Background: Data on antimicrobial use at the national level is crucial to establish domestic antimicrobial stewardship policies and enable medical institutions to benchmark against each other. This study aimed to analyze antimicrobial use in Korean hospitals. Methods: We investigated the antimicrobials prescribed in Korean hospitals between 2018 and 2021, using data from the Health Insurance Review and Assessment. Primary care hospitals (PCHs), secondary care hospitals (SCHs), and tertiary care hospitals (TCHs) were included in this analysis. Antimicrobials were categorized according to the Korea National Antimicrobial Use Analysis System (KONAS) classification, which is suitable for measuring antimicrobial use in Korean hospitals. Results: Out of more than 1,900 hospitals, PCHs and TCHs represented the largest and lowest percentage of hospitals, respectively. The most frequently prescribed antimicrobial in 2021 was piperacillin/β-lactamase inhibitor (9.3%) in TCHs, ceftriaxone (11.0%) in SCHs, and cefazedone (18.9%) in PCHs. Between 2018 and 2021, the most used antimicrobial class according to the KONAS classification was ‘broad-spectrum antibacterial agents predominantly used for community-acquired infections’ in TCHs and SCHs, and 'narrow spectrum beta-lactam agents' in PCH. Total consumption of antimicrobials has decreased from 951.7 to 929.9 days of therapy (DOT)/1,000 patient-days in TCHs and from 817.8 to 752.2 DOT/1,000 patient-days in SCHs during study period, but not in PCHs (from 504.3 to 527.2 DOT/1,000 patient-days). Moreover, in 2021, while use of reserve antimicrobials has decreased from 13.6 to 10.7 DOT/1,000 patient-days in TCHs and from 4.6 to 3.3 DOT/1,000 patient-days in SCHs, it has increased from 0.7 to 0.8 DOT/1,000 patient-days in PCHs. Conclusion: This study confirms that antimicrobial use differs by hospital type in Korea. Recent increases of use of antimicrobials, including reserve antimicrobials, in PCHs reflect the challenges that must be addressed.

In this article, we explore whether hierarchy and linearity conspire to affect agreement. The data come from an experimental study of honorific agreement between verbal si and coordinate subjects in Korean. We focus on computing the mismatch driven by honorifically mixed conjuncts. Unlike South Slavic gender agreement, Korean has neither Resolved Agreement nor First Conjunct Agreement. Only Last Conjunct Agreement is attested in Korean honorification. We show that honorific agreement within coordinate subjects is triggered only when the honorific verbal si appears, which is substantially different from the case with the honorific nominal nim. We also show that acceptability significantly decreases when the last conjunct of coordinate subjects is incongruous with an honorific verb. We thus argue that verbal honorific agreement with Korean coordinate subjects is sensitive to linear order, mimicking South Slavic gender agreement.

Townsend's attached-eddy hypothesis (AEH) provides a theoretical description of turbulence statistics in the logarithmic region in terms of coherent motions that are self-similar with the wall-normal distance ($y$). This hypothesis was further extended by Perry and coworkers who proposed attached-eddy models that predict the coexistence of the logarithmic law in the mean velocity and streamwise turbulence intensity as well as spectral scaling for the streamwise energy spectra. The AEH can be used to predict the statistical behaviours of wall turbulence, yet revealing such behaviours has remained an elusive task because the proposed description is established within the limits of asymptotically high Reynolds numbers. Here, we show the self-similar behaviour of turbulence motions contained within wall-attached structures of streamwise velocity fluctuations using the direct numerical simulation dataset of turbulent boundary layer, channel, and pipe flows ($Re_{\tau } \approx 1000$). The physical sizes of the identified structures are geometrically self-similar in terms of height, and the associated turbulence intensity follows the logarithmic variation in all three flows. Moreover, the corresponding two-dimensional energy spectra are aligned along a linear relationship between the streamwise and spanwise wavelengths ($\lambda _x$ and $\lambda _z$, respectively) in the large-scale range ($12y < \lambda _x <3\text{--}4\delta$), which is reminiscent of self-similarity. Consequently, one-dimensional spectra obtained by integrating the two-dimensional spectra over the self-similar range show some evidence for self-similar scaling $\lambda _x \sim \lambda _z$ and the possible existence of $k_x^{-1}$ and $k_z^{-1}$ scaling regions in a similar subrange. The present results reveal that the asymptotic behaviours can be obtained by identifying the self-similar coherent structures in canonical wall turbulence, albeit in low-Reynolds-number flows.

