The Ladinian–Carnian transition in the Tethys domain was accompanied by an important environmental change representing a milestone in the climate evolution of the Triassic. However, estimations on paleodiversity composition and paleoenvironmental conditions across this interval are scarce in marine settings due to the lack of fossil-bearing successions. In this work, a refined paleontological and sedimentological study has allowed us to better characterize a well-preserved marine ?Ladinian–Carnian carbonate succession in the South Central Pyrenees (Odèn site, Catalonia, NE Spain). Vertebrate faunas include numerous actinopterygian specimens, forming an assemblage composed of at least four taxa: Peltopleurus cf. P. nuptialis Lombardo, 1999, Saurichthys sp., Colobodus giganteus (Beltan, 1972), and an indeterminate halecomorph. Specimens belonging to the genus Peltopleurus are dominant; the long-snouted Saurichthys, the halecomorph, and the large-bodied Colobodus giganteus are less abundant. Tetrapod remains are scarcely present and are assigned to sauropterygians. Invertebrate faunas include bivalves (Pseudocorbula gregaria [Münster in Goldfuss, 1838]) and brachiopods (Lingula sp.). The fossil assemblage was recovered from organic-rich laminated silty mudstone layers. Sedimentological and textural analyses suggest that fossil biotas were deposited below the fair-weather wave base in shallow subtidal coastal settings. These environments were sporadically sourced by silt/clay. The age of the Odèn site, on the basis of the recovered fauna, is assigned to the ?late Ladinian–middle Carnian (Middle–Late Triassic), which is in agreement with previously published ages based on palynomorph data.

The refined integration of paleontological, sedimentological, and biostratigraphic data from the Odèn site and other vertebrate-bearing localities in the Tethys domain can help better constrain the paleoenvironmental conditions and paleogeographical configuration impacting ecosystem diversity during the late Ladinian–Carnian interval.

The burden of depression is increasing worldwide, specifically in older adults. Unhealthy dietary patterns may partly explain this phenomenon. In the Spanish PREDIMED-Plus study, we explored (1) the cross-sectional association between the adherence to the Prime Diet Quality Score (PDQS), an a priori-defined high-quality food pattern, and the prevalence of depressive symptoms at baseline (cross-sectional analysis) and (2) the prospective association of baseline PDQS with changes in depressive symptomatology after 2 years of follow-up. After exclusions, we assessed 6612 participants in the cross-sectional analysis and 5523 participants in the prospective analysis. An energy-adjusted high-quality dietary score (PDQS) was assessed using a validated FFQ. The cross-sectional association between PDQS and the prevalence of depression or presence of depressive symptoms and the prospective changes in depressive symptoms were evaluated through multivariable regression models (logistic and linear models and mixed linear-effects models). PDQS was inversely associated with depressive status in the cross-sectional analysis. Participants in the highest quintile of PDQS (Q5) showed a significantly reduced odds of depression prevalence as compared to participants in the lowest quartile of PDQS (Q1) (OR (95 %) CI = 0·82 (0·68, 0·98))). The baseline prevalence of depression decreased across PDQS quintiles (Pfor trend = 0·015). A statistically significant association between PDQS and changes in depressive symptoms after 2-years follow-up was found (β (95 %) CI = −0·67 z-score (–1·17, −0·18). A higher PDQS was cross-sectionally related to a lower depressive status. Nevertheless, the null finding in our prospective analysis raises the possibility of reverse causality. Further prospective investigation is required to ascertain the association between PDQS and changes in depressive symptoms along time.

