Early in the coronavirus disease 2019 (COVID-19) pandemic, the CDC recommended collection of a lower respiratory tract (LRT) specimen for severe acute respiratory coronavirus virus 2 (SARS-CoV-2) testing in addition to the routinely recommended upper respiratory tract (URT) testing in mechanically ventilated patients. Significant operational challenges were noted at our institution using this approach. In this report, we describe our experience with routine collection of paired URT and LRT sample testing. Our results revealed a high concordance between the 2 sources, and that all children tested for SARS-CoV-2 were appropriately diagnosed with URT testing alone. There was no added benefit to LRT testing. Based on these findings, our institutional approach was therefore adjusted to sample the URT alone for most patients, with LRT sampling reserved for patients with ongoing clinical suspicion for SARS-CoV-2 after a negative URT test.

The magnetic resonance imaging (MRI) appearance of the brain and spinal cord in humans with neuroangiostrongyliasis (NA) due to Angiostrongylus cantonensis infection has been well reported. Equivalent studies in animals are lacking. This case series describes clinical and MRI findings in 11 dogs with presumptively or definitively diagnosed NA. MRI of the brain and/or spinal cord was performed using high-field (1.5 T) or low-field (0.25 T) scanners using various combinations of transverse, sagittal, dorsal and three-dimensional (3D) T1-weighted (T1W), transverse, sagittal and dorsal T2-weighted (T2W), T2W fluid-attenuated inversion recovery (FLAIR) and T2*-weighted (T2*W) gradient echo (GRE), dorsal T2W short tau inversion recovery (STIR) and post-gadolinium transverse, sagittal, dorsal and 3D T1W and transverse T2W FLAIR sequences. In 4/6 cases where the brain was imaged, changes consistent with diffuse meningoencephalitis were observed. Evidence of meningeal involvement was evident even when not clinically apparent. The spinal cord was imaged in 9 dogs, with evidence of meningitis and myelitis detected in regions consistent with the observed neuroanatomical localization. Pathognomonic changes of neural larva migrans, as described in some human patients with NA, were not detected. NA should be considered in the differential diagnosis of dogs with MRI evidence of focal or diffuse meningitis, myelitis and/or encephalitis, especially in areas where A. cantonensis is endemic. If not precluded by imaging findings suggestive of brain herniation, cerebrospinal fluid (CSF) collection for cytology, fluid analysis, real-time polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA) testing should be considered mandatory in such cases after the MRI studies.

We examine the environmental impacts of a cash transfer program in rural Zambia and investigate whether variation in market access is associated with heterogeneous impacts on natural resource use. We consider households’ use of firewood, charcoal, bushmeat and land for farming, as well as their ownership of non-farm businesses. We find that cash transfers increase the likelihood of charcoal consumption as well as the amount consumed for those living close to paved roads. The transfers also enable households to increase the size of their farms and establish non-farm businesses. These impacts are most pronounced for those living far from paved roads. While remoteness is associated with farm expansion in response to the cash transfer, more education causes those receiving the transfer to decrease the size of their farms. This impact heterogeneity has important implications for sustainable development.

It has been proposed that cropping systems can be managed to promote the development of soil microbial communities that accelerate weed seed mortality. We examined soil fungal and bacterial communities, soil C:N ratio, soil particle size fractions, and weed seed mortality in soil from fields with over 10 yr of five contrasting management histories with the objective of determining if seed mortality could be explained by differences in soil properties. Seed mortality of giant foxtail and velvetleaf were greatest in soil from the conventionally managed systems and lowest in soil from a reduced input system. Principal-components analysis of soil microbial communities, as determined through denaturing gradient gel electrophoresis of polymerase chain reaction–amplified ribosomal RNA genes (PCR-DGGE), showed distinct differences in the composition of fungal and bacterial communities among the study soils. The first principal component of the 18S rDNA PCR-DGGE analysis of fungal community composition showed a strong negative correlation with both giant foxtail (− 0.52, P < 0.05) and velvetleaf (− 0.57, P < 0.01) seed mortality, as did ordination with nonmetric multidimensional scaling (NMS) [giant foxtail (− 0.54, P < 0.01) and velvetleaf (− 0.60, P < 0.01)], suggesting that seeds of the two species were affected similarly by changes in the soil fungal community. For giant foxtail, weed seed mortality was also positively correlated (r = 0.48, P < 0.05) with the first NMS axis of the bacterial 16S rDNA analysis. None of the other measured soil properties were significantly correlated with weed seed mortality. Thus, for the soils tested here, management history, microbial community composition, and weed seed mortality were linked. To extend these results to the field, more work is needed to identify components of the fungal and bacterial communities that are active in seed degradation, and to develop conservation biocontrol recommendations for these species.

