Land-use change for crop production is one of the key drivers of habitat loss and fragmentation and consequently biodiversity loss and change in tropical regions. This may impact biodiversity-regulated ecosystem services; birds are important to crop health regulating services (e.g. seed dispersal, pest control) and disservices (e.g. seed predation, grain herbivory). However, knowledge is limited on how birds use heterogeneous agricultural landscapes and the consequences for spatial distribution and flow of services and disservices. We studied crop and non-crop–habitat associations of birds in forest–agricultural landscapes of the Kilombero Valley, Tanzania. We focused on dietary preference as a key trait impacting bird responses to land-use change, services, and disservices to crops. We surveyed birds across four main habitat types using repeated point counts, recording a total of 148 species. We found that crop habitats supported higher species richness and larger communities of potentially beneficial species to crop health, whereby 34.5% of invertebrate-feeding species were recorded in cropland. We found that habitat heterogeneity within the landscape supports bird functional diversity and that each habitat type supported unique communities of species. Furthermore, the number of species unique to forest habitats increased with increasing forest canopy closure. Our findings suggest that management strategies for maintaining trees and shrubs, and enhancing tree cover within the crop production landscape, can be effective approaches for maintaining bird diversity and services. However, in-depth studies on trade-offs with disservices need further exploration to mitigate negative impacts of birds on crop yields.

Experiments were conducted to study the transition and flow development in a laminar separation bubble (LSB) formed on an aerofoil. The effects of a wide range of free-stream turbulence intensity ($0.15\,\%< Tu<6.26\,\%$) and streamwise integral length scale ($4.6\ {\rm mm}<\varLambda _{u}<17.2\ {\rm mm}$) are considered. The co-existence of modal instability due to the LSB and non-modal instability caused by streaks generated by free-stream turbulence is observed. The flow field is measured using hot-wire anemometry, which showed that the presence of streaks in the boundary layer modifies the mean-flow topology of the bubble. These changes in the mean flow field result in the modification of the convective disturbance growth, where an increase in turbulence intensity is found to dampen the growth of the modal instability. For a relatively fixed level of $Tu$, the variation of $\varLambda _{u}$ has modest effects. However, a slight advancement of the nonlinear growth of disturbances and eventual breakdown with the decrease in $\varLambda _{u}$ is observed. The data show that the streamwise growth of the disturbance energy is exponential for the lowest levels of free-stream turbulence and gradually becomes algebraic as the level of free-stream turbulence increases. Once a critical turbulence intensity is reached, there is enough energy in the boundary layer to suppress the laminar separation bubble, resulting in the non-modal instability taking over the transition process. Linear stability analysis is conducted in the fore position of the LSB. It accurately models incipient disturbance growth, unstable frequencies and eigenfunctions for configurations subjected to turbulence intensity levels up to 3 %, showing that the mean-flow modification due to the non-modal instability dampens the modal instability.

ABSTRACT IMPACT: Plasma and CSF are not reliable estimates of drug exposure in tissue compartments relevant for treatment and prevention of infectious diseases. OBJECTIVES/GOALS: Globally, high dose fluconazole is widely used in the management of cryptococcal meningitis. While it is known to readily penetrate into cerebrospinal (CSF), less is known about drug concentrations in brain parenchymal tissues. Similarly, distribution of fluconazole into gynecological tissues has not been robustly characterized. METHODS/STUDY POPULATION: With informed consent from next-of-kin, we conducted autopsies within 24h of death for hospitalized Ugandans receiving fluconazole for treatment or secondary prophylaxis of cryptococcal meningitis. Dosing history was abstracted from medical chart and caregiver interviews. Fluconazole concentrations were determined using high-performance liquid chromatography- tandem mass spectrometry (LC-MS/MS) in plasma, CSF, 10 brain compartments (frontal, parietal, and occipital lobes, corpus callosum, globus pallidus, hippocampus, midbrain, medulla oblongata, spinal cord, and choroid plexus) and 4 female genital compartments (cervix, vagina, ovary, and uterus), depending on tissue availability. Descriptive statistics of tissue to plasma ratios were used to describe concentrations relative to plasma. RESULTS/ANTICIPATED RESULTS: Fluconazole concentrations were measured in available tissues of 21 individuals with detectable fluconazole in plasma. Daily doses of fluconazole were 200 mg (n=4), 400 mg (n=1), 800 mg (n=4), 1200 mg (n=9) or unknown (n=3). CSF concentrations (n=10) ranged from 93-1380% (median 100%) of plasma while brain concentrations (n=3) across all 10 compartments ranged from 45% to 89% (median 69%) of plasma. In the female genital tract, cervical concentrations (n=10) were 9-78% (median 65%) of plasma and in the 2 individuals with available tissue, concentrations in vaginal, ovarian, and uterine tissues were similar to cervix, ranging from 63-105% of plasma. DISCUSSION/SIGNIFICANCE OF FINDINGS: Measuring drug concentrations directly in tissues, the presumed site of action, improves estimates of drug efficacy. While fluconazole concentrations in CSF were similar to plasma, actual brain tissues were consistently lower. Concentrations were similar between upper and lower female genital tracts, but were consistently lower than plasma.

