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Edited by
Daniel Benoliel, University of Haifa, Israel,Peter K. Yu, Texas A & M University School of Law,Francis Gurry, World Intellectual Property Organization,Keun Lee, Seoul National University
The Nagoya Protocol to the Convention on Biological Diversity (CBD) has threatened to impede access to genetic resources and related data for cross-border scientific research. In principle, every use of genetic resources would require a set of contracts under the CBD, in a “bilateral” regime. The related transaction costs could overwhelm many transnational research undertakings, affecting even public health responses to outbreaks and epidemics. However, the Nagoya Protocol also offers a unique opportunity to resolve this dilemma, despite struggles to define the meaning and coverage of “digital sequence information.” The coverage of genetic sequence data under the CBD remains controversial mainly because users do not know what the potential consequences of such coverage might ultimately entail. This chapter’s objective is to outline a type of coverage devised specifically for pathogens that would promote science, public health, and commercial applications while also protecting the interests of provider countries, supporting innovation, and addressing inequalities. The authors envision an agreed waiver for pathogen sequence data used for upstream scientific research purposes under the Nagoya Protocol, without compromising the duty of users to share benefits. This waiver should help alleviate the problems of definition and subject-matter coverage that have stymied multilateral action.
Airspace control plays an important role in the safety and fluidity of air traffic. A fundamental service for this purpose is audio communication through frequencies in the VHF bands. This paper describes the evaluation of the audio degradation of voice transmissions from control centre to the aircraft. The effects of more than one station broadcasting on the same frequency with carrier offset (climax mode) are analysed using perceptual evaluation of speech quality (PESQ) perceptual model. Comparative studies are performed to verify the degree of degradation of different audio transport systems and climax situation.
Climate trends and weather indicators are used in several research fields due to their importance in statistical modeling, frequently used as covariates. Usually, climate indicators are available as grid files with different spatial and time resolutions. The availability of a time series of climate indicators compatible with administrative boundaries is scattered in Brazil, not fully available for several years, and produced with diverse methodologies. In this paper, we propose time series of climate indicators for the Brazilian municipalities produced using zonal statistics derived from the ERA5-Land reanalysis indicators. As a result, we present datasets with zonal statistics of climate indicators with daily data, covering the period from 1950 to 2022.
Managing winter annual grass weeds has long been a challenge in the dryland regions of the Pacific Northwest (PNW) where soft white winter wheat is grown. The recent development of quizalofop-resistant (CoAXium) wheat varieties allows growers to use quizalofop (QP), a herbicide that inhibits acetyl-CoA carboxylase (ACCase) for postemergence grass control. Field experiments were conducted over two winter wheat growing seasons in 2021–2022 and 2022–2023 near Adams, OR, to evaluate QP efficacy on feral rye and for crop safety. Downy brome and jointed goatgrass control with QP were assessed in 2021–2022 and 2022–2023, respectively. QP treatments provided effective control of feral rye (≥95%), downy brome (≥87%), and jointed goatgrass (99%) regardless of rate, adjuvant, and spray volume tested. Spring-applied QP caused no injury to winter wheat. Results indicate that the QP-resistant wheat technology can help PNW wheat growers selectively control winter annual grasses.
