Mitotic-inhibiting herbicides, like prodiamine and dithiopyr, are used to control annual bluegrass (Poa annua L.) preemergence in managed turfgrass; however, resistance to mitotic-inhibiting herbicides has evolved due to repeated applications of herbicide from a single mechanism of action. Three suspected resistant populations (R1, R2, and R3) were collected in Alabama and Florida and screened for resistance to prodiamine. Part of the α-tubulin gene was sequenced for known target-site mutations. Target-site mutations were reported in all three R populations, with each containing an amino acid substitution at position 239 from threonine to isoleucine (Thr-239-Ile). Previous research has indicated that the Thr-239-Ile mutation confers resistance to dinitroaniline herbicides in other species. Dose–response screens using prodiamine and dithiopyr were conducted and I50 values were calculated for R1, R2, and R3 using regression models based on seedling emergence. For prodiamine, I50 values for R1, R2, and R3 were 35.3, 502.7, and 91.5 g ai ha−1, respectively, resulting in 2.9-, 41.9-, and 7.6-fold resistance, respectively, when compared with a susceptible (S) population. For dithiopyr, I50 values for R1, R2, and R3 were 154.0, 114.2, and 190.1 g ai ha−1, respectively, resulting in 3.6-, 2.7-, and 4.5-fold resistance, respectively, when compared with an S population. When comparing I90 values with the highest labeled use rates, R2 had a 2.9-fold level of resistance to prodiamine, and R1, R2, and R3 had a 2.4-, 2.0-, and 3.2-fold levels of resistance to dithiopyr, respectively. This is the first report of a variable response in P. annua to prodiamine despite each R population possessing the same mutation.

Dithiopyr and dinitroanilines are preemergence-applied, mitotic-inhibiting herbicides used to control goosegrass [Eleusine indica (L.) Gaertn.] in turfgrass. A suspected resistant E. indica population was collected from a golf course putting green and was evaluated for possible resistance to dithiopyr and prodiamine. After dose–response evaluation, the α-tubulin gene was sequenced for known target-site mutations that have been reported to confer resistance to mitotic-inhibiting herbicides. A mutation was discovered that resulted in an amino acid substitution at position 136 from leucine to phenylalanine (Leu-136-Phe). Previous research has indicated that Leu-136-Phe does confer resistance to dinitroaniline herbicides. The level of resistance indicated by regression models and I50 values indicates that there is 54.1-, 4.7-, >100-, and >100-fold resistance to dithiopyr, prodiamine, pendimethalin, and oryzalin, respectively, when compared with the susceptible population based on seedling emergence response and 88.4-, 7.8-, >100-, and >100-fold resistance to dithiopyr, prodiamine, pendimethalin, and oryzalin, respectively, when compared with the susceptible population based on biomass reduction response. This is the first report of less resistance to prodiamine compared with pendimethalin or oryzalin due to a target-site α-tubulin mutation and the first report of a target-site α-tubulin mutation associated with dithiopyr resistance.

The redshifted 21cm line of neutral hydrogen (Hi), potentially observable at low radio frequencies (~50–200 MHz), should be a powerful probe of the physical conditions of the inter-galactic medium during Cosmic Dawn and the Epoch of Reionisation (EoR). The sky-averaged Hi signal is expected to be extremely weak (~100 mK) in comparison to the foreground of up to 104 K at the lowest frequencies of interest. The detection of such a weak signal requires an extremely stable, well characterised system and a good understanding of the foregrounds. Development of a nearly perfectly (~mK accuracy) calibrated total power radiometer system is essential for this type of experiment. We present the BIGHORNS (Broadband Instrument for Global HydrOgen ReioNisation Signal) experiment which was designed and built to detect the sky-averaged Hi signal from the EoR at low radio frequencies. The BIGHORNS system is a mobile total power radiometer, which can be deployed in any remote location in order to collect radio frequency interference (RFI) free data. The system was deployed in remote, radio quiet locations in Western Australia and low RFI sky data have been collected. We present a description of the system, its characteristics, details of data analysis, and calibration. We have identified multiple challenges to achieving the required measurement precision, which triggered two major improvements for the future system.

