The use of forage as a cover crop is an alternative for the sustainable management of conilon coffee (Coffea canephora Pierre ex Froehner) crops. The objective of this study was to evaluate the herbage accumulation and nutritive value of forages used as cover crops and their effect on the productivity and physiology of conilon coffee plants. The inter-row management assessed were 1- Congo grass [Urochloa ruziziensis (R. Germ. & C.M. Evrard) Crins], 2- Mombaça guineagrass [Megathyrsus maximus (Jacq.) B.K. Simon & S.W.L. Jacobs], 3- Marandu palisadegrass [Urochloa brizantha (Hochst. ex A.Rich.) R.D.Webster], 4- weeds, 5- weeding and herbicide application. The experiment was conducted in 2020 and 2021 using a randomized block design (split-plot) with four replications and a plot size of 24 m2. Herbage accumulation of Congo grass, Mombaça guineagrass and Marandu palisadegrass (1.12 to 3.81 t/ha) were higher than weeds (0.18 to 1.95 t/ha) in seven periods evaluated. Mombaça guineagrass had the highest average herbage accumulation (1.47 to 3.81 t/ha). The forage cover crops did not differ among themselves for dry matter concentration, crude protein and C:N ratio in three periods evaluated. The inter-rows management with cover crops did not reduce productivity, grain/fruit ratio, grain size, vegetative vigour and physiology of the coffee plants compared to the management with weeding and herbicide in 2021. In 2022, they stagnated or reduced productivity by up to 49%, with changes in plant physiology. Adjustments in the management of cover crops are needed for the development of competitive and sustainable coffee crops.

The aim of this study is to assess the effects of substituting soybean meal with extruded urea in the diet of crossbred Texel x no defined racial pattern lambs under continuous grazing on Brachiaria ssp., focusing on both their productive and nutritional performance. 60 Texel crossbred lambs (12 animals for each treatment) were used, with an average initial weight of 20.7 ± 0.87 kg and an average age of 2.5 ± 0.70 months, fed treatments with increasing levels of UE (Urea extruded Amireia® 200S): 0; 6; 12; 18 and 24 grams of EU 100/kg of body weight, with trial period was 5 months, using the multivariate technique. The data were subjected to principal component and canonical discriminant analysis to check possible differences between the evaluated treatments and identify the variables that best discriminate and use these variables to create a discriminant function that represents the differences between treatments. Of the 12 variables initially used, we observed that 9 were used by the main components, but 6 were those that presented the greatest discriminatory power for the study. Main component 1 was characterized by biometric measurements and showed the greatest power of variation in the study (60%), followed by main component 2, represented by slaughter weight and empty body weight (13%). These correlations indicate that biometric measurements can serve as reliable indirect indicators for estimating carcass traits in sheep, offering a practical alternative to visual assessments.

The study evaluated forage and livestock performance in different grazing systems over two years. Treatments were three contrasting grazing systems: (I) N-fertilized bahiagrass (Paspalum notatum Flüggé) in the summer overseeded during the winter by N-fertilized ryegrass (Lolium multiflorum) and oat (Avena sativa L.) (Grass + N); (II) unfertilized bahiagrass during the summer overseeded with ryegrass + oat and a blend of clovers (Trifolium spp.) in the winter (Grass + Clover); (III) unfertilized bahiagrass and rhizoma peanut (RP; Arachis glabrata Benth.) mixture during summer, overseeded during winter by ryegrass + oat + clovers mixture (Grass + Clover + RP). Average daily gain (ADG), gain per area (GPA), and stocking rate (SR) in the winter did not differ across treatments and averaged 0.87 kg/d (P = 0.940), 303 kg/ha, and 2.72 AU/ha. In the summer, Grass + Clover + RP had greater ADG than Grass + N (0.34 vs. 0.17 kg/d, respectively). During the summer, the GPA of Grass + Clover + RP was superior to Grass + N (257 vs. 129 kg/ha, respectively), with no difference in SR among treatments at 3.19 AU/ha. Over the entire year, ADG and GPA tended to be greater for Grass + Clover + RP. Annual SR differed between treatments, where Grass + N was greater (3.37 AU/ha) than the other treatments, which averaged 2.76 AU/ha. Integration of legumes into pasture systems in the summer and winter contributes to developing a sustainable grazing system, reducing N fertilizer use by 85% while tending to increase livestock productivity even though SR was decreased by 18%.

