To send content items to your account,
please confirm that you agree to abide by our usage policies.
If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account.
Find out more about sending content to .
To send content items to your Kindle, first ensure firstname.lastname@example.org
is added to your Approved Personal Document E-mail List under your Personal Document Settings
on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part
of your Kindle email address below.
Find out more about sending to your Kindle.
Note you can select to send to either the @free.kindle.com or @kindle.com variations.
‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi.
‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.
This chapter considers the evolution of the practical application of cryopreservation technology to achieve consistently acceptable levels of cryosurvival of this highly cryosensitive gamete while retaining its inherent competency. The majority of oocyte freezing applied clinically has been based directly on traditional human embryo cryopreservation protocols. Such protocols utilize a "slow-freeze/rapid-thaw" approach necessitating use of a programmable freezer, and these protocols have produced to date the majority of the approximately 900 offspring worldwide. With regard to vitrification as a means to cryopreserve oocytes, increased speed of cooling, through the use of better designed carriers and protocols that lessen the concentration of cryoprotectant used while hastening exposure and procedure times, has put this technology on the map, and excellent embryo quality can be obtained from vitrified oocytes. To date vitrification as a cryopreservation method has had relatively little practical impact on human assisted reproduction.