Age misreporting is a common phenomenon in Demographic and Health Surveys, and there are numerous reasons for this. The trend and pattern of disparity in age heaping vary between countries. The present study assesses age heaping in the selected South Asian countries of Afghanistan, India, Nepal, Bangladesh and Pakistan using data from the most recent round of the Demographic and Health Survey. The respondent sample sizes were 203,703 for Afghanistan, 2,869,043 for India, 49,064 for Nepal, 81,618 for Bangladesh and 100,868 for Pakistan. Age heaping was assessed by respondent’s age, education level, sex and level of education. Whipple’s index was calculated to assess systematic heaping on certain ages as a result of digit preference. Bangladesh, Afghanistan and India showed stronger preference for ages ending with the digits ‘0’ and ‘5’ compared with Pakistan and Nepal among uneducated respondents. On the other hand, strong avoidance of ages ending in the digits ‘1’, ‘4’ and ‘9’ was observed in Bangladesh, Afghanistan and India. However, urban–rural place of residence was not found to be associated with digit preference in the study countries. Among males, age misreporting with the final digits ‘0’ and ‘5’ was highest in Bangladesh, followed by Afghanistan and India, and Nepal showed the least displacement. Strong digit preference and avoidance, and upper age displacement, were witnessed in the surveys conducted in Bangladesh, Afghanistan and India on the parameters of sex and education level. Innovative methods of data collection with the measurement and minimization of errors using statistical techniques should be used to ensure accuracy of age data.

The study aims to estimate and compare the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) seroprevalence, the fraction of asymptomatic or subclinical infections in the population, determine the demographic risk factors and analyse the antibody development at different time points among adults in Bhubaneswar city, India. This was a serial three-round cross-sectional, community-based study where participants were selected from the residents of Bhubaneswar city using multi-stage random sampling. Blood samples were collected during household visits along with demographic and clinical data from every participant. Total anti-SARS-CoV-2 antibody present in serum was assessed using the electro-chemiluminescence immunoassay platform. Temporal comparisons of the community seroprevalence were performed against the detected number of cumulative cases, active cases, recoveries and deaths. A total of 3693 participants were enrolled in this study with a cumulative non-response rate of 18.33% in all the three rounds. The gender-weighted seroprevalence for the city in the first round was 1.55% (95% confidence interval (CI) 0.84–2.58), second round was 5.27% (95% CI 4.13–6.59) and in the third round was 49.04% (95% CI 46.39–51.68). In the first round, the seroprevalence was found to be highest in the elderly population, whereas the seroprevalence for the second and third phases was highest in the age group of 30–39 years. Seroprevalence showed an increasing trend over the three time periods, with the highest seropositivity rates among individuals sampled between 16 and 18 September 2020. By the third round, 93.93% of those who had previously been tested positive by real-time reverse transcription polymerase chain reaction had seroconversion and 46.57% of those who had been tested negative also showed seroconversion. Infection to case ratio during first round was 27.05, for second round and third round it was 5.62 and 17.91, respectively.

