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Occurrence of chromosomal aneuploidy in rabbit oocytes and embryos at different developmental stages

Published online by Cambridge University Press:  26 November 2009

Jozef Curlej ,
Jozef Bulla  and
Peter Chrenek
Jozef Curlej*
Affiliation:
Animal Production Research Centre Nitra, Hlohovecka 2, 951 41 Luzianky, Slovak Republic Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic.
Jozef Bulla
Affiliation:
Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic.
Peter Chrenek
Affiliation:
Department of Genetics and Animal Reproduction, Animal Production Research Centre, Nitra, Slovak Republic. Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic.
*
All correspondence to: Jozef Curlej. Animal Production Research Centre Nitra, Hlohovecka 2, 951 41 Luzianky, Slovak Republic; Tel: +421 37 654 6236. Fax: +421 37 654 6189. e-mail: jojonr@zoznam.sk
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Summary

The aim of our study was to analyse chromosomal aneuploidy occurence in rabbit oocytes and embryos. Chromosomal analysis was done in rabbit oocytes and rabbit preimplantation embryos at 2-, 4- and 8-cell stages derived from in vivo fertilization. For mitotic cycle synchronization at the metaphase stage, 2-, 4- and 8-cell embryos were incubated in k-DMEM medium, supplemented with colcemid (1 μg/ml), for 7, 12 or 13h respectively. Success of metaphase synchronization was at values of 100, 86.1 and 92.2% for 2-, 4- and 8-cell embryos respectively. Recovery rate of analysable metaphase plates was reached at 58.8%, 83.9% and 59.8% for 2-, 4- and 8-cell embryos and 100% for oocytes. Significant difference (p < 0.01) in aneuploidy rate between oocytes (40.7%) and 2-cell embryos (62.5%) was found. These results demonstrate higher efficiency of synchronization of embryo cells at the metaphase stage, what may contribute to elevating the proportion of analysable nuclei.

Keywords

AneuploidyChromosomeEmbryoRabbitSynchronization
Type
Research Article
Information
Zygote , Volume 18 , Issue 3 , August 2010 , pp. 203 - 207
Copyright
Copyright © Cambridge University Press 2009

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