Journal of Agricultural Science, 47

M. L. Ryder. The pre-natal development of follicle population in the Romney lamb (Vol. 47, Pt. 1)

M. L. Ryder. Observations of nutritional and seasonal changes in the fleeces of some Masham sheep (Vol. 47, Pt. 2)

Will readers please note that the plates illustrating the above articles have been incorrectly placed. The present Plate 7 facing p. 144 should be Plate 1 facing p. 10 and the present Plate 1 facing p. 10 should be Plate 7 facing p. 144.

1. An analysis has been made of milk records from 13,000 cows bred by artificial insemination in Denmark.

2. The herds were divided into three equal groups on the basis of their average production. The variance of yield within herds increased as the average yield increased, but the coefficient of variation declined slightly. The genetic variance was more than five times as large in the high-yielding herds than in the low, and correspondingly the heritabilities in low, medium and high herds were 0·05, 0·15 and 0·22 respectively. These were estimated from the variation observed between progeny groups of the same 152 bulls at each production level.

3. No evidence was obtained of any sire-herd interaction for yield, either within or between management levels. The true ranking of bulls for breeding value was apparently the same at all levels.

4. The heritability of fat content in the three groups was 0·27, 0·47 and 0·49 respectively, and no evidence of sire-herd interaction was found.

5. The contemporary comparison method of assessing A.I. bulls for yield was found to have the accuracy expected in theory.

6. These results are discussed in relation to those of other workers with which there are some discrepancies. On our results, a policy of choosing bulls on the basis of their daughters' performance in high-yielding herds should be the most satisfactory way of progeny-testing bulls used in artificial insemination.

1. A comparison has been made between progeny tests in special stations and those available from field records. The variation between sires in milk yield is much greater at the stations even when they are compared with herds at the same production level.

2. In respect of yield the repeatability of station tests in the field is not good. The regression of field results on station test is only about 0·2 in herds at all production levels. For fat percentage, the regression is 0·5.

3. The tests in special stations (averaging seventeen daughters per bull) were compared with those in high herds (average twenty-seven daughters) in their accuracy of prediction of the performance of bulls in the poorer herds. The field test was slightly (but not significantly) better for both milk yield and fat content.

4. In milk yield, there is undoubtedly some extra variation between progeny groups at the stations which is not repeated in the field. Perhaps the tying of the heifers in distinct daughter groups in the byre may be a cause of this. However, even without this, it is doubted whether the test stations can give as much useful information on the numerical aspects of performance as the field records usually available. It is suggested that the splitting of daughter groups within the byre might increase the accuracy of the special station in the assessment of yield.

1. The results are presented of an investigation in which the growth and development of forty single male sheep foetuses was examined. The material, obtained from slaughterhouses, included seventeen specimens of known age, while further details of the remainder were lacking. For these reasons, and the fact that the specimens were preserved in 10% formalin prior to dissection, relative, rather than absolute, trends were discussed. This had been achieved by expressing all values as percentages of those obtained from four single male lambs dissected at birth.

2. In order to age the experimental material with the greatest possible accuracy, data were collected from the literature on foetal weights and crownrump lengths of specimens of known age. Mean values were computed and normographs established for the pre-natal period between 18 days and fullterm. By employing the method of least squares, it was not possible to fit single curves to the data; in the case of both weight and crown-rump length the curves had to be divided into five segments in order to establish the relationships with age. Apart from a rather marked change in the rate of growth and development between the 109th and 110th days of gestation, the apparent ‘breaks’ in the curves were not considered important. It would seem that lack of data at certain stages, together with the heterogeneity of the population studied, were greatly responsible for the differential rates of growth and development recorded.

3. The data were inadequate to examine fully the effect of fixation on foetal weight; however, it would appear that whereas specimens weighing less than 200 g. and over 1200 g. lost weight during the course of preservation, foetuses intermediate between the above values gained weight. Since the causes could not be satisfactorily explained, the need for further investigation was emphasized.

4. Of the foetal measurements recorded, head length and, particularly, head width were shown to be earlier maturing than chest circumference and chest depth, while crown-rump length made even greater proportional increases during pre-natal life. In general, the results supported accepted theory that the retardation of rate of development proceeds in an anterior-posterior direction prior to birth.

5. By dissecting the head from the body and weighing each unit separately, it was shown that the head decreases in relative size throughout foetal life. Head:body ratio, however, was found to be unsuitable as a criterion of pre-natal age, since foetuses of equal age may differ appreciably in this respect. It was shown, for example, that a lamb at birth, though above the average in terms of absolute weight, may possess the conformation of a 120-day-old foetus on the basis of head: body ratio.

