Motivated by contradicting or insufficient information regarding the large-scale flow dynamics around surface-mounted finite-height square prisms of small aspect ratio, the present study investigates the dominant vortex shedding and low-frequency dynamics around a surface-mounted cube. These flow modes were obtained from the spectral proper orthogonal decomposition of large-eddy simulation results, at a Reynolds number of $\textit {Re}=1\times 10^4$ and two different types of boundary layer: a thin and laminar boundary layer with thickness $\delta /D=0.2$ and a thick and turbulent boundary layer with $\delta /D=0.8$. The main antisymmetric mode pair revealed a new flow pattern with the alternate shedding of streamwise flow structures, indicating a transition from the half-loops of taller prisms to only streamwise strands (i.e. no vertical core) for smaller aspect ratio. The formation process of the streamwise structures is due to a reorientation of the vorticity of the arch vortex in the streamwise direction characteristic of the shed structures. The low-frequency drift mode affected the length of the recirculation region, the strength of vortex shedding, and the near-wall flow field and pressure distribution on the cube's faces, leading to low-frequency variations in the fluctuating drag and normal force coefficients. These large-scale flow dynamics were similar for both boundary layers, but minor differences were identified, related mostly to the occurrence of flow attachment and the formation of a headband vortex for the thicker boundary layer.

Cyclospora cayetanensis is a coccidian parasite associated with diarrheal illness. In the USA, foodborne outbreaks of cyclosporiasis have been documented almost every year since the mid-1990s. The typical approach used to identify this parasite in human stools is an examination of acid-fast-stained smears under bright-field microscopy. UV fluorescence microscopy of wet mounts is more sensitive and specific than acid-fast staining but requires a fluorescence microscope with a special filter not commonly available in diagnostic laboratories. In this study, we evaluated a new DNA extraction method based on the Universal Nucleic Acid Extraction (UNEX) buffer and compared the performances of four published real-time polymerase chain reaction (PCR) assays for the specific detection of C. cayetanensis in stool. The UNEX-based method had an improved capability to recover DNA from oocysts compared with the FastDNA stool extraction method. The best-performing real-time PCR assay was a C. cayetanensis-specific TaqMan PCR that targets the 18S ribosomal RNA gene. This new testing algorithm should be useful for detection of C. cayetanensis in human stool samples.