We examine the origin of very-large-scale motions (VLSMs) in fully developed turbulent pipe flow at friction Reynolds number, $\mathit{Re}_{\unicode[STIX]{x1D70F}}=934$, using data from a direct numerical simulation. The VLSMs and the packet-like large-scale motions (LSMs) found in this study are very similar to those found in earlier studies. Three-dimensional time-evolving instantaneous fields show that one component of the process leading to the large streamwise length of VLSMs is the concatenation of adjacent streamwise LSMs caused by the continuous elongation of LSMs due to the strain component of the mean shear. Spatial organization patterns of the VLSMs and LSMs and their properties are studied by separating auto-correlation of the streamwise velocity fluctuations into the components of the VLSM and the LSM defined by low-pass/high-pass filtering in the streamwise direction. The structures of the two-point spatial correlations of the streamwise velocity component of the VLSMs and the LSMs in the streamwise-azimuthal plane are characterized by multiple maxima and complex patterns that beg explanation in terms of patterned coherent arrangements of the LSMs. Using proper orthogonal decomposition (POD), it is found that the X-shape correlation pattern of the VLSMs results from the superposition of very long helically inclined structures and streamwise-aligned structures. Further explanation of the patterns in the correlations of the VLSMs and LSMs is provided through the study of synthetically constructed arrangements of simple hairpin packet models of the LSM. Head-to-tail alignment of the model packets along streamwise and helical directions suggested by the eigenvalues of the POD creates a pair of long roll-cells centred above the logarithmic layer, and bracketing the LSMs. These roll-cells are pure kinematic consequences of the induction within the LSM packets, but they may also serve to organize smaller packets.

The effect of large-scale motions (LSMs) on the turbulent/non-turbulent (T/NT) interface is examined in a turbulent boundary layer. Using flow fields from direct numerical simulation, the shape of the interface and near-interface statistics are evaluated conditional on the position of the LSM. The T/NT interface is identified using the vorticity magnitude and a streak detection algorithm is adopted to identify and track the LSMs. Two-point correlation and spectral analysis of variations in the interface height show that the spatial undulation of the interface is longer in streamwise wavelength than the boundary-layer thickness, and grows with the Reynolds number in a similar manner to the LSMs. The average variation in the interface height was evaluated conditional on the position of the LSMs. The result provides statistical evidence that the interface is locally modulated by the LSMs in both the streamwise and spanwise directions. The modulation is different when the coherent structure is high- versus low-speed motion: high-speed structures lead to a wedge-shaped deformation of the T/NT interface, which causes an anti-correlation between the angles of the interface and the internal shear layer. On the other hand, low-speed structures are correlated with crests in the interface. Finally, the sudden changes in turbulence statistics across the interface are in line with the changes in the population of low-speed structures, which consist of slower mean streamwise velocity and stronger turbulence than the high-speed counterparts.

