Glyphosate-resistant (GR) giant ragweed has been confirmed in Ontario, Canada. Giant ragweed is an extremely competitive weed and lack of control in soybean will lead to significant yield losses. Seed companies have developed new herbicide-resistant (HR) crop cultivars and hybrids that stack multiple HR traits. The objective of this research was to evaluate the efficacy of glyphosate and glyphosate plus dicamba tank mixes for the control of GR giant ragweed under Ontario environmental conditions in dicamba-tolerant (DT) soybean. Three field trials were established over a 2-yr period (2010 and 2011) on farms near Windsor and Belle River, ON. Treatments included glyphosate (900 g ae ha−1), dicamba (300 g ae ha−1), and dicamba (600 g ha−1) applied preplant (PP), POST, or sequentially in various combinations. Glyphosate applied PP, POST, or sequentially provided 22 to 68%, 40 to 47%, and 59 to 95% control of GR giant ragweed and reduced shoot dry weight 26 to 80%, 16 to 50%, and 72 to 98%, respectively. Glyphosate plus dicamba applied PP followed by glyphosate plus dicamba applied POST consistently provided 100% control of GR giant ragweed. DT soybean yield correlated with GR giant ragweed control. This is the first report in Canada of weed control in DT soybean, specifically for the control of GR giant ragweed. Results indicate that the use of dicamba in DT soybean will provide an effective option for the control of GR giant ragweed in Ontario.