Congenital transmission of Chagas disease plays an important role in endemic countries because it is not a diagnosis that is encountered frequently in prenatal care. Due to limited information regarding congenital transmission of Trypanosoma cruzi in Mexico, the present study aimed to investigate protozoan infectivity and modulation of immune responses in human placental explants infected with T. cruzi Ia Mexican strains. The Inc-5 strain showed increased infectivity and modulated IL-1β, IL-10 and TLR-4, decreasing their expression after 24 h of infection. Both strains (Inc-5 and Ninoa) stimulated the production of TNF-α and decreased IL-6 levels 96 h after infection. An important detachment of the syncytiotrophoblast caused by infection with T. cruzi was observed after 24 h of infection. In this study, ex vivo infection of human placental villi was performed to better understand interactions involving parasitic T. cruzi and human placental tissue. It was concluded that the strains of TcIa present parasitism in placental tissue, modulation of the innate immune system of the placenta, and cause intense detachment of the syncytiotrophoblast, a fact that may be more associated with abortion and premature birth events than the congenital transmission itself, justifying the low rate of this transmission mechanism by this genotype.

Green tea is a popular drink used for therapeutic purposes to mitigate the consequences of diabetes. In this study, we aimed at evaluating the potential of green tea infusion to ameliorate structural and enzymatic damages caused by hyperglycemia in the testis and epididymis of Wistar rats. For that, nondiabetic and streptozotocin-induced diabetic rats (negative control and diabetes control, respectively) received 0.6 mL of water by gavage. Another set of diabetic animals received 100 mg/kg of green tea infusion diluted in 0.6 mL of water/gavage (diabetes + green tea) daily. After 42 days of treatment, the testes and epididymides were removed and processed for histopathological analysis, micromineral determination, and enzymatic assays. The results showed that treatment with green tea infusion preserved the testicular and epididymal histoarchitecture, improving the seminiferous epithelium and the sperm production previously affected by diabetes. Treatment with green tea reduced tissue damages caused by this metabolic condition. Given the severity of hyperglycemia, there was no efficacy of the green tea infusion in maintaining the testosterone levels, antioxidant enzyme activity, and microminerals content. Thus, our findings indicate a protective effect of this infusion on histological parameters, with possible use as a complementary therapy for diabetes.

The present study aimed to evaluate the inflammatory response, oxidative status and fatty acid deposition in reproductive tissues of cats supplemented with the dried microalgae Schizochytrium spp. (Thraustochytriaceae) as a DHA source. Thirty-seven cats (males, n 21; females, n 16; 11·5 (sd 0·5) months of age) were divided by sex into five groups. Treatment diets contained algae biomass at 4·0, 8·0, 12·0 or 16·0 g/kg replacing poultry fat (n-6 source). Cats were fed the respective diet for 62 d and neutered on day 58. Blood samples were collected at the beginning of the experiment (day 1), before neutering (day 58) and 4 d after surgery (day 62) for analysis of inflammation and oxidative markers. Acute-phase protein levels were altered (P < 0·01) in the postoperative period, without any treatment effect (P > 0·05). PGE2 concentrations after surgery were reduced linearly (R2 0·8706; P = 0·002) with microalgal inclusion. Blood platelet count was reduced (P = 0·001) after the surgery regardless treatment, but it was higher in the DHA group compared with control (P < 0·001). The DHA deposition (testicles, R2 0·846; ovaries, R2 0·869) and the n-6:n-3 ratio (testicles, R2 0·859; ovaries, R2 0·955) in gonads had a pattern which fitted a quadratic model. DHA from Schizochytrium spp. modifies PGE2 response after the surgery in cats. The physiological roles of the DHA in the reproduction of cats were not investigated, but its gonadal deposition after supplementation was observed.