While life expectancy increases in developed countries and there is evidence that demonstrates the potential of the internet to optimise or compensate for the losses associated with ageing, there is a high proportion of older people who continue to be disconnected from the digital world. In this scenario, the technological support offered by public institutions has the potential to be an accessible source for the digital literacy of older people. This study, using the model of digital inequality, had the aim of analysing the ability of these institutional supports to determine and predict the digital inclusion of older people. The sample was retired adults (over 54 years) residing in Spain who are users of technological support services in four organisational contexts: nursing homes, senior community centres, University Programs for Seniors and adult education programmes. Through binary logistic regression analysis, we found that the ability of the availability of literacy support to determine and predict access, autonomy, skills and use of the internet for social connectivity depends on the social and organisational context of the technology support service. These findings support empirically the situated nature of technological support for the digital inclusion of older people and provide a useful comparative vision for the design of accessible support services adapted to the needs of its users.

Rhizoma perennial peanut (RPP) is well adapted to the Gulf Coast region of the United States, but its varietal tolerance to glyphosate and triclopyr is not well defined. The research was conducted to determine the effect of various rates of glyphosate and triclopyr on established RPP, and the response of common RPP varieties to these herbicides. The RPP sward was approximately 7 yr younger at Zolfo Springs than at the Ona location. RPP showed moderate tolerance to glyphosate and triclopyr application, and injury level did not differ with the age of RPP sward. However, biomass production was negatively influenced by the age of the RPP sward. Overall, injury from glyphosate applications did not exceed 40% at either site. The glyphosate rate for 20% biomass reduction was predicted to be 0.53 and 2.17 kg ae ha−1 at Zolfo Springs and Ona, respectively. RPP injury from triclopyr was greater at the Zolfo Springs location than at Ona, and the triclopyr rate predicted to result in a 20% biomass reduction was 0.45 and 0.99 kg ae ha−1 at the Zolfo Springs and Ona locations, respectively. There was a difference on RPP varieties response to glyphosate and triclopyr application. ‘Florigraze’ and ‘Ona 33’ were less tolerant to glyphosate compared to ‘UF-Tito’ and ‘Ecoturf’ at 30 d after treatment. Likewise, UF-Tito and Florigraze were less tolerant to triclopyr compared to Ona 33 and Ecoturf. Overall, Florigraze showed highest injury and at least 2-fold reduction on biomass compared to the other three varieties from glyphosate or triclopyr application. Results from this research indicate that glyphosate and triclopyr appear to be safe to apply to long-established RPP stands, but herbicide rate and RPP varieties should be considered if stands are <5 yr old.

VmCT1 is an antimicrobial peptide (AMP) isolated from the venom of the scorpion Vaejovis mexicanus with antimicrobial, anticancer and antimalarial activities, which the rational design with Arg-substitution has yielded AMPs with higher antimicrobial activity than VmCT1. Chagas is a neglected tropical disease, becoming the development of new antichagasic agents is urgent. Thus, we aimed to evaluate the antichagasic effect of VmCT1 and three Arg-substituted analogues, as well their action mechanism. Peptides were tested against the epimastigote, trypomastigote, amastigote forms of Trypanossoma cruzi Y strain and against LLC-MK2 mammalian cells. The mechanism of action of these peptides was evaluated by means of flow cytometry and scanning electron microscopy. VmCT1 presented activity against all three forms of T. cruzi, with EC50 against trypomastigote forms of 1.37 μmol L−1 and selectivity index (SI) of 58. [Arg]3-VmCT1, [Arg]7-VmCT1 and [Arg]11-VmCT1 also showed trypanocidal effect, but [Arg]11-VmCT1 had the best effect, being able to decrease the EC50 against trypomastigote forms to 0.8 μmol L−1 and increase SI to 175. Necrosis was cell death pathway of VmCT1, as well [Arg]7-VmCT1 and [Arg]11-VmCT1, such as observed by membrane damage in flow cytometry analyses and scanning-electron-microscopy. In conclusion, [Arg]11-VmCT1 revealed promising as a candidate for new antichagasic therapeutics.

