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3 results

  • Hold-'Em In Place

  • Kristen R. Ross, Michael E. Berens
  • Journal:
    Microscopy Today / Volume 9 / Issue 2 / March 2001
    Published online by Cambridge University Press:
    14 March 2018, pp. 18-21
    Print publication:
    March 2001
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  • We've used gelled agarose to cover & hold monolayer cells in place when treatment leads to cell lifting. Low-gelling temperature agarose (Type VII) (Sigma A 4018 or A 0701) can be made as a 2% stock solution in “phosphate-buffered saline” (PBS) (boiled then kept at 42° C to remain liquid). The agarose can be stored at room temperature at this time, but must be boiled and melted again before use.

  • Home-Made Cell Tack

  • Kristen R. Ross, Micheal E. Berens
  • Journal:
    Microscopy Today / Volume 8 / Issue 8 / October 2000
    Published online by Cambridge University Press:
    14 March 2018, p. 36
    Print publication:
    October 2000
    • Article
      • You have access
    • PDF
    • Export citation

  • We've used gelled agarose to cover & hold monolayer cells in place when treatment leads to cell lifting. Low-gelling temperature agarose (Type VII) (Sigma A 4018 or A 0701) can be made as a 2% stock solution in “phosphate-buffered saline” (PBS) (boiled then kept at 42° C to remain liquid).