Burnout occurs among students when they suddenly lose interest in their studies due to feeling physically and emotionally drained. They experience further emotional depletion due to study demands, distrustfulness and detachment about their work. This study investigated the relationship between the Five-Factor Model (FFM) personality traits and burnout, as operationalised by Maslach's three dimensions of burnout, namely exhaustion, cynicism and reduced personal efficacy. Previous Western research was replicated in order to contribute to the cross-cultural literature on burnout. The Maslach Burnout Inventory – Student Survey University Form and the NEO – Personality Inventory Revised Form S were subjected to stepwise forward regression using FFM factors and facets to predict the scores on each burnout dimension. Five hundred and seventy-seven Filipino college students (age 17 to 24) from private universities and colleges within Metro Manila participated. Results revealed that neuroticism and conscientiousness predict all three burnout constructs. However, certain facets of neuroticism and conscientiousness are more important than others. At the facet level, facets of agreeableness and openness contributed to prediction of burnout as well.

The purpose of this pilot study was to determine whether macronutrient content (low-fat v. high-fat diet) influences an indicator of advanced glycation end products (AGE), Nε carboxymethyl-lysine (CML), in the context of a 1-d, high-AGE diet. The effect of the diets on inflammatory markers was also assessed. A total of nineteen overweight and obese adults (nine men and ten women) without known disease were recruited to participate in a crossover challenge of a high-fat, high-AGE (HFHA) and low-fat, high-AGE (LFHA) diet. In each phase patients had fasting blood drawn, followed by consumption of a high-fat or low-fat breakfast test meal, then three postprandial blood draws at 1, 2 and 3 h after consuming the test meal. After consuming high-AGE meals for the remainder of the day, participants returned the next day for a follow-up analysis. A different pattern in the 3-h post-meal CML and soluble receptor for AGE response to the two diets was observed (P=0·01 and 0·05, respectively). No change in serum CML was observed following consumption of a LFHA breakfast (535 (25th–75th percentile 451–790) to 495 (25th–75th percentile 391–682) ng/ml; P=0·36), whereas a rise in CML occurred after the HFHA breakfast (463 (25th–75th percentile 428–664) to 578 (25th–75th percentile 474–865) ng/ml; P=0·05). High sensitivity C-reactive protein and high molecular weight adiponectin were not affected by either diet. These findings suggest that dietary CML may not be as important in influencing serum CML as other dietary factors. In addition, acute exposure to dietary CML may not influence inflammation in adults without diabetes or kidney disease. This is contrary to previous findings.

To define or describe the middle ages is to take a political stance, wittingly or not. Controversy accompanies any period definition, of course, as recent skirmishes over the early modern, the modern, and modernity attest. But the politics of the Middle Ages has generally gone under the radar of literary and cultural critique, precisely because of the nature of its formation and its relation to the modern. In fact “the Middle Ages” is a colonial category, developed in the eighteenth and nineteenth centuries as (primarily western) Europeans worked to legitimize, classify, and make sense of colonial policies, practices, and encounters. The formation of medieval studies as a discipline, vital to the then incipient discipline of history, was also fully integrated with colonial bureaucracy and administration (Frantzen; Biddick; Dagenais and Greer; Ganim; Kabir; Davis; Davis and Altschul; Lampert-Weissig). As a form of temporal spacing, the category of the Middle Ages enabled the thought of Europe's difference from itself, thus making it possible not only to define European nations across time but also to establish a scale of comparison by which to measure others and to deny them coeval status—that is, equal standing as human beings in regard to law, trade, the capacity for self-rule, and so on.

The absence of engineering from K-12 curricula and mainstream media often causes students to refer back to historical stereotypes regarding what engineers look like and the type of work they do. Such misconceptions may prevent high school students from pursuing engineering as a field of study and increase the need for engineering educational programs [1]. Nano-Challenge is an outreach program that orients high school students to engineering through a one-year research internship. The program is held at the Center for Nanoscale Chemical, Electrical, Mechanical Manufacturing Systems (Nano-CEMMS) at the University of Illinois at Urbana-Champaign. A major focus of the program is to involve students from groups traditionally underrepresented in STEM fields and inform them about engineering earlier in their careers. An external program evaluation provides anecdotal information about the students’ experiences and gives feedback to inform program improvement.

Material science can be used to enrich secondary school curriculum and illuminate for students the connection between science and technology. Based on materials research being conducted at the University of Illinois, we have developed an interdisciplinary activity that integrates engineering with chemistry and material science.

Students investigate the behaviors of polymers by creating 3-dimensional (3-D) objects. Students can design objects that they “print” on the order of a cubic inch in about 20 minutes. The process students use to create these objects shows the application of engineering to material science in a novel and engaging way.

A photoactive chemical is initiated by the UV and blue light emitted from a data projector. This causes the formation of free radicals, which interact with molecules of a monomer and cause a polymerization reaction. The visual result of this reaction is that a liquid solidifies where students shine light. With black-and-white images, a data projector can direct the light to form any shape. This process can be easily modified to create true 3-D objects by adding another layer of the liquid to the top of the object and then shining the light again. With about 20 dollars worth of supplies from a hardware store, a simple staging device can be created to greatly simplify the process to create a 3-D printer in the classroom. Fabrication of this device can be done by students because the projector controls the x and y array of pixels; the object only needs to move in the z direction, unlike traditional rapid prototyping machines which control movement in the x, y, and z directions.

Results of integration into high school and college curriculum are discussed, and methods of integration and student perceptions of the activity are reported.