The stable mechanical property polystyrene (PS) nanotubes with array structure were first prepared by filtrating a solution or melt of normal molecular weight PS (Mn ≈ 180,000) into anodic aluminum oxide (AAO) template with only 200-nm pores. The results of transmission electron microscopy and scanning electron microscopy illuminated that PS tubules were successfully obtained and the wall thickness of PS tubules prepared by the 2.5 wt%, 5.0 wt%, and 10.0 wt% PS solution were respectively 50 nm, 70 nm, and 80 nm. The structure of polymer nanotubes depends strongly on the concentration of PS solution. It was found that the wall of the nanotubes derived from melt is thicker than that of the nanotubes from solution. When the polymer solution wets AAO template, a thin polymer film will be formed on the inner porous wall. The thin film/AAO composite membrane acts as a “second-order” template and is soaked repeatedly. So PS solution wets the template and forms nanotubes according to the multi-time wetting mechanism.