Background: Healthcare-associated infections (HAIs) are a major global threat to patient safety. Systematic surveillance is crucial for understanding HAI rates and antimicrobial resistance trends and to guide infection prevention and control (IPC) activities based on local epidemiology. In India, no standardized national HAI surveillance system was in place before 2017. Methods: Public and private hospitals from across 21 states in India were recruited to participate in an HAI surveillance network. Baseline assessments followed by trainings ensured that basic microbiology and IPC implementation capacity existed at all sites. Standardized surveillance protocols for central-line–associated bloodstream infections (CLABSIs) and catheter-associated urinary tract infections (CAUTIs) were modified from the NHSN for the Indian context. IPC nurses were trained to implement surveillance protocols. Data were reported through a locally developed web portal. Standardized external data quality checks were performed to assure data quality. Results: Between May 2017 and April 2019, 109 ICUs from 37 hospitals (29 public and 8 private) enrolled in the network, of which 33 were teaching hospitals with >500 beds. The network recorded 679,109 patient days, 212,081 central-line days, and 387,092 urinary catheter days. Overall, 4,301 bloodstream infection (BSI) events and 1,402 urinary tract infection (UTI) events were reported. The network CLABSI rate was 9.4 per 1,000 central-line days and the CAUTI rate was 3.4 per 1,000 catheter days. The central-line utilization ratio was 0.31 and the urinary catheter utilization ratio was 0.57. Moreover, 3,542 (73%) of 4,742 pathogens reported from BSIs and 868 (53%) of 1,644 pathogens reported from UTIs were gram negative. Also, 1,680 (26.3%) of all 6,386 pathogens reported were Enterobacteriaceae. Of 1,486 Enterobacteriaceae with complete antibiotic susceptibility testing data reported, 832 (57%) were carbapenem resistant. Of 951 Enterobacteriaceae subjected to colistin broth microdilution testing, 62 (7%) were colistin resistant. The surveillance platform identified 2 separate hospital-level HAI outbreaks; one caused by colistin-resistant K. pneumoniae and another due to Burkholderia cepacia. Phased expansion of surveillance to additional hospitals continues. Conclusions: HAI surveillance was successfully implemented across a national network of diverse hospitals using modified NHSN protocols. Surveillance data are being used to understand HAI burden and trends at the facility and national levels, to inform public policy, and to direct efforts to implement effective hospital IPC activities. This network approach to HAI surveillance may provide lessons to other countries or contexts with limited surveillance capacity.

Funding: None

Disclosures: None

Garra gotyla is an indigenous coldwater fish of the cyprinid family and has wide geographical distribution in India as well as in other countries of Asia and Africa. Induced breeding in G. gotyla was carried out successfully for the first time and an attempt has been made to document developmental stages chronologically from the first minute of fertilization, through all stages of embryonic development until the fifth day post hatching. This experiment was carried out at 22–24°C water temperature at the Directorate of Coldwater Fisheries Research, Bhimtal, India. During the breeding trial, the fertilization rate was observed as 70–75% and hatching rate was 85–90%. The mature fertilized ova were measured as 0.8–1.0 mm in diameter and the perivitelline membrane became thick soon after fertilization and formation of the germ pole. The periods taken for complete developmental stages were recorded; cleavage stage 111 min (min post fertilization (pf)), blastulation stage 580 min (pf), neurulation and segmentation 1250 min (pf) and hatching was completed after 1420 min. The sac fry was measured as 3 mm in length and took almost 3 days for complete absorption of the yolk content. The major structural and differential changes observed are in head, tail, fins, alimentary canal, rudiments of each organ and appearance of melanophore pigmentation in the whole body. The 5-day-old larvae were measured as 6 mm in length with almost every organ fully differentiated. The present study will be utilized for large-scale production of fingerlings for stock enhancement in rivers, lakes and possibilities of genetic improvement and manipulation at the embryonic stage.

Particle acceleration induced by fast magnetic reconnection may help to solve current puzzles related to the interpretation of the very high energy (VHE) and neutrino emissions from AGNs and compact sources in general. Our general relativistic-MHD simulations of accretion disk-corona systems reveal the growth of turbulence driven by MHD instabilities that lead to the development of fast magnetic reconnection in the corona. In addition, our simulations of relativistic MHD jets reveal the formation of several sites of fast reconnection induced by current-driven kink turbulence. The injection of thousands of test particles in these regions causes acceleration up to energies of several PeVs, thus demonstrating the ability of this process to accelerate particles and produce VHE and neutrino emission, specially in blazars. Finally, we discuss how reconnection can also explain the observed VHE luminosity-black hole mass correlation, involving hundreds of non-blazar sources like Perseus A, and black hole binaries.