This review summarizes what is currently known about herbicide resistance in Bromus spp. worldwide. Additional information on the biology and genetics of Bromus spp. is provided to further the understanding of resistance evolution and dispersal of the different species. Cases of herbicide resistance have been confirmed in Bromus catharticus Vahl., Bromus commutatus Schrad. (syn.: Bromus racemosus L.), Bromus diandrus Roth, Bromus japonicus Thunb. (syn.: Bromus arvensis L.), Bromus madritensis L., Bromus rigidus Roth (syn.: Bromus diandrus Roth ssp. diandrus), Bromus rubens L., Bromus secalinus L., Bromus sterilis L., and Bromus tectorum L. in 11 countries. Bromus spp. populations have evolved cross- and multiple resistance to six herbicide sites of action: acetyl-coenzyme A carboxylase, acetolactate synthase, photosystem II, very-long-chain fatty-acid, 5-enolpyruvylshikimate-3-phosphate synthase, and 4-hydroxyphenylpyruvate dioxygenase inhibitors. Resistance mechanisms varied from target-site to non–target site or a combination of both. Bromus spp. are generally highly self-pollinated, but outcrossing can occur at low levels in some species. Bromus spp. have different ploidy levels, ranging from diploid (2n = 2x = 14) to duodecaploid (2n = 12x = 84). Herbicide resistance in Bromus spp. is a global issue, and the spread of herbicide-resistance alleles primarily occurs via seed-mediated gene flow. However, the transfer of herbicide-resistance alleles via pollen-mediated gene flow is possible.
This work aimed to investigate the effects of early progeny exposure to methylglyoxal (MG), programming for metabolic dysfunction and diabetes-like complications later in life. At delivery (PN1), the animals were separated into two groups: control group (CO), treated with saline, and MG group, treated with MG (20 mg/kg of BW; i.p.) during the first 2 weeks of the lactation period. In vivo experiments and tissue collection were done at PN90. Early MG exposure decreased body weight, adipose tissue, liver and kidney weight at adulthood. On the other hand, MG group showed increased relative food intake, blood fructosamine, blood insulin and HOMA-IR, which is correlated with insulin resistance. Besides, MG-treated animals presented dyslipidaemia, increased oxidative stress and inflammation. Likewise, MG group showed steatosis and perivascular fibrosis in the liver, pancreatic islet hypertrophy, increased glomerular area and pericapsular fibrosis, but reduced capsular space. This study shows that early postnatal exposure to MG induces oxidative stress, inflammation and fibrosis markers in pancreas, liver and kidney, which are related to metabolic dysfunction features. Thus, nutritional disruptors during lactation period may be an important risk factor for metabolic alterations at adulthood.
Amid widespread occurrence of herbicide-resistant weeds in the United States, the use of PRE herbicides and cover crops have resurged once again as important strategies for weed management in cropping systems. The objective of this experiment was to evaluate the length of soil residual weed control from PRE soybean herbicides and the detrimental impact of these herbicides on cover crop species using field treated soil in greenhouse bioassays. Greenhouse bioassays were conducted using soil from field experiments conducted in 2018 and 2019 in Arlington and Lancaster, WI. PRE herbicides consisted of imazethapyr, chlorimuron-ethyl, and cloransulam-methyl (acetolactate synthase [ALS] inhibitors); metribuzin (photosystem II [PS II] inhibitor); sulfentrazone, flumioxazin, and saflufenacil (protoporphyrinogen oxidase [PPO] inhibitors); acetochlor, S-metolachlor, dimethenamid-P, and pyroxasulfone (very long-chain fatty acid [VLCFA] inhibitors); and a nontreated control. Greenhouse bioassays were conducted using soil (depth, 0 to 10 cm) sampled at 0, 10, 20, 30, 40, and 50 d after treatment (DAT). Palmer amaranth and giant foxtail (weeds), and radish and cereal rye (cover crops) were used as bioindicators of herbicide levels in the soil. Bioassay results showed extended soil residual control of Palmer amaranth with sulfentrazone and pyroxasulfone; extended residual control of giant foxtail was observed with pyroxasulfone and S-metolachlor. Chlorimuron-ethyl and metribuzin were the most injurious herbicides to radish and cereal rye shortly after application, respectively, but minimal injury was observed from soil samples collected 50 DAT, indicating the use of PRE and fall-seeded cover crops in southern Wisconsin can be compatible. These results can support growers and practitioners with selection of effective PRE herbicides for Palmer amaranth and giant foxtail control and reduced impact on fall-seeded radish and cereal rye cover crops, altogether leading to more effective, diverse, and sustainable weed management programs.
