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Post-cooling survival, growth and deformity rates in zebrafish embryos (Danio rerio)

Published online by Cambridge University Press:  18 December 2017

Maria do Carmo Faria Paes  and
Laura Satiko Okada Nakaghi
Maria do Carmo Faria Paes*
Affiliation:
Department of Animal Morphology and Physiology, São Paulo State University, Campus Jaboticabal, São Paulo, Brazil. Access way Professor Paulo Donato Castellane, S/N, Jaboticabal-SP, CEP 14884–900, Brazil.
Laura Satiko Okada Nakaghi
Affiliation:
Aquaculture Centre of São Paulo State University (CAUNESP), Jaboticabal, São Paulo, Brazil.
*
All correspondence to: Maria do Carmo Faria Paes. Department of Animal Morphology and Physiology, São Paulo State University, Campus Jaboticabal, São Paulo, Brazil. Access way Professor Paulo Donato Castellane, S/N, Jaboticabal-SP, CEP 14884–900, Brazil. Tel:/Fax: +55 16 3209 2654 (ext. 232). E-mail: maria.paes@fcav.unesp.br
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Summary

This study investigated and analysed survival, growth and macro- and microscopic damage during the development of zebrafish embryos up to the adult stage after undergoing cooling. The embryos at 50% epiboly stage were selected, submerged in cryoprotectant solution of methanol and sucrose, cooled gradually to 0 ± 2°C temperature, and divided into two groups with different storage times (6 and 18 h). Subsequently, the embryos were reheated, rehydrated and incubated normally. The experiment lasted 5 months and, from hatching onward, the larvae were examined, collected and processed at pre-established time intervals. The hatching rate was significantly higher for the larvae stored for 18 h compared with the 6-h group. However, embryos from this group gave rise to a larger number of malformations, and these were much more severe compared with those in the 6 h group, which led to a higher mortality in the long term. Regarding larval length, the animals of the 6 h group had higher mean total length compared with the 18 h group, but both treatments were inferior to the control. Numerous macro- and microscopic malformations were observed and, in both treatments, only the morphologically normal individuals were able to develop to the adult stage, with organ development similar to the control, except for the gonads that were still undifferentiated in treated animals.

Keywords

Fish embryosLow temperatureMalformationMicroscopyMorphology
Type
Research Article
Information
Zygote , Volume 26 , Issue 1 , February 2018 , pp. 76 - 88
Copyright
Copyright © Cambridge University Press 2017 

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