We present a detailed analysis of the radio galaxy PKS $2250{-}351$ , a giant of 1.2 Mpc projected size, its host galaxy, and its environment. We use radio data from the Murchison Widefield Array, the upgraded Giant Metre-wavelength Radio Telescope, the Australian Square Kilometre Array Pathfinder, and the Australia Telescope Compact Array to model the jet power and age. Optical and IR data come from the Galaxy And Mass Assembly (GAMA) survey and provide information on the host galaxy and environment. GAMA spectroscopy confirms that PKS $2250{-}351$ lies at $z=0.2115$ in the irregular, and likely unrelaxed, cluster Abell 3936. We find its host is a massive, ‘red and dead’ elliptical galaxy with negligible star formation but with a highly obscured active galactic nucleus dominating the mid-IR emission. Assuming it lies on the local M– $\sigma$ relation, it has an Eddington accretion rate of $\lambda_{\rm EDD}\sim 0.014$ . We find that the lobe-derived jet power (a time-averaged measure) is an order of magnitude greater than the hotspot-derived jet power (an instantaneous measure). We propose that over the lifetime of the observed radio emission ( ${\sim} 300\,$ Myr), the accretion has switched from an inefficient advection-dominated mode to a thin disc efficient mode, consistent with the decrease in jet power. We also suggest that the asymmetric radio morphology is due to its environment, with the host of PKS $2250{-}351$ lying to the west of the densest concentration of galaxies in Abell 3936.

Sustainability of cereal-based cropping systems remains crucial for food security in South Asia. However, productivity of cereal–cereal rotations has declined in the long run, demonstrating the need for a sustainable alternative. Base crop, that is, common crop in different crop rotations, productivity could be used as a sustainability indicator for the assessment of different long-term crop rotations. This study aimed to evaluate the impact of grain legume inclusion in lowland rice–wheat (R-W) and upland maize–wheat (M-W) rotation on system’s base crop (rice in lowland and wheat in upland crop rotations) productivity and sustainability and also in soil fertility. Mung bean (April–May) inclusion in R-W rotation increased rice grain yield by 10–14%. In upland, mung bean inclusion in M-W rotation increased wheat grain yield by 5–11%. Replacing wheat with chickpea in R-W rotation increased rice grain yield by 5–8%. Increased base crop productivity in legume inclusive rotations was attributed to significant improvement in panicle (rice) or spike (wheat) attributes. Increased soil organic carbon and available nitrogen and phosphorus in the legume inclusive rotations significantly influenced the base crop productivity in both the production systems. Among the crop rotations, R-W-Mb (in lowland) and M-W-Mb (in upland) rotations had the highest system productivity and net return. Therefore, intensification/diversification of cereal–cereal rotations with grain legume could improve soil fertility and sustain crop productivity.

Glaciological ablation is computed from point-scale data at a few ablation stakes that are usually regressed as a function of elevation and averaged over the area-elevation distribution of a glacier. This method is contingent on a tight control of elevation on local ablation. However, in debris-covered glaciers, systematic and random spatial variations of debris thickness modify the ablation rates. We propose and test a method to compute sub-debris ablation where stake data are interpolated as a function of debris-thickness alone and averaged over the debris-thickness distribution at different parts of the glacier. We apply this method on Satopanth Glacier located in Central Himalaya utilising ~1000 ablation measurements obtained from a network of up to 56 stakes during 2015–2017. The estimated mean sub-debris ablation ranges between 1.5±0.2 to 1.7±0.3 cm d−1. We show that the debris-thickness-dependent regression describes the spatial variability of the sub-debris ablation better than the elevation dependent regression. The uncertainties in ablation estimates due to the corresponding uncertainties in the measurement of ablation and debris-thickness distribution, and those due to interpolation procedures are estimated using Monte Carlo methods. Possible biases due to a finite number of stakes used are also investigated.

Severe acute malnutrition (SAM) is a major cause of child mortality and morbidity. Children treated for SAM are at risk of refeeding hypophosphataemia (HP). The study was done to find out the incidence and various predictors of moderate/severe HP in SAM among North Indian children. This prospective observational study was conducted from August 2014 to July 2015 in the inpatients’ department of Department of Paediatrics at King George’s Medical University, Lucknow, Uttar Pradesh, North India, a tertiary care teaching hospital. Before inclusion, ethical approval and written informed consent was obtained. Included in the study were sixty-five children aged 6–59 months of age, who were admitted to the hospital with SAM as per the WHO guidelines. SAM was defined as a mid-upper arm circumference <115 mm and/or weight-for-height/length <–3 z-scores of the WHO growth standards and/or have bilateral oedema. Serum P levels were measured on admission and for five consecutive days after starting feed. HP was defined as mild, moderate and severe with a cut-off of 1·19–0·65, 0·65–0·32 and <0·32 mmol/l, respectively. About 60 % children had HP, with 20 % having moderate/severe HP at admission. The proportion of HP increased to 83·1 %, with 38·5 % having moderate/severe HP on day 3 after feeding. It was concluded that HP is a common biochemical abnormality in SAM. Considering its impact on the health of the child, serum P levels should be monitored in patients with SAM who are treated with enteral feeding.

