WeedSOFT is a state-of-the-art decision support system for weed management in the north central region of the United States, but its accuracy to predict corn yield loss associated with later-emerging weed communities has not been adequately assessed. We conducted experiments in 2004 and 2005 to compare observed and predicted corn yield related to four establishment times of mixed-species weed communities for validation of competitive index modifier (CIM) values in WeedSOFT. Weed communities were established at VE, V2, V4, and V6 corn (emergence, second-leaf, fourth-leaf, and sixth-leaf stages, respectively), and consisted largely of annual grass and moderately competitive annual broadleaf species. Compared to weed-free corn, yield loss occurred in each of seven site-years for weed communities established at VE corn, but in only one site-year for communities established at V2 corn. No corn yield loss was associated with weed communities established at V4 or V6 corn. For communities established at VE corn, predicted corn yield differed from observed yield in all but one site year, with predicted yield less than observed yield in three site-years, and greater than observed yield in two site-years; however, nonlinear regression analyses of yield data pooled over site-years showed that fitted values were similar between predicted and observed yield. For communities established at V2 and V4 corn, predicted yield was less than observed yield in six and five site-years, respectively. For communities established at V6 corn, predicted yield was less than observed yield in three of six site-years, but was similar to observed yield in three of six site-years. These results indicated that the CIM values in WeedSOFT tended to overestimate the competitiveness of late-emerging weed communities. Corn yield data from a pooled analysis of all site-years were used to generate a revised set of growth stage CIM values, which improved the accuracy of predicted corn yield. These results should improve weed management decisions and reduce the need for herbicide applications to late-emerging weeds.