The production of specialty coffee has several factors and parameters that are added up in the course of production, so that the quality is expressed in the act of consumption. Based on this scenario, this study included the analysis of ten genotypes of arabica coffee, the materials being subjected to irrigated and rainfed water regimes, in a low altitude region, to identify responses for sensory and physical–chemical quality. The genotypes were evaluated in a split-plot scheme with a randomized block design, with three replications. Arabica coffee fruits were harvested with 80% cherry seeds and processed by the wet method. Subsequently, the characteristics related to physical–chemical and sensory analyses were evaluated. The genotypes of the Paraíso group showed great variability for the physical–chemical and sensory variables for rainfed and irrigated regimes. The genotypes of the Catuaí group, however, showed less variability for sensory characteristics in both cultivation environments and for physical–chemical characteristics in the irrigated regime. In the sensorial data set, the genotypes Catuaí 144 CCF and Catuaí 144 SFC (when irrigated) and Paraíso H 419-3-3-7-16-2, Paraíso H 419-3-3-7-16-11 and Catucaí 24-137 (rainfed cultivation), are more favourable to the production of specialty coffee at low altitude.
Although several electrophysiological studies have demonstrated the role of theta band during the execution of different visuospatial attention tasks, this study is the first to directly investigate the role of theta power during the planning, execution and cognitive control of SEM.
Objective
Saccadic eye movements (SEMs) represent the first stage of visual sensorimotor integration and are relevant for the visuospatial attention. The current study aims to address this issue by investigating absolute theta power over the frontal cortex during the execution of stimulus- and memory-driven SEMs.
Methods
12 healthy volunteers (3 male; mean age: 26.25), performed two tasks involving different conditions in the planning, execution and cognitive control of SEMs while their brain activity pattern is recorded using quantitative electroencephalography.
Results
We found an interaction between SEM condition (memory- vs stimulus-driven) and electrode (F3, F4, Fz), and a main effect of time point and electrode. Our key finding revealed that the stimulus presentation induces different patterns over frontal theta power increase between the left and right hemisphere.
Conclusions
We conclude that right and left frontal regions are an important factor to discriminate between memory- versus stimulus-driven SEMs, and speculate on their role for different levels within the visuospatial attention.
It is known that the level of dietary protein modulates the enzymatic activity of the digestive tract of fish; however, its effect at the molecular level on these enzymes and the hormones regulating appetite has not been well characterised. The objective of this study was to evaluate the effect of CP on the activity of proteases and the expression of genes related to the ingestion and protein digestion of juveniles of red tilapia (Oreochromis sp.), as well as the effects on performance, protein retention and body composition of tilapia. A total of 240 juveniles (29.32 ± 5.19 g) were used, distributed across 20 tanks of 100 l in a closed recirculation system. The fish were fed to apparent satiety for 42 days using four isoenergetic diets with different CP levels (24%, 30%, 36% and 42%). The results indicate that fish fed the 30% CP diet exhibited a higher growth performance compared to those on the 42% CP diet (P < 0.05). Feed intake in fish fed 24% and 30% CP diets was significantly higher than that in fish fed 36% and 42% CP diets (P < 0.05). A significant elevation of protein retention was observed in fish fed with 24% and 30% CP diets. Fish fed with 24% CP exhibited a significant increase in lipid deposition in the whole body. The diet with 42% CP was associated with the highest expression of pepsinogen and the lowest activity of acid protease (P < 0.05). The expression of hepatopancreatic trypsinogen increased as CP levels in the diet increased (P < 0.05) up to 36%, whereas trypsin activity showed a significant reduction with 42% CP (P < 0.05). The diet with 42% CP was associated with the lowest intestinal chymotrypsinogen expression and the lowest chymotrypsin activity (P < 0.05). α-amylase expression decreased with increasing (P < 0.05) CP levels up to 36%. No significant differences were observed in the expression of procarboxypeptidase, lipase or leptin among all the groups (P > 0.05). In addition, the diet with 42% CP resulted in a decrease (P < 0.05) in the expression of ghrelin and insulin and an increase (P < 0.05) in the expression of cholecystokinin and peptide yy. It is concluded that variation in dietary protein promoted changes in the metabolism of the red tilapia, which was reflected in proteolytic activity and expression of digestion and appetite-regulating genes.