Direct numerical simulation data from turbulent pipe and channel flows at $\mathit{Re}_{\unicode[STIX]{x1D70F}}\approx 930$ are used to investigate their statistical difference by focusing on large-scale motions (LSMs). The contribution to the bulk production of turbulent kinetic energy shows marked differences in the overlap and core regions. These discrepancies arise from the dominant contributions of the LSMs ($\unicode[STIX]{x1D706}_{x}>3\unicode[STIX]{x1D6FF}$) to the Reynolds shear stress in the channel flow. The spectrum of the net Reynolds shear force reveals that the LSMs accelerate the mean flow in the overlap region. The net force spectrum is further decomposed into the spectra of velocity–vorticity correlations, $\langle v\unicode[STIX]{x1D714}_{z}\rangle$ and $\langle -w\unicode[STIX]{x1D714}_{y}\rangle$, which are related to the advective vorticity transport and the change-of-scale effect, respectively. The dominance of large-scale accelerating motions (LSAMs) in the overlap region of the channel flow is due to the contribution of $\langle -w\unicode[STIX]{x1D714}_{y}\rangle$ at longer wavelengths ($\unicode[STIX]{x1D706}_{x}>3\unicode[STIX]{x1D6FF}$), The LSAMs are related to the long low-speed regions, and these regions are longer and wider in the channel flow than in the pipe flow. Due to the pipe curvature, the spanwise size of the LSMs is restricted by neighbouring LSMs and the spanwise velocity fluctuations are reduced. The contribution of $\langle -w\unicode[STIX]{x1D714}_{y}\rangle$ to the acceleration is prominent in the channel flow, leading to the dominance of the LSAMs associated with the change-of-scale effect.

Direct numerical simulation data of turbulent channel flow ($Re_{{\it\tau}}=930$) are used to investigate the statistics of long motions of streamwise velocity fluctuations ($u$), and the interaction of these structures with the near-wall disturbances, which is facilitated by their associated large-scale circulations. In the log layer, the negative-$u$ structures are organized into longer streamwise extent (${>}3{\it\delta}$) in comparison to the positive-$u$ counterparts. Near the wall, the footprint of negative-$u$ structures is relatively narrow in comparison to the footprint of positive-$u$ structures. This difference is due to the opposite spanwise motions in the vicinity of the footprints, which are either congregative or dispersive depending on the circulation of the outer roll cells. Conditional sampling of the footprints shows that the spanwise velocity fluctuations ($w$) are significantly enhanced by the dispersive motions of high-speed structures. On the other hand, the near-wall congregative motions of negative-$u$ structures generate relatively weak $w$ but intense negative-$u$ regions due, in part, to the spanwise collective migration of near-wall streaks. The concentrated near-wall regions of negative-$u$ upwell during the merging of the outer long scales – an effect that is demonstrated using statistical analysis of the merging process. This leads to a reduction of the convection speed of downstream negative-$u$ structures and thus promotes the merging with upstream ones. These top-down and bottom-up interactions enhance the spatial coherence of long negative-$u$ structures in the log region.

Direct numerical simulations were carried out to investigate the spatial features of large- and very-large-scale motions (LSMs and VLSMs) in a turbulent channel flow ($\def \xmlpi #1{}\def \mathsfbi #1{\boldsymbol {\mathsf {#1}}}\let \le =\leqslant \let \leq =\leqslant \let \ge =\geqslant \let \geq =\geqslant \def \Pr {\mathit {Pr}}\def \Fr {\mathit {Fr}}\def \Rey {\mathit {Re}}\mathit{Re}_{\tau }=930$). A streak detection method based on the streamwise velocity fluctuations was used to individually trace the cores of LSMs and VLSMs. We found that both the LSM and VLSM populations were large. Several of the wall-attached LSMs stretched toward the outer regions of the channel. The VLSMs consisted of inclined outer LSMs and near-wall streaks. The number of outer LSMs increased linearly with the streamwise length of the VLSMs. The temporal features of the low-speed streaks in the outer region revealed that growing and merging events dominated the large-scale (1–$3\delta $) structures. The VLSMs $({>}3\delta )$ were primarily created by merging events, and the statistical analysis of these events supported that the merging of large-scale upstream structures contributed to the formation of VLSMs. Because the local convection velocity is proportional to the streamwise velocity fluctuations, the streamwise-aligned structures of the positive- and negative-$u$ patches suggested a primary mechanism underlying the merging events. The alignment of the positive- and negative-$u$ structures may be an essential prerequisite for the formation of VLSMs.

