Storytelling is increasingly recognized as a culturally relevant, human-centered strategy and has been linked to improvements in health knowledge, behavior, and outcomes. The Community Engagement Program of the Johns Hopkins Institute for Clinical and Translational Research designed and implemented a storytelling training program as a potentially versatile approach to promote stakeholder engagement. Data collected from multiple sources, including participant ratings, responses to open-ended questions, and field notes, consistently pointed to high-level satisfaction and acceptability of the program. As a next step, the storytelling training process and its impact need to be further investigated.

Background: The capacity to monitor the emergence of carbapenemase-producing organisms (CPO) is critical in limiting transmission. CPO-colonized patients can be identified by screening rectal specimens for carbapenemase genes and the Cepheid GeneXpert Carba-R (XCR), the only FDA-approved test, is limited to 5 carbapenemase genes and cannot identify the bacterial species. Objective: We describe the development and validation of culture-based methods for the detection of CPO in rectal cultures (RCs) and nonrectal cultures (NRCs) of tracheal aspirate and axilla-groin swabs. Methods: Colonization screening was performed at 3 US healthcare facilities; specimens of RC swabs and NRC ESwabs were collected. Each specimen was inoculated to a MacConkey broth enrichment tube for overnight incubation then were subcultured to MacConkey agar with meropenem and ertapenem 10 µg disks (BEMA) and CHROMagar KPC (KCHR) or CHROMagar Acinetobacter (ACHR). All media were evaluated for the presence of carbapenem-resistant organisms; suspect colonies were screened by real-time PCR for the most common carbapenemase genes. MALDI-TOF was performed for species identification. BEMA, a previously validated method, was the comparator for 52 RCs; clinical culture (CC) served as the comparator method for 66 NRCs. Select CPO-positive and -negative specimens underwent reproducibility testing. Results: Among 56 patients undergoing colonization screening, 12 (21%) carried a CPO. Only 1 patient had CPO solely from RC. Also, 6 patients had both CPO-positive RC and NRC, and 5 patients only had a CPO-positive NRC. Of the latter, 4 had a CPO-positive tracheal specimen, and 1 had a positive culture from both tracheal and axilla-groin specimens. Sensitivity of BEMA (70%) for NRC was lower than for KCHR (96%) and ACHR (88 %) for all specimens. All methods showed a specificity of 100% and reproducibility of 92%. The detected CPO included OXA-23–positive Acinetobacter baumannii, NDM-positive Escherichia coli, KPC-positive Pseudomonas aeruginosa and 4 genera of KPC-positive Enterobacteriaceae. Conclusions:The addition of nonrectal specimens and use of selective media contributed to increased sensitivity and enhanced identification of CPO-colonized patients. Positive cultures were equally distributed among the 3 specimen types. The addition of the nonrectal specimens resulted in the identification of more colonized patients. The culture-based method was successful in detecting an array of different CPOs and target genes, including genes not detected by the Carba-R assay (eg, blaOXA-23-like). Enhanced isolation and characterization of CPOs will be key in aiding epidemiologic investigations and strengthening targeted guidance for containment strategies.

Funding: None

Disclosures: We discuss the drug combination aztreonam-avibactam and acknowledge that this drug combination is not currently FDA approved.

The Richtmyer–Meshkov instability (RMI) is experimentally investigated in a vertical shock tube using a broadband initial condition imposed on an interface between a helium–acetone mixture and argon (Atwood number $A\approx 0.7$). In the present work, a shear layer is introduced at the interface to serve as a statistically repeatable, broadband initial condition to the RMI, and the density interface is accelerated by either an $M=1.6$ or $M=2.2$ planar shock wave. The development of the ensuing mixing layer is investigated using simultaneous planar laser-induced fluorescence (PLIF) and particle image velocimetry (PIV). PLIF images are processed to reveal the light-gas mole fraction, while PIV particle image pairs yield corresponding two-component planar velocity results. Field structure and distribution are explored through probability density functions (PDFs), and a decomposition is performed on concentration and velocity results to obtain a mean flow field and define fluctuations. Simultaneous concentration and velocity field measurements allow – for the first time in this regime – experimentally determined turbulence quantities such as Reynolds stresses, turbulent mass-flux velocities and turbulent kinetic energy to be obtained. We show that by the latest times the mixing layer has passed the turbulent threshold, and there is evidence of turbulent mixing occurring sooner for the higher Mach number case. Interface measurements show nonlinear growth with a power-law fit to the thickness data, and that integral measurements of mixing layer thickness are proportional to threshold measurements. Spectral analysis demonstrates the emergence of an inertial range with a slope ${\sim}k^{-5/3}$ when considering both density and velocity effects in planar turbulent kinetic energy (TKE) measurements.

Weeds generally occur in patches in production fields. Are these patches spatially and temporally stable? Do management recommendations change on the basis of these data? The population density and location of annual grass weeds and common ragweed were examined in a 65-ha corn/soybean production field from 1995 to 2004. Yearly treatment recommendations were developed from field means, medians, and kriging grid cell densities, using the hyperbolic yield loss (YL) equation and published incremental YL values (I), maximum YL values (A), and YL limits of 5, 10, or 15%. Mean plant densities ranged from 12 to 131 annual grasses m−2 and < 1 to 37 common ragweed m−2. Median weed densities ranged from 0 to 40 annual grasses m−2 and were 0 for common ragweed. The grass I values used to estimate corn YL were 0.1 and 2% and treatment was recommended in only 1 yr when the high I value and either the mean or median density was used. The grass I values used for soybean were 0.7 and 10% and estimated YL was over 10% all years, regardless of I value. The common ragweed I values were 4.5 and 6% for corn and 5.1 and 15.6% for soybean. On the basis of mean densities, fieldwide treatment would have been recommended in 6 of 9 yr but in no years when the median density was used. Recommendations on the basis of grid cell weed density and kriging ranged from > 80% of the field treated for grass weeds in 3 of 4 yr in soybean to < 20% of the field treated for common ragweed in 2002 and 2004 (corn). Grass patches were more stable in time, space, and density than common ragweed patches. Population densities and spatial distribution generally were variable enough so that site-specific information within this field would improve weed management decisions.

