Hubei province in China has had the most confirmed coronavirus disease 2019 (COVID-19) cases and has reported sustained transmission of the disease. Although Lu'an city is adjacent to Hubei province, its community transmission was blocked at the early stage, and the impact of the epidemic was limited. Therefore, we summarised the overall characteristics of the entire epidemic course in Lu'an to help cities with a few imported cases better contain the epidemic. A total of 69 confirmed COVID-19 cases and 11 asymptomatic carriers were identified in Lu'an during the epidemic from 12 January to 21 February 2020. Fifty-two (65.0%) cases were male, and the median age was 40 years. On admission, 56.5% of cases had a fever as the initial symptom, and pneumonia was present in 89.9% of cases. The mean serial interval and the mean duration of hospitalisation were 6.5 days (95% CI: 4.8–8.2) and 18.2 days (95% CI: 16.8–19.5), respectively. A total of 16 clusters involving 60 cases (17 first-generation cases and 43 secondary cases) were reported during the epidemic. We observed that only 18.9% (7/37) index cases resulted in community transmission during the epidemic in Lu'an, indicating that the scale of the epidemic was limited to a low level in Lu'an city. An asymptomatic carrier caused the largest cluster, involving 13 cases. Spread of COVID-19 by asymptomatic carriers represents an enormous challenge for countries responding to the pandemic.

The effect of holly polyphenols (HP) on intestinal inflammation and microbiota composition was evaluated in a piglet model of lipopolysaccharide (LPS)-induced intestinal injury. A total of twenty-four piglets were used in a 2 × 2 factorial design including diet type and LPS challenge. After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma. Our results indicated that HP enhanced intestinal disaccharidase activities and reduced plasma proinflammatory cytokines including TNF-α and IL-6 in LPS-challenged piglets. Moreover, HP up-regulated mRNA expression of intestinal tight junction proteins such as claudin-1 and occludin. In addition, bacterial 16S rRNA gene sequencing showed that HP altered hindgut microbiota composition by enriching Prevotella and enhancing SCFA production following LPS challenge. These results collectively suggest that HP is capable of alleviating LPS-triggered intestinal injury by improving intestinal disaccharidase activities, barrier function and SCFA production, while reducing intestinal inflammation.

In order to learn more about the feeding value of stovers and straws, seven diets were compared in one experiment and nine in another. The diets in the first experiment were: the upper and lower parts of lucerne (Medicago sativa) hay, the leaves, upper stem and lower stem of maize (Zea mays) stover and the leaves and stem of sorghum (Sorghum vulgare) stover. The diets in the second experiment were: the upper and lower parts of lucerne hay, the leaves, stem and whole stover of millet (Setaria italica), the straw and chaff of wheat (Triticum aestivum), rice (Oryza sativa) straw and sweet potato (Ipomoea batatas) haulm. The diets were fed to sheep to record rate of intake, voluntary intake and in vivo digestibility. The diets were analysed for cell wall (as neutral detergent fibre), crude protein and lignin. Organic matter degradation and cell wall degradation were recorded in nylon bags in the rumen of cattle.

The highest rates of intake (6·8–9·6 g dry matter/min) and voluntary intake (75–103 g dry matter/kg W0·75/day) were with sweet potato haulm and the upper, leafy part of lucerne hay. The voluntary intake of millet leaves, wheat chaff and the lower, stemmy part of lucerne hay was moderately high (46–70 g dry matter/kg W0·75/day). Voluntary intake of leaves was higher than that of stems in the case of millet, sorghum and to a lesser extent maize. The lowest rates of intake were with millet stem, sorghum stem and rice straw (1·8–2·4 g dry matter/min). In vivo digestibility of organic matter was highest (59–67%) for the upper part of lucerne hay, sweet potato haulm, the lower part of maize stem and millet leaves. The leaves of maize were less digestible than the stem, while the reverse was the case with millet. Cell wall content was particularly high (74–78% of dry matter) in millet stem, wheat straw and chaff, rice straw and sorghum stem. Lignin content was low (3·6–4·2% of dry matter) in millet leaves, maize leaves and the upper part of maize stem. Organic matter degradation after 24 h incubation in the rumen was highest (80%) in sweet potato haulm and lowest (39–44%) in millet stem, wheat straw and chaff and rice straw. Cell wall degradation after 96 h in the rumen was highest (76–81%) with the leaves of maize, sorghum and millet and lowest (40–59%) with lucerne hay, millet stem and stover, wheat straw and chaff and rice straw.