6. Dissections of the major constituent parts of the foetal body indicated the skinned head to be the earliest maturing, followed in order by the organs (weighed en masse), dressed carcass, total skin and, finally, the skinned feet and tail, which were latest maturing. The order in which these parts grow before birth appeared explicable primarily upon a basis of functional necessity.

7. The skull was shown to develop to a relatively lesser degree during foetal life than the mandibles, while skull measurements indicated the cranial portion to be earlier maturing than the facial. Linear measurements of the various vertebral regions at different stages of development did not yield entirely satisfactory results, but it was evident that the anterior cervical vertebrae are earlier maturing than those of the posterior extremity (caudal vertebrae).

8. Weights of the major units of both thoracic and pelvic limbs indicated the former to be slightly earlier maturing. In contrast to the direction of retardation of growth during post-natal life, in the foetus the gradient followed a proximo-distal direction; scapula and pelvis, for example, being earlier maturing than metacarpus and metatarsus.

9. Linear development of individual bones was shown to be earlier maturing than growth in weight. However, on the basis of length measurements, the course of development proceeded in a similar direction as the trends established in terms of weight, i.e. proximo-distally. Width of pelvis, on the contrary, was shown to be earlier maturing than width of scapula; this was explained in terms, particularly, of earlier developing cartilage in the former bone.

10. In terms of weight, m. longissimus dorsi appeared to be earlier maturing than m. rectus femoris and m. gastrocnemius lateralis; between the latter two muscles no marked difference in rate of growth could be established. The results indicated that these three muscles develop at much the same rate in respect of length and depth during pre-natal life, but m. longissimus dorsi showed relatively less development in width over the same period of time than the other muscles.

11. Of each of the above-mentioned muscles, the cross-diameter of fifty individual fibres was measured by means of an ocular micrometer. Again, a tendency was observed for fibres of m. longissimus dorsi to be earlier maturing than those of m. rectus femoris or m. gastrocnemius.

12. Development of muscle-fibre diameter during foetal life was examined further on the overall mean for the three muscles studied, i.e. the mean of 150 measurements per foetus. The results showed that whereas muscle-fibre diameter increased but slightly (approximately 19·5%) during the first two-thirds of pre-natal life, the increase thereafter was substantial; on the basis of a linear regression, the increase amounted to 113·2% from about 108 days to full-term. The data thus appears to support the accepted view that muscular growth occurs initially by an increase in the number of fibres, and in late foetal life primarily by hypertrophy.

13. The dispersion of muscle-fibre sizes, in absolute measure, was shown to increase with advancing foetal age, the range at birth being considerable. In accord with the results of other investigators, a small proportion of small fibres was observed between approximate ages of 61 and 68 days which, apparently, were not present at either earlier or later stages.

14. Measurement of fibres from muscles situated in different anatomical regions of the body indicated that the muscles of the head were earlier maturing than those of the trunk, and muscles of the thoracic limb earlier maturing than those of the pelvic limb. In agreement with the results obtained on bone weights and measurements, the retardation appeared to proceed in a proximo-distal direction, particularly in the case of the pelvic limb.

1. The effect of a short, unchanging day-length of 6 hr. on age at sexual maturity and egg production has been observed.

2. The birds matured at the same age as the controls but at a lower body weight.

3. Egg production over 58 weeks was considerably reduced.

4. An increase in day-length between December and August always increased production.

5. A decrease in day-length between February and August always decreased production; this decrease was greater the later in the year that the change occurred.

6. It is concluded that age at sexual maturity is not affected by the absolute length of the day, and that egg production is affected both by the absolute length and by the change in length.

1. Growth and feed conversion efficiency of pigs between weaning and 100 lb. live weight were improved by adding a vitamin B12 a supplement to simplified all-plant rations containing, principally, barley and groundnut meal. This response was obtained with less than one-third of the U.S. recommended allowance of vitamin B12 in the rations of growing pigs.

2. No such response was obtained when a vitamin B12 supplement was added to rations containing milling by-products and grass meal.

3. When pigs were fed to a scale based on live weight, those that received an all-plant ration (containing milling by-products and grass meal, and relying on groundnut meal as the main source of supplementary protein to give a total content of 18·4 to 18·8% crude protein) gave 90–93% as good growth as pigs that were fed rations of similar total digestible nutrient content containing 14·5–15·5% crude protein and 3·6 or 7·0% white fish meal. Calculations suggest that such groundnut meal rations may be slightly deficient in lysine, but probably not deficient in tryptophan or ‘cystine + methionine’.