Mme L., 90 ans, était traitée en 3e intention par rispéridone 0,5 mg puis 1 mg durant 7 jours dans le cadre d’un trouble anxieux généralisé décompensé avec agitation. Une rhabdomyolyse et une cytolyse hépatique ont été découvertes fortuitement, associées à des signes cliniques de syndrome malin des neuroleptiques (SMNL) débutant. Les anomalies cliniques et paracliniques ont été résolutives 7 jours après l’arrêt de la rispéridone.

Nous avons réalisé une revue de la littérature sur le SMNL chez le sujet âgé de plus de 65 ans dans la base de données gratuite de Pubmed entre 1985 et 2014.

Parmi les patients retrouvés, la majorité étaient des hommes âgés de plus de 80 ans. Les pathologies traitées étaient représentées pour moitié par des pathologies neurodégénératives. Les différentes classes d’antipsychotiques étaient représentées, avec notamment : halopéridol, dropéridol, chlorpromazine, trifluopérazine, méthotrimeprazine, loxapine, sulpiride ainsi que risperidone et olanzapine. L’augmentation récente des doses semblait être un facteur favorisant. Les délais d’apparition étaient immédiat (< 48 h), précoce (2 à 15 jours), ou tardif (> 15 jours) dans la plupart des cas. Les signes cardinaux du SMNL étaient présents dans la plupart des cas, associés à une augmentation systématique des CPK. On note 50 % de mortalité.

La spécificité chez le sujet âgé semble être une mortalité plus élevée qu’en population générale. Les facteurs de risque tels les pathologies neurodégénératives sont analysés. Les auteurs interrogent le rapport bénéfice/risque de la prescription des antipsychotiques chez la personne âgée.

The European Exoplanets-A project aims to provide a comprehensive view of the nature of exoplanet atmospheres, through an interdisciplinary approach.

Exoplanets-A includes a knowledge server where we provide the scientific results and educational resources gathered and developed during the project. In this proceedings, we present two such educational resources: a MOOC and an augmented reality application.

The YBJ equation (Young & Ben Jelloul, J. Marine Res., vol. 55, 1997, pp. 735–766) provides a phase-averaged description of the propagation of near-inertial waves (NIWs) through a geostrophic flow. YBJ is obtained via an asymptotic expansion based on the limit $\mathit{Bu}\rightarrow 0$, where $\mathit{Bu}$ is the Burger number of the NIWs. Here we develop an improved version, the YBJ+ equation. In common with an earlier improvement proposed by Thomas, Smith & Bühler (J. Fluid Mech., vol. 817, 2017, pp. 406–438), YBJ+ has a dispersion relation that is second-order accurate in $\mathit{Bu}$. (YBJ is first-order accurate.) Thus both improvements have the same formal justification. But the dispersion relation of YBJ+ is a Padé approximant to the exact dispersion relation and with $\mathit{Bu}$ of order unity this is significantly more accurate than the power-series approximation of Thomas et al. (2017). Moreover, in the limit of high horizontal wavenumber $k\rightarrow \infty$, the wave frequency of YBJ+ asymptotes to twice the inertial frequency $2f$. This enables solution of YBJ+ with explicit time-stepping schemes using a time step determined by stable integration of oscillations with frequency $2f$. Other phase-averaged equations have dispersion relations with frequency increasing as $k^{2}$ (YBJ) or $k^{4}$ (Thomas et al. 2017): in these cases stable integration with an explicit scheme becomes impractical with increasing horizontal resolution. The YBJ+ equation is tested by comparing its numerical solutions with those of the Boussinesq and YBJ equations. In virtually all cases, YBJ+ is more accurate than YBJ. The error, however, does not go rapidly to zero as the Burger number characterizing the initial condition is reduced: advection and refraction by geostrophic eddies reduces in the initial length scale of NIWs so that $\mathit{Bu}$ increases with time. This increase, if unchecked, would destroy the approximation. We show, however, that dispersion limits the damage by confining most of the wave energy to low $\mathit{Bu}$. In other words, advection and refraction by geostrophic flows does not result in a strong transfer of initially near-inertial energy out of the near-inertial frequency band.

The pore structure of vapour deposited ASW is poorly understood, despite its importance to fundamental processes such as grain chemistry, cooling of star forming regions, and planet formation. We studied structural changes of vapour deposited D2O on intra-molecular to 30 nm length scales at temperatures ranging from 18 to 180 K and observed enhanced mobility from 100 to 150 K. An Arrhenius type model describes the loss of surface area and porosity with a common set of kinetic parameters. The low activation energy (428 K) is commensurate with van der Waals forces between nm-scale substructures in the ice. Our findings imply that water porosity will always change with time, even at low temperatures.