Khirbet Karhasan was excavated by the British Archaeological Expedition to Iraq, as part of the Saddam Dam Salvage Project. The excavations brought to light a hoard of Middle Assyrian faience ornaments, which formed a coherent assemblage. Collections of similar ornaments are known from Assur and Nimrud where they have been variously interpreted as decorating the rich garments for a god, a divine image, and even the walls of temples. In the absence of any other persuasive view a hypothesis is advanced based on the evidence of archaeological context, iconographic associations and a viable reconstruction, that the ornaments are equally adaptable to the decoration of ceremonial horse-harness.

The tell of Khirbet Karhasan is situated on the right bank of the Tigris approximately 75 km north-west of Mosul. The site on the edge of a prominent terrace overlooks a broad floodplain. In 1986, prior to the flooding of the site, the Tigris channel was 3 km north of the tell. The tell was sheltered by low hills which formed the western slope of the valley some 500 m to the south of the site. The hills rose some 40 m above the tell to approximately 50 m above the floodplain.

The 1963 excavation at Palaikastro-Kastri in eastern Crete produced about 150 marine shells. The species preserved are listed in Table 1, and their date and context in Table 2. Significant shells from the collection are compared with other Minoan examples in Part I. The shell evidence for purple-dye production at Palaikastro is studied along with the other evidence from the Bronze Age Mediterranean in Part II.

Shells from the excavation of the West Court House at Knossos are analysed and compared with those from other Neolithic and Early Minoan sites.

In questo secondo rapporto sugli scavi della Schola Praeconum in Palatino, gli AA. presentano materiali da due contesti tardo-antichi: SP I, un riempimento datato al periodo 430–440 c. e SP II, un' altero riempimento probabilmente databile ai primi decenni del VII secoto. Dal contesto SP I si presenta i vetri, i bolli laterizi, i semi e gli insetti; dal SP II, le monete, le ceramiche e le lucerne. Il materiale ceramico dal contesto SP II stimola alcune ipotesi sui rapporti commerciali di Roma nel VII secolo. Infine, due appendici presentano una discussione delle iscrizioni dipinte sulle anfore di argilla 14 e un' analisi del contenuto delle anfore importate da Bi e Gaza in Palestina.

Finds of the Mediterranean Cone Shell (Conus mediterraneus Hwass in Bruguière 1792 = ventricosus Gmelin) from Neolithic and Bronze Age Greece are listed, and their use discussed.

La cosiddetta Schola Praeconum si trova nella parte nord-ovest del Palatino sovrastante il Circo Massimo. Un sondaggio, effettuato nel 1978, rivelò un massiccio riempimento databile, sulla base delle 38 monete identificate, al secondo quarto del V secolo d.C., forse tra il 430 e il 440 circa. Il riempimento conteneva un'enorme quantità di ceramiche—terra sigillata chiara, lucerne (per la maggior parte di produzione locale), anfore commerciali, ecc. L'abbondante campione di fauna mostra soprattutto la presenza di bovini, pecore e maiali. Uno studio dei dati inerenti alla macellazione prova che il bestiame fosse sistematicamente allevato per il mercato della carne. La presenza delle anfore commerciali e del bestiame allevato per la tavola ci fa pensare alle informazioni sull'annona contenute nel Codice Teodosiano.

Tomb XVIII in the Gypsades cemetery, south of the Knossos Palace, was excavated by Sinclair Hood, George Huxley, and Nancy Sandars in 1955 and published in 1959.1

The main chamber contained at least 9 burials. Only a handful of objects were found associated with these burials: a small bronze bead with the skull of Skeleton II, 4 small, plain vessels with the skull of Skeleton III, silver ear-rings with Skeleton IV, a lentoid sealstone of hammered silver or meteorite with Burial VII, and a small bronze link with Burial VIII.

Since time immemorial attractive colours have held man's attention. Occasionally he has assigned to them symbolic significance, often as religious or status insignia. The various shades of reds and purples have been especially chosen; some see in them the colour of blood, the sun or fire. The presence of ochre and haematite in Palaeolithic burials may be the first instance of the high regard for these colours.

Probably the most famous red–purple colour associated with religion, status and general elitism is the shell-based purple-dye of the ancient Mediterranean, known variously as Royal purple, Tyrian purple or Imperial purple. The recent discovery of crushed purple-dye producing shells at Berenice provided a welcome addition to this field, a study which Alexander Dedekind, in the 1890s, termed “Pourprologie”.

Before examining the Berenice shells and their context a brief survey will be made of the world-wide usage of shell purple-dye, its Mediterranean origin and distribution, the basic method of production and the contemporary Mediterranean history of the Berenice finds.

Purple-dye is obtained from the hypobranchial gland found in the mantle cavity of living Murex and Thais (or related Purpura and Nucella) marine snails. The purpose of this gland is unclear, though it may be the reason such shells are avoided as food by marine life (and are thus not a useful fish bait). The dye might also be ejected by the mollusc as a defence mechanism, much like the black ink of the octopus or squid.