in four bands from $0.8$ to $1.7\,\unicode{x03BC}$ m (a mission concept called SkyHopper). Using as reference a sample of 88 afterglows observed with the GROND instrument on the MPG/ESO telescope, we find that such a nano-satellite is capable of detecting in the H-band (1.6 $\unicode{x03BC}$ m) $72.5\% \pm 3.1\%$ of GRBs concurrently observable with the Swift satellite via its UVOT instrument (and $44.1\% \pm 12.3\%$ of high redshift ( $z>5$ ) GRBs) within 60 min of the GRB prompt emission. This corresponds to detecting ${\sim}55$ GRB afterglows per year, of which 1–3 have $z > 5$ . These rates represent a substantial contribution to the field of high-z GRB science, as only 23 $z > 5$ GRBs have been collectively discovered by the entire astronomical community over the last ${\sim}24$ yr. Future discoveries are critically needed to take advantage of next generation follow-up spectroscopic facilities such as 30m-class ground telescopes and the James Webb Space Telescope. Furthermore, a systematic space-based follow-up of afterglows in the near-infrared will offer new insight on the population of dusty (‘dark’) GRBs which are primarily found at cosmic noon ( $z\sim 1-3$ ). Additionally, we find that launching a mini-constellation of 3 near-infrared nano-satellites would increase the detection fraction of afterglows to ${\sim}83\%$ and substantially reduce the latency in the photometric redshift determination.

Whether monozygotic (MZ) and dizygotic (DZ) twins differ from each other in a variety of phenotypes is important for genetic twin modeling and for inferences made from twin studies in general. We analyzed whether there were differences in individual, maternal and paternal education between MZ and DZ twins in a large pooled dataset. Information was gathered on individual education for 218,362 adult twins from 27 twin cohorts (53% females; 39% MZ twins), and on maternal and paternal education for 147,315 and 143,056 twins respectively, from 28 twin cohorts (52% females; 38% MZ twins). Together, we had information on individual or parental education from 42 twin cohorts representing 19 countries. The original education classifications were transformed to education years and analyzed using linear regression models. Overall, MZ males had 0.26 (95% CI [0.21, 0.31]) years and MZ females 0.17 (95% CI [0.12, 0.21]) years longer education than DZ twins. The zygosity difference became smaller in more recent birth cohorts for both males and females. Parental education was somewhat longer for fathers of DZ twins in cohorts born in 1990–1999 (0.16 years, 95% CI [0.08, 0.25]) and 2000 or later (0.11 years, 95% CI [0.00, 0.22]), compared with fathers of MZ twins. The results show that the years of both individual and parental education are largely similar in MZ and DZ twins. We suggest that the socio-economic differences between MZ and DZ twins are so small that inferences based upon genetic modeling of twin data are not affected.

A trend toward greater body size in dizygotic (DZ) than in monozygotic (MZ) twins has been suggested by some but not all studies, and this difference may also vary by age. We analyzed zygosity differences in mean values and variances of height and body mass index (BMI) among male and female twins from infancy to old age. Data were derived from an international database of 54 twin cohorts participating in the COllaborative project of Development of Anthropometrical measures in Twins (CODATwins), and included 842,951 height and BMI measurements from twins aged 1 to 102 years. The results showed that DZ twins were consistently taller than MZ twins, with differences of up to 2.0 cm in childhood and adolescence and up to 0.9 cm in adulthood. Similarly, a greater mean BMI of up to 0.3 kg/m2 in childhood and adolescence and up to 0.2 kg/m2 in adulthood was observed in DZ twins, although the pattern was less consistent. DZ twins presented up to 1.7% greater height and 1.9% greater BMI than MZ twins; these percentage differences were largest in middle and late childhood and decreased with age in both sexes. The variance of height was similar in MZ and DZ twins at most ages. In contrast, the variance of BMI was significantly higher in DZ than in MZ twins, particularly in childhood. In conclusion, DZ twins were generally taller and had greater BMI than MZ twins, but the differences decreased with age in both sexes.

For over 100 years, the genetics of human anthropometric traits has attracted scientific interest. In particular, height and body mass index (BMI, calculated as kg/m2) have been under intensive genetic research. However, it is still largely unknown whether and how heritability estimates vary between human populations. Opportunities to address this question have increased recently because of the establishment of many new twin cohorts and the increasing accumulation of data in established twin cohorts. We started a new research project to analyze systematically (1) the variation of heritability estimates of height, BMI and their trajectories over the life course between birth cohorts, ethnicities and countries, and (2) to study the effects of birth-related factors, education and smoking on these anthropometric traits and whether these effects vary between twin cohorts. We identified 67 twin projects, including both monozygotic (MZ) and dizygotic (DZ) twins, using various sources. We asked for individual level data on height and weight including repeated measurements, birth related traits, background variables, education and smoking. By the end of 2014, 48 projects participated. Together, we have 893,458 height and weight measures (52% females) from 434,723 twin individuals, including 201,192 complete twin pairs (40% monozygotic, 40% same-sex dizygotic and 20% opposite-sex dizygotic) representing 22 countries. This project demonstrates that large-scale international twin studies are feasible and can promote the use of existing data for novel research purposes.

Mice in mid lactation (n=6 per group) were fed a control diet (1·19% fat), or diets containing safflower oil (25% w/w) or olive oil (25% w/w) for 7 d. Mammary and liver stearoyl CoA desaturase (SCD) mRNA levels and mammary SCD activities were higher in lactating mice fed the control diet than in those fed the oil-supplemented diets. Further, mammary SCD mRNA was directly related to mammary SCD activity. Milk fat composition was influenced by dietary fat composition. The olive oil diet, high in 18[ratio ]1, led to high levels of this fatty acid in milk and the safflower oil diet, high in 18[ratio ]2, resulted in a milk fat with high levels of 18[ratio ]2. These results show that there is regulation of SCD at the transcriptional level, associated with changes in enzyme activity and in milk fat composition.

We describe the design of Escherichia coli cells that synthesize a structurally perfect, recombinant cytochrome c from the Thermus thermophilus cytochrome c552 gene. Key features are (1) construction of a plasmid-borne, chimeric cycA gene encoding an Escherichia coli-compatible, N-terminal signal sequence (MetLysIleSerIleTyrAlaThrLeuAlaAlaLeuSerLeuAlaLeuProAlaGlyAla) followed by the amino acid sequence of mature Thermus cytochrome c552; and (2) coexpression of the chimeric cycA gene with plasmid-borne, host-specific cytochrome c maturation genes (ccmABCDEFGH). Approximately 1 mg of purified protein is obtained from 1 L of culture medium. The recombinant protein, cytochrome rsC552, and native cytochrome c552 have identical redox potentials and are equally active as electron transfer substrates toward cytochrome ba3, a Thermus heme-copper oxidase. Native and recombinant cytochromes c were compared and found to be identical using circular dichroism, optical absorption, resonance Raman, and 500 MHz 1H-NMR spectroscopies. The 1.7 Å resolution X-ray crystallographic structure of the recombinant protein was determined and is indistinguishable from that reported for the native protein (Than, ME, Hof P, Huber R, Bourenkov GP, Bartunik HD, Buse G, Soulimane T, 1997, J Mol Biol 271:629–644). This approach may be generally useful for expression of alien cytochrome c genes in E. coli.