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Advancements in high content image analysis have led to an increase in the adoption of these techniques in basic science and clinical research. High-throughput approaches to imaging and image analysis require minimal user interventions, circumventing the often prohibitively time-consuming and unreliable standard manual analysis. In this study, we demonstrate how high content imaging (HCI) techniques, in combination with high content analysis (HCA), can be paired with more traditional manual analysis to quantify both micro- and macro-level features of biopsied tissue sections. High-resolution, full-color images of stained tissue were acquired and stitched together to reconstruct the entire tissue section, which enabled analyses that required accurate identification of a given region's location within the tissue section. A custom region of interest grid was generated that followed the curvature of the tissue. The composite images were used in two separate analyses: tissue layer thickness as a macro-level approach, and nuclei density as a micro-level approach. Ultimately, the flexibility of the HCI and HCA methodologies used in this study allowed for complex analysis of tissue that would not have been otherwise feasible.
Plausible deniability is a common feature of political discourse that allows speakers to avoid taking responsibility for a controversial utterance by invoking possible counter-interpretations. To engage in plausible deniability, Trump invokes seemingly reasonable evidence to contest or refute that he meant what he said, foregrounding only select elements of the meaning-making process while downplaying others. Trump’s use of plausible deniability allows him to rally his base while feigning innocence when confronted about controversial remarks. Although common to politics, Trumpian discourse pushes this language game to the limits of credulity through frequent acts of strategic denying that arouse both supporters and critics. This chapter analyzes the way Trump and his allies engage in plausible deniability through the examination of several high-profile cases, introducing the linguistic concept of “implicature” to shed light on the discursive moves and interpretive processes that underlie attempts to invoke plausible deniability. Key to deconstructing each of these examples is an anthropological understanding of the interactional context in which political remarks are spoken and understood.
As the centennial of the First Red Scare arrives, the time has come to revisit our understanding of it. This methodological article makes the case that the field still struggles with the fundamental problem that the incidents we have collected as the “Red Scare” and “Red Summer” and made national, manifested often as disparate local events that responded to immediate conditions. It argues that responding to the local events of the Red Scare/Red Summer to better understand regional history is not an inadequate response that distracts us from a more worthy attempt to synthesize national currents. Through analyzing smaller-scale strikes and incidents of racial violence, looking at the variance in form and response of local governments, and seeing the global interconnections of the Red Scare through the lens of localities, we can gain new ground toward a broader, more multifaceted understanding of this transformative era.
This article examines the discourse about race and racism that ensued in the US media after the shooting death of an African American youth, Trayvon Martin, by a neighborhood watch volunteer, George Zimmerman, in February 2012. The analysis examines news programs from the three major cable television channels in the United States: CNN, Fox News, and MSNBC. The theoretical framework builds upon Hill's (2008) discussion of the ‘folk theory of race and racism’ in contrast to critical race theory, and asks, to what extent does the mainstream media's discourse about race remain embedded in folk ideas and to what extent (if at all) does the conversation move beyond those ideas? The paper aims to unpack the ideologies of race and language that underpin talk about race and racism in an effort to expose the hidden assumptions in the discourse that hinder more productive dialogue on the topic. (Critical race theory, folk theory of race and racism, George Zimmerman, ideology, language ideology, media discourse, race, race talk, racism, slurs, Trayvon Martin)*
This article focuses on the two national internment programs developed in the United States during World War I from the vantage point of Portland, Oregon, and argues that they unfolded locally. Both the male enemy aliens at risk of internment and the girls and women who experienced confinement due to sexual activity tended to be poor. Authorities deemed that they were, or were likely to become, radicals or prostitutes—but that they were not to be prosecuted as such. Officials could banish or track them more easily as threats to the war effort, rather than as threats to urban social stability and economic development. Scholars of the home front have ignored the evolution of local-federal partnerships to track or intern these two groups and have so far failed to establish how local perceptions of the dangerous poor shaped cooperation with wartime federal authority.
Focused 30keV gallium ion beam, single-pixel drilling combined with backside particle detection is used to fabricate pores having exit diameters as small as ~11 nm in 200 nm-thick silicon nitride membranes. The backside channelplate detector response obtained about the onset of breakthrough is interpreted by plan-view transmission electron microscopy investigations of hole morphology. Immediately prior to breakthrough, there is a rise in detector signal as the local membrane thickness is reduced. This likely occurs as a result of ion transmission and, possibly, forward sputtering. At the dose required for breakthrough a maximum detector signal is obtained thus providing a potential method for end point detection. The focused ion drilling technique avoids broad area beam exposure methods that are often used to reduce hole diameter to nanometer dimension. In addition, the current approach overcomes difficulties in determining a required dose for breakthrough such as those that arise from an inhomogeneous membrane thickness, redeposition, or ion channeling.
This chapter reviews and contrasts the clinical and neuropsychological features of transient global amnesia (TGA) and transient epileptic amnesia (TEA). It discusses their pathophysiology and outlines an approach to the management of patients with transient amnesia. Attacks of TGA follow a wide variety of stresses, including strenuous exertion, sexual intercourse, immersion in water, pain and emotive events. Neuropsychological testing during attacks has confirmed that amnesia is much the most striking deficit in TGA, and is often the only impairment. In principle, the patchy impairment of remote memory which affects a high proportion of patients with TEA must have one of three explanations: failure to encode episodes into long-term memory, failure to consolidate and maintain such memories or failure to retrieve memories which have been successfully stored. TGA is a common and clearly defined clinical syndrome the aetiology of which remains obscure. Patients with TEA often benefit from anti-convulsant medication.
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