Background: Duchenne muscular dystrophy (DMD) is caused by DMD gene mutations. Delandistrogene moxeparvovec is an investigational gene transfer therapy, developed to address the underlying cause of DMD. We report findings from Part 1 (52 weeks) of the two-part EMBARK trial (NCT05096221). Methods: Key inclusion criteria: Ambulatory patients aged ≥4-<8 years with a confirmed DMD mutation within exons 18–79 (inclusive); North Star Ambulatory Assessment (NSAA) score >16 and <29 at screening. Eligible patients were randomized 1:1 to intravenous delandistrogene moxeparvovec (1.33×1014 vg/kg) or placebo. The primary endpoint was change from baseline in NSAA total score to Week 52. Results: At Week 52 (n=125), the primary endpoint did not reach statistical significance, although there was a nominal difference in change from baseline in NSAA total score in the delandistrogene moxeparvovec (2.6, n=63) versus placebo groups (1.9, n=61). Key secondary endpoints (time to rise, micro-dystrophin expression, 10-meter walk/run) demonstrated treatment benefit in both age groups (4-5 and 6-7 years; p<0.05).There were no new safety signals, reinforcing the favorable and manageable safety profile observed to date. Conclusions: Based on the totality of functional assessments including the timed function tests, treatment with delandistrogene moxeparvovec indicates beneficial modification of disease trajectory.

OBJECTIVES/GOALS: Negative emotions (NE) play a pivotal role in addiction-related processes, including tobacco lapse during a quit attempt. Some NEs (e.g., shame, guilt) are posited to lead to a spiraling effect, whereby lapse predicts increased NEs leading to further lapse. This study goal is to examine associations between NEs and lapse. METHODS/STUDY POPULATION: This study examined associations between tobacco lapse and 13 distinct NEs among people who use tobacco and are trying to quit in two tobacco cessation studies. In Study 1, 220 adult (ages 18-74) cigarette users who identified as Black (50% female) participated in a 14-day study where ecological momentary assessment (with assessments approximately every 4 hours) was used to assess emotions and lapse in real-time and real-world settings. In Study 2, 288 adult (ages 18-71) cigarette users who were low socioeconomic status (51% White, 14% Black, 10% Hispanic, 49% female) participated in a 14-day study with the same study protocol as Study 1. Between and lagged within-person associations testing links between distinct NEs and lapse were examined with multilevel modeling with logistic links for binary outcomes. RESULTS/ANTICIPATED RESULTS: Results from Study 1 suggested that at the between-person level, disgust (OR =1.22, CI: 1.05, 1.42), nervousness (OR=1.23, CI:1.05,1.43), guilt (OR=1.40, CI: 1.16,1.69), and sadness (OR=1.18, CI:1.02,1.36) were predictive of higher odds of lapse, and at the within-person level, shame (OR=1.23, CI:1.04,1.45) was associated with higher odds of lapse. Results from Study 2 were similar and suggested that at the between-person level, disgust (OR=1.35, CI: 1.16, 1.56) and guilt (OR=1.88, CI:1.07,3.30), and at the within-person level, shame (OR =1.31, CI:1.10,1.55), were associated with higher odds of lapse. DISCUSSION/SIGNIFICANCE: The present study uses real-time, real-world data to demonstrate the role of distinct NEs on momentary tobacco lapse and helps elucidate specific NE that hinder the ability to abstain from tobacco use during a quit attempt. Results suggest that disgust, guilt, and shame play consistent roles in predicting lapse among diverse samples of tobacco users.

Cooperation among militant organizations contributes to capability but also presents security risks. This is particularly the case when organizations face substantial repression from the state. As a consequence, for cooperation to emerge and persist when it is most valuable, militant groups must have means of committing to cooperation even when the incentives to defect are high. We posit that shared ideology plays this role by providing community monitoring, authority structures, trust, and transnational networks. We test this theory using new, expansive, time-series data on relationships between militant organizations from 1950 to 2016, which we introduce here. We find that when groups share an ideology, and especially a religion, they are more likely to sustain material cooperation in the face of state repression. These findings contextualize and expand upon research demonstrating that connections between violent nonstate actors strongly shape their tactical and strategic behavior.

