The recent evidence of a peculiar distribution of X-ray sources in the elliptical galaxy NGC 4261 reported by Zezas et al. has prompted us to study this galaxy combining archive X-ray and optical observations, from Chandra, INT, and HST. We find that a sizable fraction of the X-ray sources has a globular cluster as optical counterpart. This together with the shape of the luminosity function of the X-ray sources suggest that they are accreting low-mass binaries. We further show a remarkable similarity in the anisotropy of the projected spatial distributions of the optical and X-ray sources, which leads us to conclude that the spatial anisotropy of the X-ray sources in NGC 4261 is due to the anisotropy of the globular cluster population.