Most mental disorders begin during adolescence and early adulthood (18–24-years-old), highlighting the importance of understanding the onset and progression of mental disorders among youths. Although progress has been made, namely by creating youth mental health transition services, gaps continue to exist. Locally, our recently constituted young adults unit aimed to minimize discontinuities in care.
Objectives/aims
To characterize a population of young adult psychiatric outpatients, regarding socio-demographic and clinic variables.
Methods
Socio-demographic and clinic characterization of young adult psychiatric outpatients observed during 1st January 2015–30th July 2016.
Results
Two hundred and fifty-five outpatients were observed: 64.3% females and 35.7% males, average age 20.56-years-old (median 20). Most lived in urban areas (59.4%), with their parents (27.8%), were students (80.4%), attending secondary school (36.1%). A total of, 27.5% were referenced by an emergency department, and adjustment disorders (ICD-10 F43.2) were the most frequent diagnosis (21.6%). Regarding suicidal behaviours and self-harm, 9.8% did self-cutting. A vast majority did not have previous psychiatric hospitalizations–only 5.9% outpatients had at least one. In total, 39.8% were medicated with antidepressants (1/3 of which in association with other drugs), and about 38.8% received cognitive-behavioural interventions. About 2/3 of patients (66.3%) remained in care and only about 1/6 (15.7%) were discharged.
Conclusions
Our typical youth psychiatric outpatient was of female gender, student, living with its parents. Adjustment disorders were the most frequent diagnosis, and antidepressants were the main psychopharmacologic option, often combined with other psychotropic drugs. In the future, psychotherapy interventions should be more widely available, namely group psychotherapy.
Disclosure of interest
The authors have not supplied their declaration of competing interest.
The refugee population has been a growing concern to the developed countries in general and to Europe in particular. The recent mass migrations are changing the population that is getting to the psychiatric hospitals, not only culturally but also pathologically. The aim of this study is to characterize the refugee population that contacts with the clinic of transcultural psychiatry in Centro Hospitalar Psiquiátrico de Lisboa, understanding the most frequent pathologies and nationalities. The methods used consisted in analyzing the refugee population that attended a psychotherapeutic group and consultation in the Transcultural clinic of Centro Hospitalar Psiquiátrico de Lisboa during the past year. Analyzing the population, 66 refugees were in contact with the transcultural clinic, 44 of which were men, being the other 22 women, representing a total of 23 countries. The more frequent nationality was Iranian (20) and the most frequent diagnosis was “adjustment disorder and anxiety” (38). We can reach the conclusion that more refugee men contact with our psychiatric hospital than women, accounting for 66, 6% of the total; 30, 3% of the refugees were from Iran, followed by Pakistan with 10, 6%. The fact that the most frequent diagnosis is adjustment disorder and anxiety, accounting for 57, 6% of the sample, seems to point out the extreme stress refugees undergo.
Disclosure of interest
The authors have not supplied their declaration of competing interest.
Psychopathology rating scales have been widely used. Studies evaluating the congruence between self and observer-reported ratings show inconsistent results.
Objectives or aims
Determine whether there was consistency between psychopathology as assessed by patients and clinicians’ assessment in outpatients observed in the Young Adult Unit of our Psychiatry Department (Coimbra Hospital and University Centre-Portugal).
Methods
Socio-demographic characterization was undertaken with young adult outpatients observed during nineteen months (1st January 2015–31st July 2016). Brief Symptom Inventory–53 items (BSI-53) and Brief Psychiatric Rating Scale (BPRS) were applied at the first clinical evaluation. Spearman correlation coefficient between General Severity Index (GSI) of BSI-53 and BPRS total score was calculated.
