The cave-living and monobasic fungus gnat genus Speolepta Edwards is reviewed with a description of the first Nearctic species, Speolepta vockerothisp. nov., from Canada (caves in Ontario and British Columbia) and the United States (Alaska). Its morphology, life history, and biology are documented and compared with the single European species Speolepta leptogaster (Winnertz). A further new species, Speolepta orientalissp.nov., is described based on a single male from northern Vietnam, representing the first record of this genus from the Oriental Region.