Background: Despite a higher prevalence of traumatic spinal cord injury (TSCI) amongst Canadian Indigenous peoples, there is a paucity of studies focused on Indigenous TSCI. We present the first Canada-wide study comparing TSCI amongst Canadian Indigenous and non-Indigenous peoples. Methods: This study is a retrospective analysis of prospectively-collected TSCI data from the Rick Hansen Spinal Cord Injury Registry (RHSCIR) from 2004-2019. We divided participants into Indigenous and non-Indigenous cohorts and compared them with respect to demographics, injury mechanism, level, severity, and outcomes. Results: Compared with non-Indigenous patients, Indigenous patients were younger, more female, less likely to have higher education, and less likely to be employed. The mechanism of injury was more likely due to assault or transportation-related trauma in the Indigenous group. The length of stay for Indigenous patients was longer. Indigenous patients were more likely to be discharged to a rural setting, less likely to be discharged home, and more likely to be unemployed following injury. Conclusions: Our results suggest that more resources need to be dedicated for transitioning Indigenous patients sustaining a TSCI to community living and for supporting these patients in their home communities. A focus on resources and infrastructure for Indigenous patients by engagement with Indigenous communities is needed.

Plasmodium knowlesi has risen in importance as a zoonotic parasite that has been causing regular episodes of malaria throughout South East Asia. The P. knowlesi genome sequence generated in 2008 highlighted and confirmed many similarities and differences in Plasmodium species, including a global view of several multigene families, such as the large SICAvar multigene family encoding the variant antigens known as the schizont-infected cell agglutination proteins. However, repetitive DNA sequences are the bane of any genome project, and this and other Plasmodium genome projects have not been immune to the gaps, rearrangements and other pitfalls created by these genomic features. Today, long-read PacBio and chromatin conformation technologies are overcoming such obstacles. Here, based on the use of these technologies, we present a highly refined de novo P. knowlesi genome sequence of the Pk1(A+) clone. This sequence and annotation, referred to as the ‘MaHPIC Pk genome sequence’, includes manual annotation of the SICAvar gene family with 136 full-length members categorized as type I or II. This sequence provides a framework that will permit a better understanding of the SICAvar repertoire, selective pressures acting on this gene family and mechanisms of antigenic variation in this species and other pathogens.

Conjugated polymers have been proposed as promising materials for scaffolds in tissue engineering applications. However, the restricted processability and biodegradability of conjugated polymers limit their use for biomedical applications. Here we synthesized a block-co-polymer of aniline tetramer and PCL (AT–PCL), and processed it into fibrous non-woven scaffolds by electrospinning. We showed that fibronectin (Fn) adhesion was dependent on the AT–PCL oxidative state, with a reduced Fn unfolding length on doped membranes. Furthermore, we demonstrated the cytocompatibility and potential of these membranes to support the growth and osteogenic differentiation of MC3T3-E1 cells over 21 days.

A paleoclimatic record for a southern Great Plains locality (the Aubrey Clovis site in north-central Texas) has been established using stable carbon and oxygen isotopes. Detailed composite stratigraphic sections, constrained by 14 C ages, place the age of these deposits between 14,200 and 1600 yr B.P. Calcium carbonate samples of lacustrine and pedogenic origin were analyzed. Oxygen isotopic compositions of most of these in situ carbonates reflect equilibrium precipitation from local meteoric waters. Oxygen isotope values reflect changes in the composition of meteoric waters tied to changes in the isotopic composition of moisture derived from the Gulf of Mexico. Oxygen isotopic variability at the Aubrey site is coincident with marine isotopic records from the gulf that vary due to changes in Laurentide ice sheet volume and meltwater influx. The stable carbon isotopic record, reflecting changing biomass through time, corroborates humid versus arid interpretations based on sedimentology and rates of alluviation. A middle Holocene arid period was in contrast to moist early and late Holocene climate, affirming interpretations of other workers studying southern Great Plains Holocene climate history.

We describe the performance of the Boolardy Engineering Test Array, the prototype for the Australian Square Kilometre Array Pathfinder telescope. Boolardy Engineering Test Array is the first aperture synthesis radio telescope to use phased array feed technology, giving it the ability to electronically form up to nine dual-polarisation beams. We report the methods developed for forming and measuring the beams, and the adaptations that have been made to the traditional calibration and imaging procedures in order to allow BETA to function as a multi-beam aperture synthesis telescope. We describe the commissioning of the instrument and present details of Boolardy Engineering Test Array’s performance: sensitivity, beam characteristics, polarimetric properties, and image quality. We summarise the astronomical science that it has produced and draw lessons from operating Boolardy Engineering Test Array that will be relevant to the commissioning and operation of the final Australian Square Kilometre Array Path telescope.

This paper describes the system architecture of a newly constructed radio telescope – the Boolardy engineering test array, which is a prototype of the Australian square kilometre array pathfinder telescope. Phased array feed technology is used to form multiple simultaneous beams per antenna, providing astronomers with unprecedented survey speed. The test array described here is a six-antenna interferometer, fitted with prototype signal processing hardware capable of forming at least nine dual-polarisation beams simultaneously, allowing several square degrees to be imaged in a single pointed observation. The main purpose of the test array is to develop beamforming and wide-field calibration methods for use with the full telescope, but it will also be capable of limited early science demonstrations.

We present results from numerical simulations conducted to investigate a potential method for realizing the required fusion fuel heating in the fast ignition scheme to achieving inertial confinement fusion. A comparison will be made between collisionless and collisional particle-in-cell simulations of the relaxation of a non-thermal electron beam through the two-stream instability. The results presented demonstrate energy transfer to the plasma ion population from the laser-driven electron beam via the nonlinear wave–wave interaction associated with the two-stream instability. Evidence will also be provided for the effects of preferential damping of competing instabilities such as the Weibel mode found to be detrimental to the ion heating process.