Sesame (Sesamum indicum L.) is one of the oldest oil crops and is widely cultivated in Asia and Africa. The aim of this study was to assess the genetic diversity, phylogenetic relationships and population structure of 277 sesame core collection accessions collected from 15 countries in four different continents. A total of 158 alleles were detected among the sesame accessions, with the number varying from 3 to 25 alleles per locus and an average of 11.3. Polymorphism information content values ranged from 0.34 to 0.84, with an average of 0.568. These values indicated a high genetic diversity at 14 loci both among and within the populations. Of these, 44 genotype-specific alleles were identified in 12 of the 14 polymorphic simple sequence repeat markers. The core collection preserved a much higher level of genetic variation. Therefore, 10.1% was selected as the best sampling percentage from the whole collection when constructing the core collection. The 277 core collection accessions formed four robust clusters in the unweighted pair group method and the arithmetic averages (UPGMA) dendrogram, although the clustering did not indicate any clear division among the sesame accessions based on their geographical locations. Similar patterns were obtained using model-based structure analysis and country-based dendrograms, as some accessions situated geographically far apart were grouped together in the same cluster. The results of these analyses will increase our understanding of the genotype-specific alleles, genetic diversity and population structure of core collections, and the information can be used for the development of a future breeding strategy to improve sesame yield.

Direct numerical simulations (DNS) of turbulent boundary layers over isothermally heated walls were performed, and the effect of viscosity stratification on the turbulence statistics and skin friction were investigated. An empirical relation for temperature-dependent viscosity for water was adopted. Based on the free-stream temperature (30°C), two wall temperatures (70°C and 99°C) were selected. In the heated flows, the turbulence energy diminishes in the buffer layer, but increases near the wall. The reduction in turbulence kinetic energy in the buffer layer is accompanied by smaller levels of Reynolds shear stresses and, hence, weaker turbulence production. The enhanced turbulence energy near the wall is attributed to enhanced transfer of energy via additional diffusion-like terms due to the viscosity stratification. Despite the lower fluid viscosity near the wall, dissipation is also increased owing to the augmented near-wall fine-scale motion. Wall heating results in reduction in the skin-friction coefficient by up to 26 %. An evaluation of the different contributions to the skin friction demonstrates that drag reduction is primarily due to the changes in the Reynolds shear stresses across the boundary layer. Quadrant and octant analyses showed that ejections (Q2) and sweeps (Q4) are significantly reduced, a result further supported by an examination of outer vortical structures from linear stochastic estimation of the ejection events and spanwise vortices.

We investigate the size-dependent carrier dynamics and activation energy in cadmium telluride/zinc telluride (CdTe/ZnTe) quantum dots (QDs) grown on silicon (Si) substrates. Photoluminescence (PL) spectra show that the excitonic peak corresponding to transitions from the ground electronic subband to the ground heavy-hole band in CdTe/ZnTe QDs shifts to a lower energy level with increasing CdTe thickness, owing to an increase in the size of the CdTe QDs. Time-resolved PL measurements performed to study the carrier dynamics reveal a longer exciton lifetime for CdTe/ZnTe QDs with increasing CdTe thickness on account of the reduction of the exciton oscillator strength resulting from a strong built-in electric field in the larger QDs. The activation energy of the electrons confined in the CdTe/ZnTe QDs, as obtained from the temperature-dependent PL spectra, increases with increasing CdTe thickness. These results indicate that the carrier dynamics and activation energy of CdTe/ZnTe QDs are affected by the size of the CdTe QDs.

