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Tuber borchii mycelium (strain 1BO) is able to utilise glucose, fructose or mannitol in the culture medium as a carbohydrate source. Since sugars not only function as a metabolic resource and structural constituent of cells, but also act as important regulators of various processes, we investigated if high sugar concentrations could influence fungal growth and development. The studies performed in this paper revealed that fructose or mannitol used at high concentration (50 g l−1) in the culture medium do not influence the growth and the biochemical responses of fungus but the growth of T. borchii mycelium is subject to glucose repression. In experiments with a high glucose concentration (50 g l−1) and with 2-deoxyglucose, a non-metabolisable glucose analogue, the growth of T. borchii was halved with respect to the control (10 g l−1 of glucose). The morphological and biochemical analyses revealed that the hyphae were metabolically and functionally active, but the activity of mannitol dehydrogenase was reduced to one-third in the high glucose treatment. This is the first evidence of glucose repression of growth and activity in the ascomycetous ectomycorrhizal fungus T. borchii.
This paper reports a study aimed at gaining new information on the molecular composition of fruitbodies of Tuber spp. The presence
of proteins homologous to TBF-1, which is highly specific for the fruitbody-phase of Tuber borchii, has been investigated in other
white truffles. SDS-PAGE analyses revealed that only Tuber dryophilum fruitbodies possess a similar protein. This protein was purified
by HPLC and partially sequenced, confirming a high degree of homology with TBF-1. Several PCR analyses of the genomic DNA,
were performed to evaluate whether the absence of proteins homologous to TBF-1 in other white truffle species was a result of the
absence of the coding genes. T. dryophilum gave an amplification product corresponding to the entire gene (tdf-1), but no products
were obtained from the other species. Tdf-1 was sequenced and its organisation studied since it is one of the first genes isolated
from a Tuber species. The deduced amino acid sequence was compared to that of TBF-1, to evaluate the presence of conserved
regions, in an attempt to gain new information about their role in fruitbody formation.
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