OBJECTIVES/GOALS: 1. Describe method of Community Based System Dynamics. 2. Describe CBSD as used in addressing structural racism in a previously redlined community. 3. Review example causal loop diagrams and simulation models developed using CBSD focused on structural racism as a social determinant of health. METHODS/STUDY POPULATION: Community Based System Dynamics (group model building and computer simulation development). Cuyahoga County, Ohio, a previously redlined community of more than 1.2 million people. RESULTS/ANTICIPATED RESULTS: Actionable Community identified leverage points for action to mitigate structural racism. Computer simulation models built on causal loop diagrams built by Community members with loved experience. DISCUSSION/SIGNIFICANCE: Structural and social determinants of health, such as racism, have profound impacts on the health of individuals and populations, however they remain challenging to address in pragmatic ways. CBSD is a novel method to engage community members not proximal to the impact of structural racism in generating maps of the complex, dynamic system they live in.

Precision Medicine/Health

Long-chain omega-3 polyunsaturated fatty acid (LC n-3 PUFA) supplements, rich in eicosapentaenoic acid and/or docosahexaenoic acid, are increasingly being recommended within athletic institutions. However, the wide range of doses, durations and study designs implemented across trials makes it difficult to provide clear recommendations. The importance of study design characteristics in LC n-3 PUFA trials has been detailed in cardiovascular disease research, and these considerations may guide LC n-3 PUFA study design in healthy cohorts. This systematic review examined the quality of studies and study design considerations used in evaluating the evidence for LC n-3 PUFA improving performance in physically trained adults. SCOPUS, PubMed and Web of Science electronic databases were searched to identify studies that supplemented LC n-3 PUFA in physically trained participants. Forty-six (n = 46) studies met inclusion. Most studies used a randomised control design. Risk of bias, assessed using the design-appropriate Cochrane Collaboration tool, revealed that studies had a predominant judgment of ‘some concerns’, ‘high risk’ or ‘moderate risk’ in randomised controlled, randomised crossover or non-randomised studies, respectively. A custom five-point quality assessment scale demonstrated that no study satisfied all recommendations for LC n-3 PUFA study design. This review has highlighted that the disparate range of study designs is likely contributing to the inconclusive state of outcomes pertaining to LC n-3 PUFA as a potential ergogenic aid. Further research must adequately account for the specific LC n-3 PUFA study design considerations, underpinned by a clear hypothesis, to achieve evidence-based dose, duration and composition recommendations for physically trained individuals.

The insurrection at the United States Capitol on January 6, 2021, was the most dramatic contemporary manifestation of deep political polarization in the United States. Recent research shows that violent protests shape political behavior and attachments, but several questions remain unanswered. Using day-level panel data from a large sample of US social media users to track changes in the identities expressed in their Twitter biographies, we show that the Capitol insurrection caused a large-scale decrease in outward expressions of identification with the Republican Party and Donald Trump, with no indication of reidentification in the weeks that followed. This finding suggests that there are limits to party loyalty: a violent attack on democratic institutions sets boundaries on partisanship, even among avowed partisans. Furthermore, the finding that political violence can deflect copartisans carries the potential positive democratic implication that those who encourage or associate themselves with such violence pay a political cost.

Ips typographus (L.) and Pityogenes chalcographus (L.) (Coleoptera: Curculionidae) are two common bark beetle species on Norway spruce in Eurasia. Multiple biotic and abiotic factors affect the life cycles of these two beetles, shaping their ecology and evolution. In this article, we provide a comprehensive and comparative summary of selected life-history traits. We highlight similarities and differences in biotic factors, like host range, interspecific competition, host colonization, reproductive behaviour and fungal symbioses. Moreover, we focus on the species' responses to abiotic factors and compare their temperature-dependent development and flight behaviour, cold adaptations and diapause strategies. Differences in biotic and abiotic traits might be the result of recent, species-specific evolutionary histories, particularly during the Pleistocene, with differences in glacial survival and postglacial recolonization. Finally, we discuss future research directions to understand ecological and evolutionary pathways of the two bark beetle species, for both basic research and applied forest management.

The southern United States produces 90% of the nation’s cotton, and the Texas High Plains is the largest contiguous cotton producing region. Since 2011, glyphosate-resistant Palmer amaranth has complicated cotton production, and alternatives to glyphosate are needed. Integrating soil residual herbicides into a weed management program is a crucial step to control glyphosate resistant weeds before emergence. The recent development of p-hydroxyphenylpyruvate dioxygenase (HPPD)-resistant cotton by BASF Corporation may allow growers to use isoxaflutole in future weed management programs. In 2019 and 2020, field experiments were conducted in New Deal, Lubbock, and Halfway, Texas, to evaluate HPPD-resistant cotton response to isoxaflutole applied preemergence (PRE) or early postemergence (EPOST) and to determine the efficacy of isoxaflutole when used as part of a season-long weed management program. At the New Deal location, cotton response was observed following the EPOST application, but it never exceeded 10%. Cotton response was greatest following the PRE application in Lubbock in 2019 but did not exceed 14%. In 2020 in Lubbock, cotton was replanted due to severe weather. There was <1% cotton response following the PRE application, and maximum cotton response observed was 9% following EPOST and mid-postemergence (MPOST) applications. Cotton lint yields were not different from those of the nontreated, weed-free control at either location. In non-crop weed control studies in Halfway, all treatments controlled Palmer amaranth ≥94% 21 d after the EPOST application. Twenty-one days after the MPOST treatment, systems with isoxaflutole applied EPOST controlled Palmer amaranth by 88% to 93%, while systems with isoxaflutole PRE controlled Palmer amaranth by 94% to 98%. End-of-season Palmer amaranth control was lowest in the system without isoxaflutole (88%) and when isoxaflutole was used EPOST (88% to 91%). These studies suggest that the use of isoxaflutole in cotton weed management systems may improve season-long control of several troublesome weeds with no adverse effects on cotton yield and quality.