In order to clarify and visualize the real state of the structural performances of ships in operation and establish a more optimal, data-driven framework for ship design, construction and operation, an industry-academia joint R&D project on the digital twin for ship structures (DTSS) was conducted in Japan. This paper presents the major achievements of the project. The DTSS aims to grasp the stress responses over the whole ship structure in waves by data assimilation that merges hull monitoring and numerical simulation. Three data assimilation methods, namely, the wave spectrum method, Kalman filter method, and inverse finite element method were used, and their effectiveness was examined through model and full-scale ship measurements. Methods for predicting short-term extreme responses and long-term cumulative fatigue damage were developed for navigation and maintenance support using statistical approaches. In comparison with conventional approaches, response predictions were significantly improved by DTSS using real response data in encountered waves. Utilization scenarios for DTSS in the maritime industry were presented from the viewpoints of navigation support, maintenance support, rule improvement, and product value improvement, together with future research needs for implementation in the maritime industry.

Various factors such as embryo density and substances in the medium can influence embryo development in vitro. These factors and the embryos probably interact with each other, however the interactions are not fully understood. To investigate the interactions, we examined the effects of the number of embryos, drop size, oxygen concentration and glucose and inorganic phosphate in the medium during protein-free culture of bovine IVM/IVF embryos. In Experiment 1, different numbers of embryos were cultured in a 50 μl drop of medium. The frequencies of blastocyst development in the groups with 25, 50 and 100 embryos per drop were higher than in the other groups. One, five and 25 embryos were cultured in different drop sizes (Experiment 2), a 50 µl drop of medium at different O2 concentrations (Experiment 3) and a 50 µl drop of medium excluding glucose and/or inorganic phosphate (Experiment 4). In Experiment 2, the size of the medium drops did not improve blastocyst development. In Experiment 3, the highest frequency of blastocyst development for one, five and 25 embryos per drop was obtained at 1, 2.5 and 5% O2, respectively. In Experiment 4, blastocyst development for one and five embryos per drop were improved in the medium excluded inorganic phosphate. These results indicate that there is a cooperative interaction among embryos during culture and that this interaction may be mediated by reduction of toxic factors in the medium. At low embryo density, reduced oxygen concentration or the exclusion of inorganic phosphate enhanced blastocyst development.

An early stage of diamond growth in hot-filament chemical vapor deposition on silicon substrates was examined by the high resolution electron microscope. “Pretreatment” of the substrate surfaces by diamond powder abrading was found to plant diamond seed crystals with a density of as high as 1011/cm2. These crystals provide sites for subsequent growth of diamond films. The CVD grown diamond particles tend to be cuboctahedra. Smaller particles in nanometer size are faultless, but larger ones of several tens of nanometers develop crystal faults. Some of them may originate from the seed crystals. Degradation of the diamond seed crystals due to the electron beam irradiation is discussed in terms of fabrication of diamond film.