Results
During the mentioned interval 255 outpatients were observed: 64.3% females and 35.7% males; aged between 17 and 39 years old (average: 20.56; median: 20). Fifty percent were diagnosed with neurotic, stress-related and somatoform disorders (ICD-10 F40-48) and 14.1% with mood disorders (ICD-10 F30-39). BPRS and BSI-53 rating scales were administered to 55 patients: 72.5% females and 27.5% males; average age 21.2. A statistical significant correlation was found between BPRS total and GSI score.
Conclusions
Several factors can determine the accuracy of psychopathology self-assessment, including diagnosis and severity of illness. In fact, studies show strong correlation between self and observer assessment of depressive and anxious psychopathology, but no correlation in psychotic psychopathological dimensions. Therefore, the correlation found in this population can be explained by the fact that the majority of patients were diagnosed with neurotic, stress-related and somatoform and mood disorders.
Disclosure of interest
The authors have not supplied their declaration of competing interest.
Approximately a third of patients with schizophrenia show limited response to antipsychotic medication. As several studies have been suggesting new classifications to schizophrenia, our aim is to review different hypothesis and seek a new way of approaching patient's treatment in day-to-day practice.
Methods
The methods we used consisted on reviewing several papers that have recently been published on the area of classification and treatment of schizophrenia, considering an approach to the findings that enables a practical and clinical advantage in the area.
Discussion
New studies suggest that neuroimaging measures of dopamine and glutamate function might provide a means of stratifying patients with psychosis according to their response to treatment. Some of those studies associate treatment response with the anterior cingulate level of glutamate and striatal dopamine synthesis capacity. Other study identified three biotypes with different outcomes to psychosis, reaching a stronger association between biotypes as predictors of illness severity than the DSM-V classification. If a correlation between these studies was found, we would be able, in theory, to predict the response to treatment using simple and affordable neurobiological measures.
Conclusion
Associating the anterior cingulate glutamate levels, the striatal dopamine synthesis capacity and biotypes hypothesis in schizophrenia, one can expect to be possible to predict the degree of response to treatment, based on more affordable methods to day-to-day clinicians than the measure of neurotransmitter levels, enabling the regular clinicians to narrow their pharmacological options for patients, achieving better results in the approach to schizophrenia.
Disclosure of interest
The authors have not supplied their declaration of competing interest.
Research has shown that PTSD is prevalent among firefighters and police forces and that Quality of Life (QoL) is seriously compromised in individuals suffering from PTSD. However, QoL studies with these professionals are scarce. This study results from a screening program held by the Portuguese Red Cross (PRC) aiming to analyze predictors of QoL. Participants were 95 firefighters and municipal police officers. They answered the Posttraumatic Stress Disorder Checklist (PCL-5) in order to evaluate the prevalence of PTSD symptoms, as well as measures of social support (3-Item Oslo Social Support Scale) and QoL (EUROHIS-QOL-8). From the results, there were no group differences regarding total PTSD, social support or QoL and 10% of participants reported enough symptoms to PTSD diagnostic. Social Support and PTSD explained 25% of QoL variance, PTSD symptoms explaining 10% (negative beta) and, in the second step, social support explained 15%. The results suggest that it would be important to include QoL as an outcome measure in clinical and research work in these populations, with special attention to PTSD and social support.
Disclosure of interest
The authors have not supplied their declaration of competing interest.
Portuguese young adults (15–34 years old) were more frequent engaged in substance abuse (1.2%) than the general population, according to the Portuguese substance use annual report (2014). Alcohol was the most frequent substance use on youth adults. Cannabis was the most frequent illicit substance used (23.9% of users were considered dependent), with higher prevalence than previously reported. LSD (0.4%) use was also higher among young adults than in previous studies. Cocaine (0.4%), heroine/opiates (0.4%), ecstasy (0.3%), and hallucinogenic mushrooms (1.1%) had their consumption lowered among young adults.
