Residents in Stillwater County, MT, used a weed bounty program as an educational tool to promote awareness, detection, and control of spotted knapweed (Centaurea maculosa Lam. # CENMA) on range and noncropland. Many people became involved with the weed control effort, weed awareness was increased, spotted knapweed was controlled, and the program was cost effective. The success of a weed bounty program is influenced by 1) the enthusiasm and innovations of the county extension agent, weed supervisor, or other key individual or group in the community; 2) the choice of the weeds selected; and 3) good media coverage of the bounty program. With proper planning and organization, weed bounty programs effectively promote awareness and action in other areas of public concern.

Dicamba, 2,4-D, picloram, and commercially available premixes of glyphosate plus 2,4-D or glyphosate plus dicamba were evaluated alone and in combination for field bindweed control in a winter wheat-fallow system in Colorado, Wyoming, Kansas, and Montana. Approximately one year after application, herbicide mixtures containing picloram at 0.14 or 0.28 kg ai ha-1 provided the best control. In five of seven locations, the control provided by picloram in herbicide mixtures was greater than the control provided by glyphosate plus 2,4-D, 2,4-D, or dicamba when these products were mixed with picloram. Glyphosate plus 2,4-D or glyphosate plus dicamba premixes, or 2,4-D added to dicamba were less effective for long-term control of field bindweed than mixtures containing 0.14 kg ai ha-1 or more of picloram. Under drought conditions in Kansas in 1988, picloram did not control field bindweed as well as in Colorado, Wyoming, or Montana where rainfall was normal.

A rhizotron containing 10 1.5-by 1.2-by 2.4-m chambers and two 1.5-by 1.2-by 1.2-m chambers for plant growth was constructed for teaching purposes at Montana State University. The rhizotron, constructed of pressure-treated plywood and redwood lumber, has a life expectancy of 40 yr and a prorated cost of approximately $100/yr.

Field observations indicate that sulfonylurea-resistant kochia may germinate at lower soil temperatures and/or germinate more rapidly than susceptible kochia in the absence of herbicide. To investigate this possibility, seeds from three resistant and two susceptible kochia accessions were germinated at temperatures ranging from 4.6 to 13.2 C on thermal gradient plates. At 4.6 and 13.2 C, germination rates of all resistant accessions were higher than susceptible accessions, while germination rates of one resistant accession were higher than susceptible accessions at 7.2 and 10.5 C. Percent germination of all resistant accessions was significantly higher than susceptible accessions after 48 h at 4.6 C. At higher temperatures, percent germination of some resistant accessions was higher after 12 or 24 h, but germination of all accessions was similar at later times. HPLC analysis revealed that seeds from resistant accessions contained about 2-fold higher free levels of branched chain amino acids than seeds from susceptible accessions. The results indicate that mutations conferring resistance to sulfonylurea herbicides in these kochia accessions may concomitantly reduce or abolish acetolactate synthase sensitivity to normal feedback inhibition patterns, resulting in elevated levels of branched chain amino acids available for cell division and growth during early germination.

Soil characteristics, elevation, annual precipitation, potential evapotranspiration, length of frost-free season, and mean maximum July temperature were estimated for 116 established infestations of spotted knapweed (Centaurea maculosa Lam. # CENMA) in Montana using basic land resource maps. Areas potentially vulnerable to invasion by the plant were delineated on the basis of representative edaphic and climatic characteristics. No single environmental variable was an effective predictor of sites vulnerable to invasion by spotted knapweed. Only a combination of variables was effective, indicating that the factors that regulate adaptability of this plant are complex. This technique provides a first approximation map of the regions most similar environmentally to infested sites and, therefore, most vulnerable to further invasion. This weed migration prediction technique shows promise for predicting suitable habitats of other invader species.

Experiments were established in 1982 to study the loss of viability of spotted knapweed seeds (achenes) in soil. Greater than 50 and 25% of buried seeds remained viable but dormant after 5 and 8 yr at two locations, respectively. Decline in seed viability was also measured in two natural seedbanks. Although the soil seed reserve decreased by 95% over a 7-yr period, approximately 400 000 viable seeds per ha remained, indicating that spotted knapweed seeds will last for many years in soil.

