The analysis of microsatellite loci has allowed significant advances in evolutionary biology and pest management. However, until very recently, the potential benefits have been compromised by the high costs of developing these neutral markers. High-throughput sequencing provides a solution to this problem. We describe the development of 13 microsatellite markers for the eusocial ambrosia beetle, Austroplatypus incompertus, a significant pest of forests in southeast Australia. The frequency of microsatellite repeats in the genome of A. incompertus was determined to be low, and previous attempts at microsatellite isolation using a traditional genomic library were problematic. Here, we utilised two protocols, microsatellite-enriched genomic library construction and high-throughput 454 sequencing and characterised 13 loci which were polymorphic among 32 individuals. Numbers of alleles per locus ranged from 2 to 17, and observed and expected heterozygosities from 0.344 to 0.767 and from 0.507 to 0.860, respectively. These microsatellites have the resolution required to analyse fine-scale colony and population genetic structure. Our work demonstrates the utility of next-generation 454 sequencing as a method for rapid and cost-effective acquisition of microsatellites where other techniques have failed, or for taxa where marker development has historically been both complicated and expensive.

Retinae of the crab Leptograpsus which had been maintained on a 12-h light/12-h dark cycle were cultured in vitro and exposed to 1 μM okadaic acid (OKA) at 0.75 h before light onset. Control retinae were subjected to the same routine and sampled at the same times without OKA treatment. At the concentration used, OKA totally inhibits types 1 and 2A protein phosphatases, minimally inhibits type 2B, and does not affect type 2C. 1 μM OKA provoked a diminution of rhabdom diameter measured at the level of the photoreceptor nuclei in the dark, some ommatidial cartridges being stripped of rhabdomeral microvilli altogether. After 1-h illumination (225–320 lux), further reduction of rhabdom diameter was modest in control retinae but precipitate in those treated with OKA. After 2 h, control rhabdom diameters showed a further, not significant, decline, but OKA had induced a resynthesis of massive structures with the light-microscopic appearance of rhabdoms. Electron microscopy revealed that they were heterogeneous and of the following kinds: (1) a minority of rhabdoms with normally disposed but distorted microvilli; (2) rhabdoms in the throes of events that parody normal assembly; and (3) rhabdomal volumes occupied by saccular organelles or by pleats or ruffles of irregular architecture. The cytoplasm of all such receptors was packed with free and bound ribosomes and endomembranes. The sequence of events parallels that seen during light-induced degeneration of photoreceptors of the Drosophila mutant w rdgBKS222. Preliminary experiments show that a protein kinase activator SC-9 mimics many of these effects in the dark in the presence of 1 μM OKA. As a working hypothesis, it is proposed that light activates protein kinases via diacylglycerols generated by the phototransduction cascade, and that in both crab retinas challenged with OKA and retinas of rdgBKS222 activation of a nuclear regulatory protein by hyperphosphorylation provokes a runaway transcription whose selectivity and extent remain to be determined.

Focusing of heavy-ion beams is an important issue for ion beam-driven inertial confinement fusion. For the experimental program to investigate matter at high energy densities at GSI, the application of a plasma lens has attractive features compared to standard quadrupole lenses. A plasma lens using a wall-stabilized discharge has been systematically investigated and optimized for this purpose. Different lenses were tested in several runs at the GSI linear accelerator UNILAC and at the SIS-synchrotron. A remarkably high accuracy and reproducibility of the focusing were found. The focal spot size was mainly limited by the beam emittance. A summary of experimental results and important limitations of the focal spot size is given.

The main objective of the experimental plasma physics activities at the Gesellschaft für Schwerionenforschung (GSI) is the interaction processes of heavy ions with dense ionized matter. Gas discharge plasma targets were used for energy loss and charge state measurements in a regime of electron density and temperature up to 1019 cm-3 and 20 eV, respectively. Progress has been achieved in the understanding of charge-exchange processes in fully ionized hydrogen plasma. An improved model taking excitation-autoionization processes into account has removed most of the discrepancies of previous theoretical descriptions. Furthermore, it was found that the energy loss of the ion beam serves as an excellent diagnostic tool for measuring the electron density in partially ionized plasmas such as argon. The experience with these methods will be used in the future to diagnose dense laser produced plasmas. A setup with a 100 J/5 GW Nd:glass laser, currently under construction, will provide access to density range up to 1021 cm-3 and temperatures of more than 100 eV. To reach electron densities near solid-state density (1023 cm-3), heavy ion heated frozen rare gas crystals were used. The first hydrodynamic motion of ion heated solid material was observed. Vacuum-ultraviolet (VUV) spectroscopy was applied to diagnose these strongly coupled nonideal plasmas.

Extended abstract of a paper presented at Microscopy and Microanalysis 2007 in Ft. Lauderdale, Florida, USA, August 5 – August 9, 2007

This paper applies recent advances in ecology to our understanding of firm development, sustainability, and economic development. The ecological literature indicates that the greater the functional richness of species in a system, the greater its resilience – that is, its ability to persist in the face of substantial changes in the environment. This paper focuses on the effects of functional richness across firm size on the ability of industries to survive in the face of economic change. Our results indicate that industries with a richness of industrial functions are more resilient to employment volatility.