Objectives/aims
To characterize a population of young adult psychiatric outpatients, regarding substance use and associated risk.
Methods
Socio-demographic characterization of our young adult unit outpatient users between 1st January 2015–31st July 2016. Substance use was assessed with the Portuguese version of Alcohol, Smoking and Substance Involvement Screening Test (ASSIST).
Results
A total of 255 outpatients were observed during the timeframe; 58 outpatients were assessed with ASSIST: 44 females (75.9%) and 14 males (24.1%), aged between 16–33 years old (average: 20.95; median: 19.50). For tobacco, 3.5% had high risk, 37.9% moderate risk and 58.6% low risk; 22.4% had moderate risk of alcohol, 13.8% moderate risk of cannabis, and 20.7% moderate risk of tranquilizers. For others substances (cocaine, stimulants, inhalants, hallucinogens, and opiates) the risk was low.
Conclusions
In our sample, alcohol and cannabis use had the highest risk, as reported in the National Annual Report; however, we observed a moderate risk for tranquilizers use. In future care planning, youth mental health should address tobacco and alcohol abstinence, and preventive measures regarding anxiolytics should be undertaken, such as banning sale without medical prescription.
Disclosure of interest
The authors have not supplied their declaration of competing interest.
Modern psychiatry includes within its purposes to reintegrate patients into society. Our work's goal is to evaluate in terms of outcome, the current status of patients that underwent the rehabilitation program in a clinic in Centro Hospitalar Psiquiátrio de lisboa, in Lisbon.
Methods
The methods consisted of retrospective evaluation of the sample of patients that successfully completed the rehabilitation program between 2012 and 2015. Gender, age, provenience, previous and current socio-economic situations were taken into account.
Discussion
Twelve patients concluded the program in the timeframe studied: 64 % males and 36 % between the ages of 36 and 40 and more than half were in homelessness situations. They progressed in increasing levels of autonomy and by the program's end, ten were dismissed and two were still waiting for housing. Ten of the patients are now living with their families or have been integrated in social housing program. Two await conditions to leave the hospital.
Conclusion
The program had a positive impact on the life of all patients that have engaged in it. Only two remain waiting social housing responses. Although the results are satisfactory, the number of patients admitted must improve in coming years.
Disclosure of interest
The authors have not supplied their declaration of competing interest.
Alcohol use disorders (AUD) is a preventable cause of significant morbidity and mortality worldwide. AUD is a heterogeneous disorder stemming from a complex interaction of neurobiological, genetic, and environmental factors. To achieve treatment effectiveness this heterogenity should be considered, as well as safety.
Objectives
Review mechanisms underlying alcohol addiction in order to work out new, more effective treatment strategies.
Aim
To update on treatment for alcoholism.
Methods
A literature search was performed on PubMed database.
Results
Alcohol dependence is a chronic, relapsing condition in which there is evidence of significant change in the motivation and control systems in the brain. Increasingly drug therapy is focused not just on the treatment of the acute withdrawal syndrome, but on modifying these other dysregulated brain systems. Of the numerous neurotransmitter systems that have been identified for the development of new medicines, the most promising compounds appear to be those that modulate the function of opioids, glutamate with or without gamma-aminobutyric acid, and serotonin. Other putative therapeutic medications including direct modulators of dopamine function and enzyme inhibitors also shall be discussed. At present, only four medications are approved for the treatment of alcohol dependence in Europe, that is naltrexone, acamprosate, disulfiram and the most recent nalmefene. Among other promising strategies the following drugs are mentioned: baclofen, topiramate, ondansetron, aripiprazole, rimonabant and varenicline.
Conclusions
Pharmacological development remains a high priority in the alcoholism field. Drugs have different safety profiles that need to be balanced with the treatment objective, individual patient preferences and comorbid conditions.
Disclosure of interest
The authors have not supplied their declaration of competing interest.