In this paper, we developed and compared several expectation–maximization (EM) algorithms to find maximum likelihood estimates of individual inbreeding coefficients using molecular marker information. The first method estimates the inbreeding coefficient for a single individual and assumes that allele frequencies are known without error. The second method jointly estimates inbreeding coefficients and allele frequencies for a set of individuals that have been genotyped at several loci. The third method generalizes the second method to include the case in which null alleles may be present. In particular, it is able to jointly estimate individual inbreeding coefficients and allele frequencies, including the frequencies of null alleles, and accounts for missing data. We compared our methods with several other estimation procedures using simulated data and found that our methods perform well. The maximum likelihood estimators consistently gave among the lowest root-mean-square-error (RMSE) of all the estimators that were compared. Our estimator that accounts for null alleles performed particularly well and was able to tease apart the effects of null alleles, randomly missing genotypes and differing degrees of inbreeding among members of the datasets we analysed. To illustrate the performance of our estimators, we analysed previously published datasets on mice (Mus musculus) and white-tailed deer (Odocoileus virginianus).

Improved accuracy in measurement of the gravitational time delay of electromagnetic waves passing by the sun may be achieved with two drag-free spacecraft, one with a stable clock and laser transmitter and one with a high-stability transponder. We consider one spacecraft near the Earth-Sun L1 point with an advanced optical clock, and the transponder on a second satellite, which has a 2 year period orbit and eccentricity e = 0.37. Superior conjunctions will occur at aphelion 1, 3, and 5 years after launch of the second spacecraft. The measurements can be made using carrier phase comparisons on the laser beam that would be sent to the distant spacecraft and then transponded back. Recent development of clocks based on optical transitions in cooled and trapped ions or atoms indicate that a noise spectral amplitude of about 5 × 10−15/ at frequencies down to at least 1 microhertz can be achieved in space-borne clocks. An attractive candidate is a clock based on a single laser-cooled Yb+ trapped ion. Both spacecraft can be drag-free at a level of 1×10−13m/s2/ at frequencies down to at least 1 microhertz. The corresponding requirement for the LISA gravitational wave mission is 3 × 10−15m/s2/ at frequencies down to 10−4 Hz, and Gravitational Reference Sensors have been developed to meet this goal. They will be tested in the LISA Pathfinder mission, planned by ESA for flight in 2011. The requirements to extend the performance to longer times are mainly thermal. The achievable accuracy for determining the PPN parameter γ is about 1 × 10−8.

Reaction zone phases and kinetics over a temperature range of 650-1200°C have been characterized for Ti-14Al-21Nb/SiC and Ti-6Al-2Sn-4Zr-2Mo/SiC fibrous composites. The matrix of the Ti-1421/SiC materials was α2 with varying amounts of beta phase and transformed beta phase, and the Ti-6242 matrix consisted of alpha plus beta. The reaction zone in the as-HIP Ti-1421/SiC contained fine-grained TiC, a region of coarser-grained TiC, a carbide layer containing Ti, Al and Nb, and a region of(Ti, Nb)5(Si, Al)3. The matrix adjacent to the reaction zone was α2-Ti3Al with no beta phase. In the as-HIP Ti-6242/SiC composites, the reaction zone contained TiC near the fiber, and a layer of (Ti,Zr)5Si3 adjacent to the matrix. In heat-treated samples, the reaction zone thicknesses varied in a linear fashion with the square-root of aging time, indicating diffusion-controlled growth. Incubation periods for reaction zone growth were observed in Ti-1421/SiC samples aged at 760-1000°C. The overall kinetics for the two matrix alloys were approximately the same, and the activation energy was measured as 63-73 kcal/mole for Ti-1421/SiC and 73kcal/mole for Ti-6242/SiC.

The quality of the bond produced after mating oxidized and/or unoxidized silicon wafers has been studied using acoustic microscopy, infrared transmission thermographs, and transmission electron microscopy. The acoustic microscopy revealed that a significant number of unbonded regions (gaps) remain at the bond interface after bonding in oxygen, nitrogen, or high vacuum, and then annealing. These gaps could be virtually eliminated by a subsequent hyperbaric annealing step. The thermal imaging was found to have insufficient resolution to give a detailed picture of the bond quality. Transmission electron microscopy showed that an excellent bond could be produced when bonding clean silicon wafers, with only very small oxide or void bubbles present at the interface. Bonding two oxidized wafers resulted in a buried oxide layer with no detectable bond line. Mating an oxidized wafer to an unoxidized wafer produced a bonded silicon/oxide interface which was nearly indistiguishable from the wafer/thermal oxide interface.