Solar rotation is still one of the unresolved concern of solar physics. We performed time series analysis on the bins formed on equally separated latitude regions on the soft X-ray images. These images are observed with the X-ray telescope (XRT) on board the Hinode satellite. The flux modulation method traces the passage of X-ray feature over the solar disc and statistical analysis of the time series data of the SXR images (one per day) for the period extends from year 2015 to 2017 gives the coronal rotation period as a function of latitude. The investigation provided quite systematic information of the solar rotation and its variability.

Bovine calf scours reported to be caused by multiple aetiologies resulting in heavy mortality in unweaned calves and huge economic loss to the dairy farmers. Among these, cryptosporidiosis is an emerging waterborne zoonoses and one of the important causes of neonatal calf diarrhoea. Poor immune response coupled with primary cryptosporidial infections predispose neonatal calves to multiple secondary infections resulting in their deaths. In the present study, faecal samples from 100 diarrhoeic calves randomly picked up out of 17 outbreaks of bovine calf diarrhoea in periurban Ludhiana, Punjab in Northern India were subjected to conventional (microscopy, modified Zeihl–Neelsen (mZN) staining) and immunological and molecular techniques (faecal antigen capture ELISA and PCR) for detection of primary Cryptosporidium parvum infection as well as other frequently reported concurrent pathogens, viz. rotavirus and coronavirus, Salmonella spp., Escherichia coli, Clostridium perfringens and Eimeria spp. The faecal antigen capture ELISA and PCR revealed 35% prevalence of C. parvum in contrast to 25% by mZN staining with a relatively higher prevalence (66·7%) in younger (8–14-day-old) calves. The detection rate of the other enteropathogens associated with C. parvum was 45·71% for C. perfringens followed by Salmonella spp (40·0%), rotavirus (36·0%), coronavirus (16·0%), E. coli (12·0%) and Eimeria spp (4·0%) The sensitivity for detection of C. parvum by ELISA and mZN staining in comparison to PCR was 97·14% and 72·72%, respectively. An important finding of the study was that C. parvum alone was found in only 10% of the diarrhoeic faecal samples, whereas, majority of the samples (90%) showed mixed infections ranging from a combination of two to five agents. This is the first documentary proof of C. parvum and associated pathogens responsible for severe periurban outbreaks of bovine calf diarrhoea culminating in heavy mortality from Northern India.

Avalanching is a prominent source of accumulation on glaciers that have high and steep valley-walls surrounding their accumulation zones. These glaciers are typically characterised by an extensive supraglacial debris cover and a low accumulation area ratio. Despite an abundance of such glaciers in the rugged landscapes of the High Himalaya, attempts to quantify the net avalanche contribution to mass balance and its long-term variation are almost missing. We first discuss diagnostic criteria to identify strongly avalanche-fed glaciers. Second, we develop an approximate method to quantify the magnitude of the avalanche accumulation exploiting its expected control on the dynamics of these glaciers. The procedure is based on a simplified flowline model description of the glacier concerned and utilises the known glaciological mass-balance, velocity and surface-elevation profiles of the glacier. We apply the method to three Himalayan glaciers and show that the data on the recent dynamics of these glaciers are consistent with a dominant contribution of avalanches to the total accumulation. As a control experiment, we also simulate another Himalayan glacier where no significant avalanche contribution is expected, and reproduce the recent changes in that glacier without any additional avalanche contribution.