Pintoi peanut is a warm-season perennial legume that shows promise as a forage crop for the southeastern United States, however, little is known about the proper methods of weed management during establishment for this species. The objective of this study was to determine the ability of pintoi peanut to tolerate applications of PRE and POST herbicides during the year of and year after planting. The effects of herbicide treatments on percentage of visual estimates of injury and stand counts of pintoi peanut were investigated at Ona and Marianna, FL, in 2015 and 2016. All PRE herbicides did not result in significant injury or stand reduction. Pintoi peanut’s tolerance to POST herbicides was higher when plants were emerged for at least 2 wk prior to herbicide application. Stands of pintoi peanut that were planted the previous year appear to tolerate all herbicides examined in this work, except sulfosulfuron. Results of this study indicate that at the year of planting pintoi peanut is tolerant to PRE applications of pendimethalin, imazethapyr, and imazapic. Pintoi peanut appears to tolerate applications of 2,4-D, carfentrazone, imazapic and imazethapyr the year after planting at the rates utilized in this study. Future research should evaluate the effects of multiple herbicide applications and tank-mixes to obtain satisfactory weed control and selectivity in pintoi peanut swards.

The random encounter model, a method for estimating animal density using camera traps without the need for individual recognition, has been developed over the past decade. A key assumption of this model is that cameras are placed randomly in relation to animal movements, requiring that cameras are not set only at sites thought to have high animal traffic. The aim of this study was to define a correction factor that allows the random encounter model to be applied in photo-trapping surveys in which cameras are placed along tracks to maximize capture probability. Our hypothesis was that applying such a correction factor would compensate for the different rates at which lynxes use tracks and the surrounding area, and should thus improve the estimates obtained with the random encounter model. We tested this using data from a well-known Iberian lynx Lynx pardinus population. Firstly, we estimated Iberian lynx densities using a traditional camera-trapping design followed by spatially explicit capture–recapture analyses. We estimated the differential use rate for tracks vs the surrounding area using data from a lynx equipped with a GPS collar, and subsequently calculated the correction factor. As expected, the random encounter model overestimated densities by 378%. However, the application of the correction factor improved the estimate and reduced the error to 16%. Although there are limitations to the application of the correction factor, the corrected random encounter model shows potential for density estimation of species for which individual identification is not possible.

The aim of this piece of research was to study the existence of clusters based on anger, empathy and cortisol and testosterone measures associated with aggressive behavior in school-aged children. The sample group comprised 139 eight-year-old children (80 boys and 59 girls). Aggressive behavior was measured using the Direct and Indirect Aggression Scale. Both psychological and biological variables were used to determine psychobiological profiles. The psychological variables considered were trait anger, measured using the State-Trait Anger Expression Inventory for Children and Adolescents, and empathy, measured using the Empathy Quotient-Child Version. Testosterone and cortisol concentrations were measured through saliva samples and analyzed using an ELISA (Enzyme-linked immunosorbent assay). A Cluster Analysis revealed three clusters which were clearly different as regards their psychological and biological characteristics. The analysis of variance (ANOVA) revealed that the cluster characterized by having higher anger levels, lower empathy levels and higher testosterone and cortisol levels was more aggressive than the other two (p < .0001, η2 = .19). The results indicate that studying psychological and biological variables together may help establish differentiated aggression patterns among children.

Chagas disease is a public health problem, affecting about 7 million people worldwide. Benznidazole (BZN) is the main treatment option, but it has limited effectiveness and can cause severe adverse effects. Drug delivery through nanoparticles has attracted the interest of the scientific community aiming to improve therapeutic options. The aim of this study was to evaluate the cytotoxicity of benznidazole-loaded calcium carbonate nanoparticles (BZN@CaCO3) on Trypanosoma cruzi strain Y. It was observed that BZN@CaCO3 was able to reduce the viability of epimastigote, trypomastigote and amastigote forms of T. cruzi with greater potency when compared with BZN. The amount of BZN necessary to obtain the same effect was up to 25 times smaller when loaded with CaCO3 nanoparticles. Also, it was observed that BZN@CaCO3 enhanced the selectivity index. Furthermore, the cell-death mechanism induced by both BZN and BZN@CaCO3 was evaluated, indicating that both substances caused necrosis and changed mitochondrial membrane potential.

