Although dandelion has been recognized for some time as a common weed of perennial plant stands, recent weed surveys in western Canada indicate that dandelion has become more common in fields where annual spring crops are grown. Because little has been published on dandelion control in annual crops, a field study was conducted at two locations in southern Manitoba in 1999 and 2000 investigating the effect of spring tillage, glyphosate dosage, and application timing on dandelion control in a spring annual glyphosate-resistant canola crop. The experiments were situated in areas known to have natural infestations of dandelion. Final assessments of dandelion control were performed the spring following treatment, i.e., the next year, to provide a better indication of treatment efficacy on this perennial weed. Spring tillage alone did not significantly reduce dandelion density, as assessed the following spring, but did reduce dandelion shoot dry matter at three of the five site-years by up to 84%. Glyphosate was applied preplant, in-crop, and postharvest at dosages ranging from 450 g ae/ha in-crop to 2,700 g ae/ha postharvest. Glyphosate application after the canola crop had been harvested provided the greatest level of dandelion control, with a single postharvest application of 900 g ae/ha of glyphosate reducing dandelion density and shoot dry matter by 88 and 96%, respectively, the following spring. Applications of glyphosate either preplant or in-crop were not nearly as effective as the postharvest treatments in reducing dandelion density and shoot dry matter the following spring.