Denture-related stomatitis caused by Candida spp. affects elderly individuals using partial/total prosthesis, provoking several discomforts including burning sensation and altered taste. Herein, we have studied 52 denture-wearing individuals (>60 years-old), attended at the dentistry clinic of UNIVALE, aiming to isolate Candida spp. directly from the stomatitis lesions and to evaluate their potential to produce virulence attributes. A low prevalence of denture-related stomatitis was reported in these patients (4/52; 7.7%). Candida albicans was isolated in the 4 selected patients, with the ability to form biofilm over a polystyrene surface and to produce aspartic protease, esterase and hemolysin. However, neither phospholipase nor caseinase activities were detected. Planktonic-growing yeasts were susceptible to amphotericin B and caspofungin, while the susceptibility to azoles (fluconazol, itraconazole and voriconazole) varied depending on either the isolate or antifungal. Relevantly, biofilm-forming C. albicans cells exhibited resistance to all studied antifungals. So, new effective drugs against resistant C. albicans isolates causing denture-related stomatitis are urgently required.

Isotopic composition of leaf carbon (δ13C) and nitrogen (δ15N) is determined by biotic and abiotic factors. In order to determine the influence of leaf habit and site on leaf δ13C and δ15N in the understorey of two Atlantic forests in Brazil that differ in annual precipitation (1200 and 1900 mm), we measured these isotopes in the shaded understorey of 38 tropical tree species (20 in the 1200-mm site and 18 in the 1900-mm site). Mean site values for δ15N were significantly lower at the 1200-mm site (−1.4‰) compared with the 1900-mm site (+3.0‰), and δ13C was significantly greater in the 1200-mm site (−30.4‰) than in the 1900-mm site (−31.6‰). Leaf C concentration was greater and leaf N concentration was lower at 1200-mm than at 1900-mm. Leaf δ15N was negatively correlated with δ13C across the two sites. Leaf δ13C and δ15N of evergreen and deciduous species were not significantly different within a site. No significant phylogenetic signal for any traits among the study species was found. Overall, site differences were the main factor distinguishing traits among species, suggesting strong functional convergence to local climate and soils within each site for individuals in the shaded understorey.

The African buffalo (Syncerus caffer), a mammal species whose population is declining, can play a role as a reservoir or carrier of a wide number of arthropod-borne pathogens. Translocation procedures have been used as an alternative approach for species conservation. However, the veterinary aspects of this sort of procedures are extremely important to minimize the impact on animal health. In order to detect Bartonella and haemoplasmas, two important group of bacterial that have an impact in both human and animal health, EDTA whole-blood samples were screened for the presence of these bacterial pathogens by molecular techniques. As a result, a molecular occurrence of 4.1 and 15.4% for Bartonella spp. and haemoplasmas, respectively, was reported among 97 wild buffaloes sampled during a translocation procedure from Marromeu to Gorongosa Reserve, Mozambique. Additionally, phylogenetic analyses of the obtained sequences were conducted. At least, three bovine-associated pathogens, namely B. bovis, M. wenyonii and ‘Candidatus M. haemobos’, as well as a probably new Bartonella genotype/species were detected in S. caffer. Further studies are needed in order to determine whether these bacterial species may cause impact in buffaloes and other sympatric ruminant species living in the release site.

Eucalyptus spp. is the main tree used in cellulose production worldwide, cultivated mostly in extensive monocrop plantations. However, due to concerns on environmental impacts, fear on decrease of food production related to the size of cultivated area, its location near human settlements with few jobs generation, eucalypt cultivation is no longer encouraged in many places. At the same time, large plantations hold the opportunity to integrate eucalypt into agroforestry systems (AFS) in a variety of production systems with many social, economical and environmental advantages. In this study we studied canopy modifications of E. camaldulensis sprouts under different degrees of shade. The plants were located on a gradient of available solar irradiation ranging from 51 to 94%. E. camaldulensis showed canopy plasticity with modified radiation interception patterns under diverse irradiations. Most of these variations were of small amplitude with some important variables remaining almost unchanged (leaf density, canopy percentage, tree, trunk and canopy height) or increasing only slightly (leaf area index and canopy opening). The main changes presented by E. camaldulensis, with a steep increase towards full sun, were: foliage area, canopy surface, canopy volume and area of canopy projection. In order to design appropriate agroforestry systems with young eucalypt growing under the shade of other crops, it is necessary to determine at which point the observed variable changes can support reasonable production.

