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Histochemical and morphological features of biopsied and stripped oocytes from the Brazilian endangered teleost pirapitinga, Brycon nattereri (Characiformes)

Published online by Cambridge University Press:  15 January 2014

Alexandre N. Maria ,
Laura H. Orfão ,
Elizete Rizzo ,
Alexandre Ninhaus-Silveira  and
Ana T.M. Viveiros
Alexandre N. Maria
Affiliation:
Department of Animal Science (DZO), Federal University of Lavras, UFLA, Lavras, MG, Brazil.
Laura H. Orfão
Affiliation:
Department of Animal Science (DZO), Federal University of Lavras, UFLA, Lavras, MG, Brazil.
Elizete Rizzo
Affiliation:
Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, UFMG, Belo Horizonte, MG, Brazil.
Alexandre Ninhaus-Silveira
Affiliation:
Department of Biology and Animal Science, São Paulo State University - FEIS, Ilha Solteira, SP, Brazil.
Ana T.M. Viveiros*
Affiliation:
Department of Animal Science (DZO), Federal University of Lavras, UFLA, PO Box 3037, Lavras, MG, 37200–000, Brazil. Department of Animal Science (DZO), Federal University of Lavras, UFLA, Lavras, MG, Brazil.
*
All correspondence to: Ana TM Viveiros. Department of Animal Science (DZO), Federal University of Lavras, UFLA, PO Box 3037, Lavras, MG, 37200–000, Brazil. e-mail: ana.viveiros@dzo.ufla.br; anatmviveiros@hotmail.com
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Summary

The aim of the present study was to characterize biopsied and stripped oocytes from the Brazilian endangered teleost pirapitinga (Brycon nattereri) using histochemical and morphological analyses. Biopsied oocytes had a mean diameter of 2.225 mm (modal diameter: 2.312 mm), complete vitellogenesis and a central or slightly eccentric nucleus. Neutral polysaccharides were detected in the follicular cells, zona radiata and yolk globules, while acidic polysaccharides were detected in the follicular cells and cortical alveoli. Ten out of the 19 females treated with two doses of carp pituitary extract (cPE) released oocytes, which were also analysed. Stripping occurred 292 ± 39 degree-hours after the second dose of cPE and led to a mean spawning weight of 36.2 g, 10% spawning index, 241 oocytes/g of ova, 8222 oocytes/female and 23 oocytes/g of body weight. Stripped oocytes had a mean diameter of 2.33 mm and a mode at 2.375 mm, were weakly adhesive and coloration ranged from wine to brown. Under scanning electron microscopy, stripped oocytes exhibited a single funnel-shaped micropyle located at the animal pole and a zona radiata that measured 7.7 μm in thickness with eight pore canals/μm2. Oocyte morphology in Brycon nattereri is similar to that found in other species of the genus, except for the larger size and weaker adhesiveness. These findings provide essential information for a better understanding of the reproductive biology of B. nattereri and the establishment of conservation measures for this threatened species.

Keywords

FishOocyte surfaceReproductionSpawningUltrastructure
Type
Research Article
Information
Zygote , Volume 23 , Issue 3 , June 2015 , pp. 360 - 366
Copyright
Copyright © Cambridge University Press 2014 

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