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Pentoxifylline treatment had no detrimental effect on sperm DNA integrity and clinical characteristics in cases with non-obstructive azoospermia

Published online by Cambridge University Press:  19 October 2022

Maryam Mahaldashtian Open the ORCID record for Maryam Mahaldashtian [Opens in a new window] ,
Mohammad Ali Khalili Open the ORCID record for Mohammad Ali Khalili [Opens in a new window] ,
Esmat Mangoli ,
Saeed Zavereh  and
Fatemeh Anbari
Maryam Mahaldashtian
Affiliation:
Department of Reproductive Biology, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Mohammad Ali Khalili*
Affiliation:
Department of Reproductive Biology, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Esmat Mangoli
Affiliation:
Department of Reproductive Biology, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Saeed Zavereh
Affiliation:
School of Biology, Damghan University, Damghan, Iran
Fatemeh Anbari
Affiliation:
Department of Reproductive Biology, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
*
Author for correspondence: Mohammad Ali Khalili. Professor of Embryology. Department of Reproductive Biology, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Tel: +98 3518247085. Fax: +98 3518247087. E-mail: khalili59@hotmail.com
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Summary

The aim of this study was to assess the consequences of treatment with pentoxifylline (PTX), an inducer of sperm motility, on sperm DNA fragmentation (SDF) and clinical characteristics in non-obstructive azoospermia (NOA) patients. The pilot study included 15 NOA patients. Half of each sperm sample before and after rapid freezing, was treated with PTX (3.6 mM /l, 30 min) as the PTX group and the remaining samples were considered as the control. SDF and sperm motility were assessed in each group. The clinical study comprised 30 fresh testicular sperm extractions (TESE) and 22 post-thawed TESE intracytoplasmic sperm injection cycles. Half of the mature oocytes from each patient were injected with PTX-treated spermatozoa and the remaining oocytes were injected with non-treated spermatozoa. Fertilization was assessed at 16 h post injection. Embryo transfer was carried out on day 2 after fertilization. Chemical pregnancy was assessed 2 weeks after transfer. PTX was found to significantly increase (P < 0.05) sperm motility. There was an insignificant difference in SDF rates between the groups (P > 0.05). In patient ovaries given fresh TESE, there was not any significant difference in clinical characteristics (P > 0.05). In patient ovaries given post-thawed TESE, there was a significant difference in the number of 2PN and in embryo formation (P < 0.05). Differences in the results of chemical pregnancy were insignificant (P > 0.05) between the groups. In addition, there was not any correlation between DNA fragmentation index and sperm motility and laboratory outcomes. Therefore, obtaining viable spermatozoa using PTX was more effective in post-thawed TESE regime patients in terms of 2PN and in embryo formation, deprived of damaging effects on sperm DNA integrity.

Keywords

Chemical pregnancyCryopreservationDNA fragmentationNon-obstructive azoospermiaPentoxifylline
Type
Research Article
Information
Zygote , Volume 31 , Issue 1 , February 2023 , pp. 8 - 13
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Copyright
© The Author(s), 2022. Published by Cambridge University Press

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