Cathelicidins are antimicrobial peptides produced by humans and animals in response to various pathogenic microbes. Crotalicidin (Ctn), a cathelicidin-related vipericidin from the South American Crotalus durissus terrificus rattlesnake's venom gland, and its fragments have demonstrated antimicrobial and antifungal activity, similarly to human cathelicidin LL-37. In order to provide templates for the development of modern trypanocidal agents, the present study evaluated the antichagasic effect of these four peptides (Ctn, Ctn[1-14], Ctn[15-34] and LL-37). Herein, Ctn and short derived peptides were tested against the epimastigote, trypomastigote and amastigote forms of Trypanosoma cruzi Y strain (benznidazole-resistant strain) and cytotoxicity in mammalian cells was evaluated against LLC-MK2 lineage cells. Ctn inhibited all T. cruzi developmental forms, including amastigotes, which is implicated in the burden of infection in the chronic phase of Chagas disease. Moreover, Ctn showed a high selective index against trypomastigote forms (>200). Ctn induced cell death in T. cruzi through necrosis, as determined by flow cytometry analyses with specific molecular probes and morphological alterations, such as loss of membrane integrity and cell shrinkage, as observed through scanning electron microscopy. Overall, Ctn seems to be a promising template for the development of antichagasic agents.

Leishmaniasis is one of the world's most neglected diseases, and it has a worldwide prevalence of 12 million. There are no effective human vaccines for its prevention, and treatment is hampered by outdated drugs. Therefore, research aiming at the development of new therapeutic tools to fight leishmaniasis remains a crucial goal today. With this purpose in mind, we present 20 arylaminoketone derivatives with a very interesting in vitro and in vivo efficacy against Trypanosoma cruzi that have now been studied against promastigote and amastigote forms of Leishmania infantum, Leishmania donovani and Leishmania braziliensis strains. Six out of the 20 Mannich base-type derivatives showed Selectivity Index between 39 and 2337 times higher in the amastigote form than the reference drug glucantime. These six derivatives affected the parasite infectivity rates; the result was lower parasite infectivity rates than glucantime tested at an IC25 dose. In addition, these derivatives were substantially more active against the three Leishmania species tested than glucantime. The mechanism of action of these compounds has been studied, showing a greater alteration in glucose catabolism and leading to greater levels of iron superoxide dismutase inhibition. These molecules could be potential candidates for leishmaniasis chemotherapy.

The phenological development of crops from emergence to flowering time is largely controlled by temperature and photoperiod. Flowering time is a critical phenological stage for subsequent reproductive phase. Lotus tenuis management in grasslands, pastures and seed production systems is through defoliation and sowing date; however, yet little is known about their effects on flowering time. The data presented in this study were obtained from experiments conducted with L. tenuis during the years 1989 to 2016 under field conditions. Our objectives were to determine if flowering time (a) is affected by sowing date; (b) can be predicted through equations using temperature and photoperiod and (c) is affected by defoliation applied at vegetative stage. Two defoliation intensities were applied, low (LDI) crop height reduced by 54% compared to pre-defoliation crop height and high (HDI), crop height reduced by 75%. The rate of progress from seedling emergence to flowering time (inverse of time from emergence to first flowering, 1/f) was modulated by temperature, photoperiod and photothermal functions. When L. tenuis sowing was delayed from autumn to spring, time from seedling emergence to first flowering decreased from 260 to 100 days. 1/f was linearly related to average temperature (R²=0.75) and photoperiod (R²=0.85) and both variables (R²=0.92). Defoliation retarded flowering time. Flower and pod growth periods were shorter under defoliation than in control one. Defoliation did not cause abortion of flowers and pods. Flower production was fitted to quadratic function of photoperiod. Flowering peak was approximately within 15.2 h. The prediction of flowering time using thermal, photoperiod and photothermal models can provide information about crop management decisions, such as optimal environmental regimes for crop growth through sowing date.