Repeated use of the preemergence herbicide triallate has selected for wild oat populations that are resistant (R) to field use rates. Field collections and an inbred R line were shown in greenhouse and petri dish dose response experiments to be 6- to 20-fold more tolerant to triallate than susceptible (S) lines. R populations and the inbred line were also resistant (8-fold) to the related thiocarbamate herbicide diallate, as well as to the chemically unrelated postemergence herbicide difenzoquat (60-fold). 14C-triallate uptake and translocation patterns were similar between R and S lines for the first 24 h after application. However, translocation of radioactivity was more rapid in S lines than R lines from 24 through 60 h after application. 14C-difenzoquat uptake was the same in R and S lines 12 h after application, but was 10 to 20% higher in R lines than S lines by 24 through 96 h after application. Similarly, translocation of radioactivity after 14C-difenzoquat application was 7 to 14% greater in R than S lines after 12 h, although translocated radioactivity amounts were not significantly different between R and S lines. The relatively minor differences in triallate and difenzoquat uptake and translocation patterns between R and S lines are most likely not of sufficient magnitude to explain the observed resistance levels.

Larvae of the leafy spurge hawk moth were sprayed with and/or were fed leafy spurge plants sprayed with 2,4-D or picloram. Larval survival was not affected from contact with the chemicals, from feeding on contaminated foliage, or from a combination of both. Viable second-generation adult moths were produced in all treatments. Thus, leafy spurge infested with the leafy spurge hawk moth can be sprayed with 2,4-D or picloram without injury to larvae that have reached the fourth or fifth instar stages.

AC 222,293, a postemergence herbicide for wild oat control in wheat, barley, and sunflower, was applied at five rates at three locations in Montana in the spring of 1985. Twelve and 11 crops were seeded into treated soil in 1985 and 1986, respectively. Sugarbeet, lentil, and rape were injured by residual levels of AC 222,293 14 months after application. Injury was significantly greater at one location, indicating herbicide bioavailability varied by location. Soil pH was correlated negatively with crop injury, indicating the herbicide persists longer on lower pH soils.

Puccinia obtegens (Link) Tul., an autoecious rust pathogen, is a potential biological control agent of Canada thistle [Cirsium arvense (L.) Scop.]. Ten ecotypes of Canada thistle were inoculated with uredospores of P. obtegens and sporulation was observed on all ecotypes. Infection types varied among and within ecotypes, indicating that host-resistance is one factor limiting rust infection. No correlation was found between Canada thistle susceptibility to the rust and host plant ecotype classification, stomatal density, amount of leaf pubescence, or spore germinability on leaf surfaces.

Kochia pollen dispersion was measured during 24 and 48 h periods from a kochia population in an 8- by 10-m area in the center of a 1.6 ha fallow field. Pollen counts from traps at 50- and 100-cm heights declined rapidly with increasing distance from the pollen source. Pollen deposition was highest along the prevailing wind direction: up to 23 pollen grains cm–2 were recovered 50 m from the pollen source along the southeast (SE) vector. Nonlinear regression analysis of pollen deposition along the SE vector was used to estimate that 99.9% of shed pollen would be deposited within 154.4 m of the source. Viability of pollen from greenhouse- and field-grown plants was measured using staining and germination assays. of four pollen stains tested, only 1,2,3-triphenyl tetrazolium chloride gave consistent results and did not stain heat-killed pollen. Depending on environmental conditions, kochia pollen remained viable from less than 1 d to 12 d. Length of kochia pollen viability was shortest under high temperatures (22 and 28 C) and low relative humidity (7 and 32%). Less than 0.5% germination was observed in 1.1% agar media with various additions; however, up to 17.8% germination was observed after incubation at 28 C in 100% relative humidity.

The forage selection and grazing behavior of sheep in leafy spurge (Euphorbia esula L. ♯3 EPHES)-infested pastures was examined in a 3-month grazing study conducted during the summer, 1981. No definite preference for or avoidance of leafy spurge was detected. An initial 1- to 3-week avoidance was observed, followed by a steady increase in leafy spurge consumption. A maximum intake of 40 to 50% of the diet per animal was observed. Weight gain by ewes in pastures infested with leafy spurge was not significantly different from weight gain by ewes in pastures free of leafy spurge. Results from these studies indicate that leafy spurge may be classified as a forage for sheep. Sheep will consume a significant amount of vegetation while suffering neither harmful internal effects nor a significant loss of body weight. Sheep can be considered an effective biological control agent for leafy spurge.