The possibilities of electron acceleration by ponderomotive force of a circularly polarized laser pulse in magnetized quantum plasma have been explored. The basic mechanism involves acceleration of electron by the axial gradient in the ponderomotive potential of the laser. The quantum effects have been taken into account for a high-density plasma. The ponderomotive force of the laser is resonantly enhanced when Doppler up-shifted laser frequency equals the cyclotron frequency.

We present a study performed to understand the role of magnetic field during a historical outburst of IBL S5 0716+714 witnessed in early 2015. The two month long profile of the outburst reveals several episodes of sub-flares superimposed over the diminishing trend of the peak-flux. The broadband X-ray spectrum from Swift + Nustar exhibits a break at 4.9+0.4−0.5 keV which is consistent with the valley in the SED predicted by model. The spectral index variations, closely correlated to the flux variations during the first prominent sub-flare, indicate the alterations in the particle energy distribution coinciding the onset of the flare. The PA rotations approximately at the same epoch are used to constrain the field and its geometry.

Medicinal plants are the vital source of numerous structurally diverse pharmacologically active metabolites collectively called as secondary metabolites finding extensive applications in traditional systems of medicine and in pharmaceutical industries. Several distinctive and complex pathways operate in an interactive manner via metabolic networks that are responsible for the accumulation of such highly specialized metabolites. Secondary metabolites are believed to play a wide spectrum of physiological and functional roles in plants, many of which being investigated and supported by the experimental studies. Biosynthetic pathway related studies on various aspects in these medicinal plants have been found very tedious owing to several issues in plants such as considerably lower metabolite concentrations in native tissues, existence at different locations, and highly complex multi-step pathways, etc. Pathway elucidation and gene/enzyme discovery for studying metabolic pathway evolution and subsequent engineering could be better achieved by mining various pathway databases and reconstruction of metabolic networks available at different omics databases. Though medicinal plants have a limited range of genomic sequences available, however recently, next generation sequencing is being widely used to generate a comprehensive transcriptomic resource for these plants. It is anticipated that databases and resources generated from these studies are likely to play a key role towards the study and exploitation of metabolites from medicinal plants in near future. In this review, we have discussed next generation sequencing approaches, which were used for the generation of transcriptomic resources for several medicinally important plants. The relevance of transcriptomic approaches in curation of the pathways linked with the synthesis of major secondary metabolites along with their precursors of pharmaceutical importance in medicinal plants is also comprehensively analysed.

The emitted aliphatic hydrocarbon profile of four Indian Coccinellidae (Coleoptera), Coccinella septempunctata (Linnaeus) (C7), Coccinella transversalis Fabricius (Ct), Menochilus sexmaculatus (Fabricius) (Ms), and Propylea dissecta (Mulsant) (Pd) has been investigated by simple solvent-less headspace solid-phase microextraction (HS-SPME) technique coupled with gas chromatography and mass spectroscopy (GC-MS). Identified volatile and non-volatile compounds were confirmed by running corresponding standards and comparing with the National Institute of Standards and Technology library. Among the 56 identified aliphatic hydrocarbons, saturated aliphatic hydrocarbons were more in number than unsaturated ones. Among saturated hydrocarbons, methyl branched hydrocarbons were more in number in C7 and Ct than Ms and Pd. Menochilus sexmaculatus and Pd had higher percentages of unsaturated hydrocarbons than C7 and Ct. Among branched chain-hydrocarbons, mono-methylated saturated hydrocarbons were more in number than dimethylated saturated hydrocarbons. Further analysis of the semiochemical profile revealed a closeness between C7 and Ct, and between Ms and Pd. Quantitative analysis revealed that straight chain hydrocarbons form separate clusters to branched chain methylated hydrocarbons. This is the first attempt to identify the semiochemical profile of some Indian coccinellids using the headspace solid phase micro-extraction technique coupled with the gas chromatography-mass spectrometry technique. This report will be helpful for various chemotaxonomic studies of the species in the future.