4. When pigs were fed to appetite, those that received rations containing 3·6% white fish meal and no milling by-products or grass meal, ate significantly more than those that had either ‘milling by-products + grass meal’ or no fish meal in their rations; they grew proportionately faster, with no improvement in the efficiency of feed conversion.

5. The carcasses of pigs that had received all-plant rations from weaning to bacon weight were of good quality, and their measurements did not differ from those of pigs that had received fish meal to 100 lb. live weight.

1. An investigation is reported in which the relationship was examined between body size and muscle fibre size of nineteen newborn lambs and including twelve males and seven females. Body size was measured in terms of the dead body weight and that of the dressed carcass, while muscle fibre size was estimated on the basis of the mean crossdiameter of 150 fibres per lamb.

2. It was shown that the dressing (or carcass) percentage tends to increase with an increase in body weight, from 42·26% at a body weight of 2000 g. to 49·04% at 7000 g.

3. Of the 2850 cross-diameters recorded, the majority (33·8%) of the fibres measured between 8·0 and 9·6 μ, while individual fibres varied in size from 1·6 to 22·4 μ.

4. Highly significant, positive correlations were shown to exist between both body (r = 0·996) and carcass (r = 0·946) weight, and mean muscle fibre diameter, indicating that differences in size between the lambs may be accounted for largely by corresponding variations in the size of individual muscle fibres.

5. Of the three muscles sampled m. gastrocnemius had the largest mean fibre diameter (10·38μ), followed in decreasing order by m. rectus femoris (9·72 μ) and m. longissimus dorsi (9·09 μ). These inter-muscle differences were significant at the 1% level of probability.

6. Ram lambs had significantly thicker muscle fibres (10·32 μ) than ewe lambs (8·72 μ), but also weighed the heavier and produced heavier dressed carcasses. Some evidence was produced, however, in support of the theory that at comparable weights males possess thinner individual, and therefore a greater number of fibres than females.

1. Two digestibility trials were carried out to determine the s.e. and t.d.n. of swedes, fodder beet and grass silage when fed alone and when each kind of root was fed with silage at separate feeds. In addition, fodder beet and silage were fed together at the same feed.

2. The results were examined to determine if the feeding of roots and silage together in the diet, but at separate feeds, had led to any complementary effect.

3. No complementary effect was found when swedes and silage were fed at separate feeds.

4. A negative complementary effect was found when fodder beet and silage were fed at separate feeds.

5. The s.e. and t.d.n. values of the fodder beet plus silage at each feed were significantly higher than when the two foods were given at separate feed times.

6. The s.e. and t.d.n. of the swede dry matter were higher than the standard figures published. An explanation is given.

7. The nutritive value of the fodder-beet dry matter was not higher than that of swede dry matter.

8. The results of the digestibility trials are compared with some calculated results from a cattle feeding trial.

The yields of cotton from five three-course rotations are given; they show marked seasonal variations, the variations of the different rotations being in close accord with one another. The average yields of the rotations are related fairly closely to the average soil nitrates; in individual years this is not so, though considerable responses to nitrogenous manuring are always obtained on Gezira soil.

The distribution of nitrate in Gezira soil shows an unusual accumulation at depth; though cotton roots can reach the zone of accumulation, the variability of nitrate at these depths renders it impossible to relate changes to uptake. The figures show that under growing cotton the whole of the upper 4 ft. of soil can have low nitrates (of the order of 1 p.p.m.). Unless the roots obtain nitrate from the fifth and sixth feet accumulations the plants must be taking up nitrate as fast as it is formed, and the nitrate-supplying power of the soil must be of first importance. Figures are given showing differences in nitrification in soil from the different rotations.

The effect of heavy nitrogenous manuring on soil nitrate is shown to be largely confined to the year of application, and no accumulations could be demonstrated. The average recovery of added nitrogen in this experiment is only of the order of 10%. The yields of the heavily manured treatments show the same violent fluctuations as do the unmanured treatments.

In the three-course rotation experiment surface nitrates in August and September in the wider rotations are strongly negatively correlated with the rainfall of the period 1 July to 15 August. Over the period July to September the distribution of nitrates in the upper 2 ft. of the profile changes; there are differences between the rotations in this respect.

1. The digestibility of samples of heather of 3, 7 and 14 years of age has been determined.

2. The experimental technique was the same as that used in the previous digestibility studies of heather.

3. The digestibility of the organic matter, and of all its components other than crude fibre and ether extractives, was found to decline with advancing age. This decline became apparent in heather of 7 years of age.

4. Gross digestible energy values were determined for the three ages of heather under investigation. Starch equivalents derived from these values proved to be lower than starch equivalents calculated in the usual way.

5. Confirmation of earlier conclusions concerning the value of heather as a source of energy has been obtained.