Shape memory alloys are functional materials that can recover plastic strains between 2 and 6%. This property can be used to produce actuators for many areas as medicine, robotic, aeronautic and others. Recently, it has been observed the particular interest for shape memory alloys welding, especially to obtain Ni-Ti similar and dissimilar joints and fabricate simple or complex structures. In this sense, this work present an experimental study of tungsten inert gas pulsed welding applied to Ni-Ti shape memory alloy wires with 0.9 mm in diameter, previously heat treated at 450 °C for 20 minutes and air cooled. For that, it was carried out tensile tests at isothermal temperatures from 40 °C to 90 °C (steps of 10 °C) for welded and unwelded wires. The transformation temperatures obtained from differential scanning calorimetry were compared to verify the effect of welding process. It was also performed a stabilization process by mechanical cycling in some welded and unwelded Ni-Ti wires. The results showed a low strength and strain capacity of the weld joint at higher temperatures. Although, at lowest temperature, close to 40 °C, it was observed higher values of maximum stress and strain for welded Ni-Ti wires.

Leishmaniasis is a vector-borne disease and an important public health issue. Glycosaminoglycan ligands in Leishmania parasites are potential targets for new strategies to control this disease. We report the subcellular distribution of heparin-binding proteins (HBPs) in Leishmania (Viannia) braziliensis and specific biochemical characteristics of L. (V.) braziliensis HBPs. Promastigotes were fractionated, and flagella and membrane samples were applied to HiTrap Heparin affinity chromatography columns. Heparin-bound fractions from flagella and membrane samples were designated HBP Ff and HBP Mf, respectively. Fraction HBP Ff presented a higher concentration of HBPs relative to HBP Mf, and SDS-PAGE analyses showed 2 major protein bands in both fractions (65 and 55 kDa). The 65 kDa band showed gelatinolytic activity and was sensitive to inhibition by 1,10-phenanthroline. The localization of HBPs on the promastigote surfaces was confirmed using surface plasmon resonance (SPR) biosensor analysis by binding the parasites to a heparin-coated sensor chip; that was inhibited in a dose-dependent manner by pre-incubating the parasites with variable concentrations of heparin, thus indicating distinct heparin-binding capacities for the two fractions. In conclusion, protein fractions isolated from either the flagella or membranes of L. (V.) braziliensis promastigotes have characteristics of metallo-proteinases and are able to bind to glycosaminoglycans.

Very little is known about the physiological interactions between plant hosts and symptomless endophytic fungi despite their widespread occurrence. We investigated the impact of two such fungi, Colletotrichum musae and Fusarium moniliforme, upon the photosynthetic capacity of two crop plants, banana and maize, respectively. Endophyte-free plants were obtained first and then infected with the fungi. Measurements of total chlorophyll content revealed very little difference between endophyte-free and infected plants of banana, whereas in maize they showed 50% reductions in the endophyte-infected plants. The maximum photochemical capacity (Fv/Fm ) was measured in order to determine if the plants had any photoinhibitory effect caused by biotic or abiotic factors. After 45 d of growth, endophyte-free banana plants had similar values of Fv/Fm to plants typical of nonstressed conditions, whereas the endophyte-infected plants showed a reduction of approx. 15%. Unlike banana, infected maize plants displayed values of Fv/Fm similar to those of control and endophyte-free plants, indicating that the maximum photochemical capacity was not affected by infection. The light response curves of both species showed that the photosynthethic capacity was severely reduced in endophyte-infected plants, reaching saturation at c. 400 μmol m−2s−1 whereas the control and endophyte-free plants were saturated at much higher photon flux densities. In banana the effect seemed to be due to an impairment of electron transport in the thylakoid membranes. By contrast, reduction of the photosynthetic capacity in maize was due to a reduction in chlorophyll content, leading to a decrease in the electron transport components and a consequent reduction in carbohydrate synthesis. It is possible that the reduction in the maximum yield of photosynthesis in both crops was caused by toxins produced by the fungi. Nevertheless there were no major macroscopic effects on the plants to indicate disease symptoms.