A large number of naked, fossil spore tetrads assignable to the dispersed microspore genera Indotriradites, Microbaculispora and Microfoveolatispora are reported for the first time from an early Permian stratum (Lower Barakar Formation) of Singrauli Coalfield, Son-Mahanadi Basin, Central India. This is also the first record of tetrads from any Artinskian strata in the world. There is no evidence of any kind of sporangia or related plant parts in the present investigation that could ascertain the affinity of these tetrads; however, the presence of a trilete mark in the spores of the tetrads demonstrates their alliance at least with the pteridophyte group. The present study suggests possible factors affecting the sporogenesis process in the past, considering other available global records pertaining to fossil spore tetrads. The results of significant physiological and biochemical analyses performed on the anthers of modern plants related to reproductive biology, in order to understand the conditions and changes responsible for the formation of tetrads, are also considered. We analysed the globally occurring fossil tetrads and the palaeoclimates prevailing during their deposition. A correlation between extreme climatic conditions, specific pH values inside microsporangium and the formation of tetrad is envisaged. It is deduced that extreme climatic conditions (extreme cold/extreme hot) might have triggered some sort of malfunctioning in the sporogenesis process that altered the specific pH values inside the microsporangium. Any restraint of the activity of the callase enzyme, responsible for dissolution of callose walls laid between the individual spores, may therefore have apprehended the dissociation of tetrads into individual spores.

Several epidemiological studies have shown that the consumption of finger millet (FM) alleviates diabetes-related complications. In the present study, the effect of finger millet whole grain (FM-WG) and bran (FM-BR) supplementation was evaluated in high-fat diet-fed LACA mice for 12 weeks. Mice were divided into four groups: control group fed a normal diet (10 % fat as energy); a group fed a high-fat diet; a group fed the same high-fat diet supplemented with FM-BR; a group fed the same high-fat diet supplemented with FM-WG. The inclusion of FM-BR at 10 % (w/w) in a high-fat diet had more beneficial effects than that of FM-WG. FM-BR supplementation prevented body weight gain, improved lipid profile and anti-inflammatory status, alleviated oxidative stress, regulated the expression levels of several obesity-related genes, increased the abundance of beneficial gut bacteria (Lactobacillus, Bifidobacteria and Roseburia) and suppressed the abundance of Enterobacter in caecal contents (P≤ 0·05). In conclusion, FM-BR supplementation could be an effective strategy for preventing high-fat diet-induced changes and developing FM-BR-enriched functional foods.

The Diguva Sonaba area (Vishakhapatnam district, Andhra Pradesh, South India) represents part of the granulite-facies terrain of the Eastern Ghats Mobile Belt. The Precambrian metamorphic rocks of the area predominantly consist of mafic granulite (±garnet), khondalite, leptynite (±garnet, biotite), charnockite, enderbite, calc-granulite, migmatic gneisses and sapphirine–spinel-bearing granulite. The latter rock type occurs as lenticular bodies in khondalite, leptynite and calc-granulite. Textural relations, such as corroded inclusions of biotite within garnet and orthopyroxene, resorbed hornblende within pyroxenes, and coarse-grained laths of sillimanite, presumably pseudomorphs after kyanite, provide evidence of either an earlier episode of upper-amphibolite-facies metamorphism or they represent relics of the prograde path that led to granulite-facies metamorphism. In the sapphirine–spinel-bearing granulite, osumilite was stable in addition to sapphirine, spinel and quartz during the thermal peak of granulite-facies metamorphism but the assemblage was later replaced by Crd–Opx–Qtz–Kfs-symplectite and a variety of reaction coronas during retrograde overprint. Variable amounts of biotite or biotite+quartz symplectite replaced orthopyroxene, cordierite and Opx–Crd–Kfs–Qtz-symplectite at an even later retrograde stage. Peak metamorphic conditions of c. 1000°C and c. 12 kbar were computed by isopleths of XMg in garnet and XAl in orthopyroxene. The sequence of reactions as deduced from the corona and symplectite assemblages, together with petrogenetic grid and pseudosection modelling, records a clockwise P–T evolution. The P–T path is characteristically T-convex suggesting an isothermal decompression path and reflects rapid uplift followed by cooling of a tectonically thickened crust.