During 2004 to 2008, weed surveys were conducted in 373 wheat fields of two different cropped areas (southwest [SW] and southeast [SE]) of the southern region of Buenos Aires Province of Argentina where different weed communities were expected because of changes in cropping practices over time, including tillage, crop sequence, fertilizers, and herbicides applied. Weed communities differed between regions, with greater numbers of native species for the SW. Weed community diversity was also greater for the SW region, probably due to the more diverse land use that resulted in greater landscape heterogeneity. Rush skeletonweed, sand rocket, yellow starthistle and turnipseed occurred at higher constancy (proportion of fields in which a given species is present) in the SW region, whereas common chickweed, false bishop's weed, corn speedwell, and common lambsquarters were present more frequently in the SE region. Compared with the 1982 survey, constancy of weeds increased, but those species with high constancy in 1982 were also with high constancy in the recent surveys. Diversity (species richness) was greater in conventional than in a no-tillage system. The constancy of Italian ryegrass, sand rocket, and yellow starthistle was lower under no-till than conventional tillage. Surveys allow identification of changes in weed community related to different agricultural systems. Rotation of crops and livestock avoid the homogenization of the environment at the landscape level. Management strategies will be necessary to prevent the increase of weeds populations' size, preserving plant diversity and the properties of the agroecosystem.

A mother’s emotional state is a well-known environmental factor that relates to the development of infant temperament. However, some relevant issues have not yet been fully explored. The current study examines the influence of determined maternal, contextual and perinatal variables on infant temperament and the mother’s confidence in caregiving during the first weeks of life. A prospective study was carried out in three-hundred and seventeen newborns and their mothers. Perinatal and socio-demographic variables were recorded. The mother’s anxiety and mood were measured in the first days after childbirth and again at 8 weeks. Infant temperament and the mother’s confidence in caregiving were measured at 8 weeks. A mother’s postpartum anxiety following delivery was the best predictor for most of the variables of infant temperament, including infant irritability (p = .001), and other child variables like infant sleep (p = .0003) and nursing difficulty (p = .001). Contextual-family variables, such as the number of people at home (p = .0024) and whether they were primiparous (p = .001), were the best predictors for a mother´s confidence in caregiving. Support was found for an early effect of maternal anxiety on infant temperament. The results have clinical implications for postnatal psychological interventions.

Moderate alcohol intake has been related to lower mortality. However, alcohol use includes other dimensions beyond the amount of alcohol consumed. These aspects have not been sufficiently studied as a comprehensive entity. We aimed to test the relationship between an overall alcohol-drinking pattern and all-cause mortality. In a Mediterranean cohort study, we followed 18 394 Spanish participants up to 12 years. A validated 136-item FFQ was used to assess baseline alcohol intake. We developed a score assessing simultaneously seven aspects of alcohol consumption to capture the conformity to a traditional Mediterranean alcohol-drinking pattern (MADP). It positively scored moderate alcohol intake, alcohol intake spread out over the week, low spirit consumption, wine preference, red wine consumption, wine consumed during meals and avoidance of binge drinking. During the follow-up, 206 deaths were identified. For each 2-point increment in a 0–9 score of adherence to the MADP, we observed a 25 % relative risk reduction in mortality (95 % CI 11, 38 %). Within each category of alcohol intake, a higher adherence to the MADP was associated with lower mortality. Abstainers (excluded from the calculations of the MADP) exhibited higher mortality (hazard ratio 1·82, 95 % CI 1·14, 2·90) than participants highly adherent to the MADP. In conclusion, better adherence to an overall healthy alcohol-drinking pattern was associated with reduced mortality when compared with abstention or departure from this pattern. This reduction goes beyond the inverse association usually observed for moderate alcohol drinking. Even moderate drinkers can benefit from the advice to follow a traditional MADP.