The discovery of the first electromagnetic counterpart to a gravitational wave signal has generated follow-up observations by over 50 facilities world-wide, ushering in the new era of multi-messenger astronomy. In this paper, we present follow-up observations of the gravitational wave event GW170817 and its electromagnetic counterpart SSS17a/DLT17ck (IAU label AT2017gfo) by 14 Australian telescopes and partner observatories as part of Australian-based and Australian-led research programs. We report early- to late-time multi-wavelength observations, including optical imaging and spectroscopy, mid-infrared imaging, radio imaging, and searches for fast radio bursts. Our optical spectra reveal that the transient source emission cooled from approximately 6 400 K to 2 100 K over a 7-d period and produced no significant optical emission lines. The spectral profiles, cooling rate, and photometric light curves are consistent with the expected outburst and subsequent processes of a binary neutron star merger. Star formation in the host galaxy probably ceased at least a Gyr ago, although there is evidence for a galaxy merger. Binary pulsars with short (100 Myr) decay times are therefore unlikely progenitors, but pulsars like PSR B1534+12 with its 2.7 Gyr coalescence time could produce such a merger. The displacement (~2.2 kpc) of the binary star system from the centre of the main galaxy is not unusual for stars in the host galaxy or stars originating in the merging galaxy, and therefore any constraints on the kick velocity imparted to the progenitor are poor.

Extensive silicified faunules of Middle and Late Ordovician sphinctozoan sponges have been assembled from the northern part of the eastern Klamath Mountains in northern California. The sponges are from eugeosynclinal rocks that are the westernmost Middle Ordovician to Late Devonian rocks at that latitude in North America. Seventeen new species occur in the assemblages, including 10 porate and 7 aporate forms. New genera of porate forms are Amblysiphonelloides and Corymbospongia, and new porate species include: Amblysiphonella grossa, Amblysiphonelloides tubula, A. reticulata, Imperatoria mega, I. media, I. minima, I. irregularis, Corymbospongia adnata, C. mica, and C.(?) perforata. These are the first reported occurrences of Amblysiphonella and Imperatoria in the Ordovician. New aporate genera are Cystothalamiella and Porefieldia, and new aporate species include: Cystothalamiella ducta, C. craticula, C. tuboides, Porefieldia robusta, Girtyocoelia epiporata, and G. canna. This is the oldest known occurrence of Girtyocoelia, which is generally considered a Late Paleozoic form. Cliefdenella obconica n. sp. is characterized by an obconical growth form in contrast to other species of the genus that are more massive or explanate. Cliefdenella is considered here as an imperforate sphinctozoan.

Minor isolated hexactines and hexactine-derived spicules of Hexactinellida were associated with the sphinctozoans. No particular taxa within the class can be distinguished from these individual elements.

The shoo fly Complex of Late Devonian and older Paleozoic age is a regionally extensive rock assemblage in the northern Sierra Nevada of northern California. It consists chiefly of a coherent unit of phyllite, quartzose sandstone, and chert, and a melange unit (Hannah and Moores, 1986). Several limestone lenses in the Taylorsville area comprise the Montgomery Limestone (Diller, 1892, 1908; McMath, 1958; Figure 1). The Montgomery was long considered to be Silurian, largely on the basis of corals, brachiopods, and cephalopods (Diller, 1892, 1908; McMath, 1958; Berry and Boucot, 1970; Merriam, 1972). However, recent analyses of the biota indicate an Ashgill (middle Maysvillian–Gamachian) age (Boucot and Potter, 1977; Harris, personal commun. cited in Hannah and Moores, 1986, p. 790; Potter et al., 1990b; present study).

Cocks and Rong (1989) subdivided the species of the Ordovician brachiopod genus Bimuria into two groups, those with a simple, or not undercut, cardinal process and those with an undercut cardinal process. They placed the second group in a new genus, Cooperea, and grouped Cooperea with Craspedelia in the new subfamily Craspedeliinae of the family Sowerbyellidae. However, the cardinal process of Bimuria ranges from not undercut to undercut within at least two species. Cooperea is therefore placed in synonymy with Bimuria, and Craspedelia is returned to the Bimuriidae.

Cocks and Rong (1989) inferred that, with rare exceptions, the cardinal process of the Plectambonitacea evolved from simple to trifid to undercut. The evidence from Bimuria indicates, however, that the undercut process developed more than once and, in Bimuria, directly from the simple type.