We consider a class of three-dimensional boundary-layer flows, which may be viewed as an extension of the Falkner–Skan similarity form, to include a cross-flow velocity component, about a plane of symmetry. In general, this provides a range of three- dimensional boundary-layer solutions, parameterized by a Falkner–Skan similarity parameter, n, together with a further parameter, Ψ∞, which is associated with a cross-flow velocity component in the external flow. In this work two particular cases are of special interest: for n = 0 the similarity equations possess a family of solutions related to the Blasius boundary layer; for n = 1 the similarity solution provides an exact reduction of the Navier–Stokes equations corresponding to the flow near a saddle point of attachment. It is known from the work of Davey (1961) that in this latter class of flow, a continuum of solutions can be found. The continuum arises (in general) because it is possible to find states with an algebraic, rather than exponential, behaviour in the far field. In this work we provide a detailed overview of the continuum states, and show that a discrete infinity of ‘exponential modes’ are smoothly embedded within the ‘algebraic modes’ of the continuum. At a critical value of the cross-flow, these exponential modes appear as a cascade of eigensolutions to the far-field equations, which arise in a manner analogous to the energy eigenstates found in quantum mechanical problems described by the Schrödinger equation.

The presence of a discrete infinity of exponential modes is shown to be a generic property of the similarity equations derived for a general n. Furthermore, we show that there may also exist non-uniqueness of the continuum; that is, more than one continuum of states can exist, that are isolated for fixed n and Ψ∞, but which are connected through an unfolded transcritical bifurcation at a critical value of the cross-flow parameter, Ψ∞.

The multiplicity of states raises the question of solution selection, which is addressed using two stability analyses that assume the same basic symmetry properties as the base flow. In one case we consider a steady, algebraic form in the ‘streamwise’ direction, whilst in the other a temporal form is assumed. In both cases it is possible to extend the analysis to consider a continuous spectrum of disturbances that decay algebraically in the wall-normal direction. We note some obvious parallels that exist between such stability analyses and the approach to the continua of states described earlier in the paper.

We also discuss the appearance of analogous non-unique states to the Falkner–Skan equation in the presence of an adverse pressure gradient (i.e. n < 0) in an appendix.

Small perturbations of a choked flow through a thin annular nozzle are investigated. Two cases are considered, corresponding to a ‘choked outlet’ and a ‘choked inlet’ respectively. For the first case, either an acoustic or entropy or vorticity wave is assumed to be travelling downstream towards the nozzle contraction. An asymptotic analysis for low frequency is used to find the reflected acoustic wave that is created. The boundary condition found by Marble & Candel (1977) for a compact choked nozzle is shown to apply to first order, even for circumferentially varying waves. The next-order correction can be expressed as an ‘effective length’ dependent on the mean flow (and hence the particular geometry of the nozzle) in a quantifiable way.

For the second case, an acoustic wave propagates upstream and is reflected from a convergent–divergent nozzle. A normal shock is assumed to be present. By considering the interaction of the shock's position and flow perturbations, the reflected propagating waves are found for a compact nozzle. It is shown that a significant entropy disturbance is produced even when the shock is weak, and that for circumferential modes a vorticity wave is also present. Numerical calculations are conducted using a sample geometry and good agreement with the analysis is found at low frequency in both cases, and the range of validity of the asymptotic theory is determined.

The hydrodynamic response of metal targets to volume heating by energy deposition of intense heavy-ion beams was investigated experimentally. Recent improvements in beam parameters led to a marked increase in specific deposition power: 2·101040Ar18+ ions of 300 MeV/u focused to a spot size of 300 μm (σ) × 540 μm (σ) yield a specific deposition energy in solid lead of approximately 1 kJ/g in the Bragg peak, delivered within 250 ns [full width at half maximum (FWHM)]. This value allowed us for the first time to observe heavy-ion-beam-induced hydrodynamic expansion of metal volume targets. Measurements comprise expansion velocities of free surfaces of up to 290 ± 20 m/s, surface temperatures of ejected target matter of 1600–1750 K, and pressure waves in solid metal bulk targets of 0.16 GPa maximum absolute value and 0.8 μs FWHM. The experimental results agree well with the results of a 2D hydrodynamic code. Inside the interaction zone, which can only be accessed by simulation, maximum temperatures are 2800 K and maximum pressures are 3.8 GPa.

Background and objective Volatile anaesthetic effects on altering tone after blocking nitric oxide synthase, cyclo-oxygenase-prostaglandin synthase and KATP channel pathways are controversial. We examined in isolated guinea pig hearts whether anaesthetics alter bradykinin and 5-hydroxytryptamine-induced effects on coronary flow and percentage oxygen extraction after blocking these pathways.

Methods Before and during exposure to sevo-flurane, halothane or isoflurane, hearts were infused with 10−13–10−8 M bradykinin, or 10−8–10−6 M 5-hydroxytryptamine (serotonin), with either L-NAME, indomethacin, or glibenclamide. Bradykinin or 5-hydroxytryptamine alone increased flow and decreased percentage oxygen extraction in a concentration-dependent manner; these effects were largely blocked by L-NAME (nitro-L-arginine methylester), which also decreased basal flow and increased basal percentage oxygen extraction.

Results The anaesthetics restored bradykinin and 5-hydroxytryptamine-induced increases in flow or decreases in percentage oxygen extraction after inhibition by L-NAME. Indomethacin or glibenclamide alone had little effect on basal flow and percentage oxygen extraction. The anaesthetics restored bradykinin and 5-hydroxytryptamine-induced increases in flow or decreases in percentage oxygen extraction after inhibition by L-NAME. Indomethacin or glibenclamide alone had little effect on basal flow and percentage oxygen extraction. Drug-induced increases in flow and decreases in percentage oxygen extraction in the absence or presence of glibenclamide or indomethacin were not altered at either of the two concentrations of anaesthetics.

Conclusions Endothelium-dependent vasodilatation is not affected by blocking prostaglandin release or KATP channels in the intact heart even in the presence of an anaesthetic. However, the diminished responses to vasodilators after nitric oxide synthase inhibition is largely restored or enhanced by anaesthetics.