The indiscriminate use of antibiotics to increase animal production may lead to residues in animal products, what justifies the monitoring of those chemicals (Heitzman, 1996). The intensification of livestock production in the world has been observed with an increasing use of drugs and growing promoter substances used as animal treatment or disease prevention. Several analytic techniques have been used to detect antibiotics residues of animal products such as fluorometry, liquid-gas chromatography, high performance liquid chromatography and immunological methods. The use of ELISA (“enzyme-linked-immunosorbent assays”) combined to specific antibodies has been employed on routine clinic diagnosis, identifying and quantifying species and substances. In this study, the objective was the production of polyclonal antibody to detect the norfloxacin antibiotic by indirect ELISA method.