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Morphogenesis-related gene-expression profile in porcine oocytes before and after in vitro maturation

Published online by Cambridge University Press:  03 July 2017

Joanna Budna ,
Adrian Chachuła ,
Dominika Kaźmierczak ,
Marta Rybska ,
Sylwia Ciesiółka ,
Artur Bryja ,
Wiesława Kranc ,
Sylwia Borys ,
Agnieszka Żok  and
Dorota Bukowska
...Show all authors
Joanna Budna
Affiliation:
Department of Histology and Embryology, Department of Anatomy, Poznań University of Medical Sciences, 6 Święcickiego St., 60–781 Poznań, Poland.
Adrian Chachuła
Affiliation:
Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland
Dominika Kaźmierczak
Affiliation:
Institute of Veterinary Sciences, Poznań University of Life Sciences, Poznań, Poland
Marta Rybska
Affiliation:
Institute of Veterinary Sciences, Poznań University of Life Sciences, Poznań, Poland
Sylwia Ciesiółka
Affiliation:
Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland
Artur Bryja
Affiliation:
Department of Anatomy, Poznań University of Medical Sciences, Poznań, Poland
Wiesława Kranc
Affiliation:
Department of Anatomy, Poznań University of Medical Sciences, Poznań, Poland
Sylwia Borys
Affiliation:
Department of Anatomy, Poznań University of Medical Sciences, Poznań, Poland
Agnieszka Żok
Affiliation:
Department of Social Sciences, Poznań University of Medical Sciences, Poznań, Poland
Dorota Bukowska
Affiliation:
Institute of Veterinary Sciences, Poznań University of Life Sciences, Poznań, Poland
Paweł Antosik
Affiliation:
Institute of Veterinary Sciences, Poznań University of Life Sciences, Poznań, Poland
Małgorzata Bruska
Affiliation:
Department of Anatomy, Poznań University of Medical Sciences, Poznań, Poland
Klaus P. Brüssow
Affiliation:
Institute of Veterinary Sciences, Poznań University of Life Sciences, Poznań, Poland
Michał Nowicki
Affiliation:
Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland
Maciej Zabel
Affiliation:
Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland
Bartosz Kempisty*
Affiliation:
Department of Histology and Embryology, Department of Anatomy, Poznań University of Medical Sciences, 6 Święcickiego St., 60–781 Poznań, Poland. Department of Histology and Embryology, Poznań University of Medical Sciences, Poznań, Poland Department of Anatomy, Poznań University of Medical Sciences, Poznań, Poland
*
All correspondence to: Bartosz Kempisty. Department of Histology and Embryology, Department of Anatomy, Poznań University of Medical Sciences, 6 Święcickiego St., 60–781 Poznań, Poland. Tel:. +48 61 8546418. Fax:+48 61 8546440, E-mail: bkempisty@ump.edu.pl
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Summary

Mammalian oocyte maturation is achieved when oocytes reach metaphase II (MII) stage, and accumulate mRNA and proteins in the cytoplasm following fertilization. It has been shown that oocytes investigated before and after in vitro maturation (IVM) differ significantly in transcriptomic and proteomic profiles. Additionally, folliculogenesis and oogenesis is accompanied by morphogenetic changes, which significantly influence further zygote formation and embryo growth. This study aimed to determine new transcriptomic markers of porcine oocyte morphogenesis that are associated with cell maturation competence. An Affymetrix microarray assay was performed on an RNA template isolated from porcine oocytes before (n = 150) and after (n = 150) IVM. The brilliant cresyl blue (BCB) staining test was used for identification of cells with the highest developmental capacity. DAVID (Database for Annotation, Visualization, and Integrated Discovery) software was used for the extraction of the genes belonging to a cell morphogenesis Gene Ontology group. The control group consisted of freshly isolated oocytes. In total, 12,000 different transcripts were analysed, from which 379 genes were downregulated and 40 were upregulated in oocytes following IVM. We found five genes, SOX9, MAP1B, DAB2, FN1, and CXCL12, that were significantly upregulated in oocytes after IVM (in vitro group) compared with oocytes analysed before IVM (in vivo group). In conclusion, we found new transcriptomic markers of oocyte morphogenesis, which may be also recognized as significant mediators of cellular maturation capacity in pigs. Genes SOX9, MAP1B, DAB2, FN1, and CXCL12 may be involved in the regulation of the MII stage oocyte formation and several other processes that are crucial for porcine reproductive competence.

Keywords

In vitro maturationMicroarrayOocytesPig
Type
Research Article
Information
Zygote , Volume 25 , Issue 3 , June 2017 , pp. 331 - 340
Copyright
Copyright © Cambridge University Press 2017 

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Footnotes

7

Both authors contributed equally to this work

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