As an adjunct to the above discussion three new species are described: Bimuria gilbertella, Craspedelia intonsa, and Craspedelia sp. 1. Craspedelia is a rarely reported genus documented here for the first time in western North America (northern California). Additional evidence is introduced that the ratio Lmusbv/Lebv decreased from middle to late Ordovician species of Bimuria.

The genus Bimuria Ulrich and Cooper, 1942, is a biogeographically important member of middle and late Ordovician brachiopod faunas of the Callahan–Gazelle area in the eastern Klamath Mountains of northern California. In middle Ordovician deposits the genus is widespread and ranges from Nevada in the west to southwestern Siberia in the east; however, in late Ordovician beds it was previously known only in Sweden and Northern Ireland. In addition to the northern California occurrence reported here, new late Ordovician occurrences are also noted in east-central Alaska–Yukon Territory and Northwest Territories. Three species of Bimuria are described, including the new species, B. californiensis.

A preliminary survey of species described in the literature suggests that the ratio of the length of the dorsal adductor field to the length of the elongate area in the brachial valve decreases from middle to late Ordovician species, and thus may be of biostratigraphic value.

Rousseauspira teicherti, a new genus and species of an unusual, untwisted, horn-shaped gastropod operculum from shallow-subtidal limestones of the Upper Ordovician of Alaska and the Middle Ordovician of California, is described and compared to two other Ordovician opercula, Ceratopea Ulrich, 1911, and Teiichispira Yochelson and Jones, 1968. The shell to which the operculum belonged is not yet known.

We present a brief description of the Berkeley Visible Imaging Tube (BVIT) detector system, which is a user instrument on the 10-m Southern African Large Telescope (SALT), and include some preliminary observational results gained in mid-2011. The data show that BVIT is capable of revealing emission features occurring on time-scales of < 0.1 sec, thus opening up for the general user a window of high time-resolution astronomy at visible wavelengths.

Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010.

Inactivated vaccines prepared from influenza virus strains obtained by the recombination of A/PR/8/34 (H1N1) or A/FM/1/47 (H1N1) viruses with A/Victoria/3/75 (H3N2) virus, were tested for their antigenicity in hamsters. The parental origin of the genes of each cloned recombinant virus was determined by polyacrylamide gel electrophoresis, and vaccines prepared from each strain by concentration, purification on sucrose density gradients and inactivation with formalin. All the recombinant strains used in these studies possessed surface haemagglutinin and neuraminidase antigens derived from the A/Victoria/75 parent strain.

On inoculation into hamsters, at equivalent concentrations, these vaccines varied in their ability to induce haemagglutination-inhibiting (HI) antibodies in the serum. This variation was not dependent on concentration and was observed using neutralization and single radial haemolysis, as well as HI. The possible reasons for the findings are discussed.

Three different types of bivalent influenza virus vaccine, a whole virus, an aqueous-surface-antigen vaccine and an adsorbed-surface-antigen vaccine were tested at three dosage levels in volunteers primed with respect to only one of the haemagglutinin antigens present in the vaccines.

The local and systemic reactions to all three vaccine types were mild in nature and, following first immunization, the aqueous-surface-antigen vaccine was the least reactogenic. The serum haemagglutination-inhibiting antibody response to the A/Victoria/75 component of the vaccines, to which the volunteer population was primed, was greatest following immunization with the aqueous-surface-antigen vaccine; the greatest antibody response to the A/New Jersey/76 component of the vaccines was observed following immunization with whole virus vaccine.

In mid-nineteenth century America, the Arctic adventures of Elisha Kent Kane were a common and central subject for the emerging mass media. Kane's exploits were retold through illustrated newspapers, magazines, and books, but his narratives found one of their widest audiences through the medium of the ‘panorama.’ Initially presented in fixed locations, these panoramas later traveled across the country, combining large moving canvasses with a variety of visual and theatrical effects. Kane's two Arctic expeditions were among the most popular subjects represented by panoramas in the period before the American Civil War. This article examines the history of the panorama as it reflected and shaped public interest in the Arctic regions, including earlier polar expeditions, and gives a detailed account of the Kane panoramas. Other optical media that represented Kane's exploits are also considered. Because of its broad audience and widespread appeal, the panorama, along with other emergent visual technologies, played a vital yet overlooked part, both in disseminating Kane's accomplishments and in elevating Kane to prominence and fame in the mid-nineteenth century.