Between April and August 2005 Christchurch, New Zealand experienced an outbreak of Legionnaires' disease. There were 19 laboratory-confirmed case including three deaths. Legionella pneumophila serogroup 1 (Lpsg1) was identified as the causative agent for all cases. A case-control study indicated a geographical association between the cases but no specific common exposures. Rapid spatial epidemiological investigation confirmed the association and identified seven spatially significant case clusters. The clusters were all sourced in the same area and exhibited a clear anisotropic process (noticeable direction) revealing a plume effect consistent with aerosol dispersion from a prevailing southwesterly wind. Four out of five cases tested had indistinguishable allele profiles that also matched environmental isolates from a water cooling tower within the centre of the clusters. This tower was considered the most probable source for these clusters. The conclusion would suggest a maximum dispersal distance in this outbreak of 11·6 km. This work illustrated the value of geostatistical techniques for infectious disease epidemiology and for providing timely information during outbreak investigations.

We have tracked the proper motions of ground-state λ7mm SiO maser emission excited by radio Source I in the Orion BN/KL region. Based on dynamical arguments, Source I is believed to be a hard 20 M⊙ binary. The SiO masers trace a linear bipolar outflow (NE-SW) 100 to 1000 AU from the binary. The median 3D velocity is 18 km s−1. An overlying distribution of 1.3 cm H2O masers betrays similar characteristics. The outflow is aligned with the rotation axis of an edge-on disk and wide angle flow known inside 100 AU. Gas dynamics and emission morphology traced by masers around Source I provide dynamical evidence of a magnetocentrifugal disk-wind around this massive YSO, notably a measured gradient in line-of-sight velocity perpendicular to the flow axis, in the same direction as the disk rotation and with comparable speed. The linearity of the flow, despite the high proper motion of Source I and the proximity of dense gas associated with the Orion Hot Core, is also more readily explained for a magnetized flow. The extended arcs of ground-state maser emission bracketing Source I are a striking feature, in particular since dust formation occurs at smaller radii. We propose that the arcs mark two C-type shocks at the transition radius to super-Alfvénic flow.

Despite a significant public health burden the epidemiology of human Campylobacter infection remains blurred. The identification of demographic determinants for Campylobacter infection is therefore essential for identifying potential areas for intervention. Demographic data from an active, population-based sentinel surveillance system for Campylobacter infection (from 2000 until 2003, n=15 907) were compared with appropriate denominator data from the 2001 United Kingdom Census. Incidence was higher in males from birth until the late teens and in females from 20 to 36 years. Age- and gender-specific differences in Campylobacter incidence were observed in different ethnic and socioeconomic groups and hence are all major drivers for Campylobacter infection. Epidemiological studies on Campylobacter infection need to take these factors into consideration during design and analysis. The collation of detailed epidemiological data and its comparison with appropriate denominator data provides a valuable epidemiological tool for studying infection.

A comprehensive picture of high-mass star formation has remained elusive, in part because examples of high-mass young stellar objects (YSOs) tend to be relatively distant, deeply embedded, and confused with other emission sources. These factors have impeded dynamical investigations within tens of AU of high-mass YSOs—scales that are critical for probing the interfaces where outflows from accretion disks are launched and collimated. Using observations of SiO masers obtained with the Very Large Array (VLA) and the Very Long Baseline Array (VLBA), the KaLYPSO project is overcoming these limitations by mapping the structure and dynamical/temporal evolution of the material 10-1000 AU from the nearest high-mass YSO: Radio Source I in the Orion BN/KL region. Our data include ~40 epochs of VLBA observations over a several-year period, allowing us to track the proper motions of individual SiO maser spots and to monitor changes in the physical conditions of the emitting material with time. Ultimately these data will provide 3-D maps of the outflow structure over approximately 30% of the outflow crossing time. Here we summarize recent results from the KaLYPSO project, including evidence that high-mass star formation occurs via disk-mediated accretion.

Studies of antenatal women form the predominant source of data on HIV-1 prevalence in Africa. Identifying factors associated with prevalent HIV is important in targeting diagnostic services and care. Between November 1997 and January 2000, 14 110 postnatal women from Harare, Zimbabwe were tested by ELISAs reactive to both HIV-1 and HIV-2; a subset of positive samples was confirmed with assays specific for HIV-1 and HIV-2. Baseline characteristics were elicited and modelled to identify risk factors for prevalent HIV infection. HIV-1 and HIV-2 prevalences were 32·0% (95% CI 31·2–32·8) and 1·3% (95% CI 1·1–1·5), respectively; 4% of HIV-1-positive and 99% of HIV-2-positive women were co-infected. HIV-1 prevalence increased from 0% among 14-year-olds to >45% among women aged 29–31 years, then fell to <20% among those aged >40 years. In multivariate analyses, prevalence increased with parity, was lower in married women than in single women, divorcees and widows, and higher in women with the lowest incomes and those professing no religion. Adjusted HIV-1 prevalence increased during 1998 and decreased during 1999. Age modified the effects of parity, home ownership and parental education. Among older women, prevalence was greater for women who were not homeowners. Among younger women, prevalence increased with parity and low parental education. None of these factors distinguished women co-infected with HIV-2 from those infected with HIV-1 alone. Prevalent HIV-1 infection is associated with financial insecurity and weak psychosocial support. The ZVITAMBO study apparently spanned the peak of the HIV-1 epidemic among reproductive women in Harare.