Interspecies intracytoplasmic sperm injection has been carried out to understand species-specific differences in oocyte environments and sperm components during fertilization. While sperm aster organization during cat fertilization requires a paternally derived centriole, mouse and hamster fertilization occur within the maternal centrosomal components. To address the questions of where sperm aster assembly occurs and whether complete fertilization is achieved in cat oocytes by interspecies sperm, we studied the fertilization processes of cat oocytes following the injection of cat, mouse, or hamster sperm. Male and female pronuclear formations were not different in the cat oocytes at 6 h following cat, mouse or hamster sperm injection. Microtubule asters were seen in all oocytes following intracytoplasmic injection of cat, mouse or hamster sperm. Immunocytochemical staining with a histone H3-m2K9 antibody revealed that mouse sperm chromatin is incorporated normally with cat egg chromatin, and that the cat eggs fertilized with mouse sperm enter metaphase and become normal 2-cell stage embryos. These results suggest that sperm aster formation is maternally dependent, and that fertilization processes and cleavage occur in a non-species specific manner in cat oocytes.

Direct numerical simulation of a turbulent boundary layer was performed to investigate the spatially coherent structures associated with very-large-scale motions (VLSMs). The Reynolds number was varied in the range Reθ = 570–2560. The main simulation was conducted by using a computational box greater than 50δo in the streamwise domain, where δo is the boundary layer thickness at the inlet, and inflow data was obtained from a separate inflow simulation based on Lund's method. Inspection of the three-dimensional instantaneous fields showed that groups of hairpin vortices are coherently arranged in the streamwise direction and that these groups create significantly elongated low- and high-momentum regions with large amounts of Reynolds shear stress. Adjacent packet-type structures combine to form the VLSMs; this formation process is attributed to continuous stretching of the hairpins coupled with lifting-up and backward curling of the vortices. The growth of the spanwise scale of the hairpin packets occurs continuously, so it increases rapidly to double that of the original width of the packets. We employed the modified feature extraction algorithm developed by Ganapathisubramani, Longmire & Marusic (J. Fluid Mech., vol. 478, 2003, p. 35) to identify the properties of the VLSMs of hairpin vortices. In the log layer, patches with the length greater than 3δ–4δ account for more than 40% of all the patches and these VLSMs contribute approximately 45% of the total Reynolds shear stress included in all the patches. The VLSMs have a statistical streamwise coherence of the order of ~6δ; the spatial organization and coherence decrease away from the wall, but the spanwise width increases monotonically with the wall-normal distance. Finally, the application of linear stochastic estimation demonstrated the presence of packet organization in the form of a train of packets in the log layer.

Direct numerical simulation (DNS) of a spatially developing turbulent boundary layer (TBL) over a wall roughened with regularly arrayed cubes was performed to investigate the effects of three-dimensional (3-D) surface elements on the properties of the TBL. The cubes were staggered in the downstream direction and periodically arranged in the streamwise and spanwise directions with pitches of px/k = 8 and pz/k = 2, where px and pz are the streamwise and spanwise spacings of the cubes and k is the roughness height. The Reynolds number based on the momentum thickness was varied in the range Reθ = 300−1300, and the roughness height was k = 1.5θin, where θin is the momentum thickness at the inlet, which corresponds to k/δ = 0.052–0.174 from the inlet to the outlet; δ is the boundary layer thickness. The characteristics of the TBL over the 3-D cube-roughened wall were compared with the results from a DNS of the TBL over a two-dimensional (2-D) rod-roughened wall. The introduction of cube roughness affected the turbulent Reynolds stresses not only in the roughness sublayer but also in the outer layer. The present instantaneous flow field and linear stochastic estimations of the conditional averaging showed that the streaky structures in the near-wall region and the low-momentum regions and hairpin packets in the outer layer are dominant features in the TBLs over the 2-D and 3-D rough walls and that these features are significantly affected by the surface roughness throughout the entire boundary layer. In the outer layer, however, it was shown that the large-scale structures over the 2-D and 3-D roughened walls have similar characteristics, which indicates that the dimensional difference between the surfaces with 2-D and 3-D roughness has a negligible effect on the turbulence statistics and coherent structures of the TBLs.