Clomazone is effective as a chemical fallow herbicide; however, its soil residual properties in the Northern Great Plains are unknown. Clomazone was applied to soil at 0.6, 1.1, and 2.2 kg ai/ha at two locations in Montana in the spring of 1986. Soil samples were taken at each location at monthly intervals for 6 months. Residual levels of the herbicide were estimated by measuring the percent chlorosis by height in oat leaves. Clomazone at 2.2 kg ai/ha applied to a loam soil dissipated to levels below 0.1 mg/kg in 3 months and applied to a silty clay loam soil dissipated to 0.2 mg/kg 6 months after application. Half-lives (t½), determined from first-order rate plots, were 33 and 37 days in the Willow Creek loam and Bozeman silty clay loam, respectively. Thus, clomazone residue from labeled-use rates should not inhibit wheat in a wheat-fallow-wheat cropping system in Montana.

The methods used to teach weed identification at 20 U.S. universities were obtained for comparison through a telephone survey in December, 1986, and January, 1987. Weed identification is taught as a portion (30%) of the laboratory section in introductory weed science courses. Only five have a separate weed identification course. Field trips frequently are used to teach weed identification. Students must learn from 50 to 125 weed species with some seedling identification. Pressed plant collections of approximately 50 weed species normally are required. Most instructors strongly suggest using live plants and repetition for long-term learning.

Glyphosate [N-(phosphonomethyl)glycine] was applied in the spring and fall to leafy spurge (Euphorbia esula L. # EPHES) in the field at rates of 0.14 to 4.48 kg ai/ha. Fall applications of glyphosate at rates of 0.56 to 4.48 kg/ha stimulated axillary branching and caused an increase in the number of stems/m2 by the end of the following summer in a dense population. This was a result of shoot growth from buds on the crown region of the root system. The absorption and translocation of 14C-glyphosate applied to leaves of mature leafy spurge plants were evaluated at prebloom, full-bloom, and senescence phenological stages. Approximately 81% of the 14C-glyphosate applied to the leaves of senescing plants was absorbed. There was a decrease in the proportion of 14C translocated out of the treated leaf when applications were made after full bloom. Translocation of 14C to the treated stem, nontreated stems, root crown, and roots did not differ with phenological stage in 1983. Translocation and concentration of 14C in most plant parts in 1985 differed with phenological stage. Translocation to the crown buds as a percentage of the 14C absorbed was highest at the senescence stage. At senescence and before soil freezing, leafy spurge crown buds demonstrate transient but active elongation toward the soil surface. This active development may account for the enhanced translocation of 14C into the crown buds.

The potential of mourning doves (Zenaida macroura L.) as dispersers of leafy spurge (Euphorbia esula L. # EPHES) seeds was investigated by examing digestive tract contents and feces. Very few intact seeds passed through the digestive systems of wild or captive adult mourning doves. An average of less than one intact leafy spurge seed/g was recovered from fecal material found in dove nests. These results suggest that adult mourning doves are primarily consumers of leafy spurge seeds and rarely act as dispersal agents. It appears that dispersal is only possible when seeds passing through the digestive tracts of nestlings are deposited in the nest and the nest later falls.

Oxeye daisy has invaded seeded pastures, roadsides, and mountain rangelands in western Montana. In 1990, we began a study to: (1) determine use of oxeye daisy and introduced perennial grasses by cattle; (2) determine effects of intensive cattle grazing on the number of oxeye daisy seeds in the soil; and (3) assess effects of intensive grazing on year-to-year changes in oxeye daisy and associated perennial grasses. Cattle grazed oxeye daisy but much of their impact was from trampling or removing stems. The number of oxeye daisy seeds in the soil seedbank was lower in 1992 than in 1990 in grazed areas, whereas the number was higher in ungrazed areas. Two years of intensive grazing reduced densities of oxeye daisy seedlings and rosettes, but did not change densities of mature stems. Intensive grazing had minimal impact on the introduced grasses.