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Folliculogenesis-related genes are differently expressed in secondary and tertiary ovarian follicles

Published online by Cambridge University Press:  22 April 2021

L.B.S. Moura ,
D.M. Magalhães-Padilha Open the ORCID record for D.M. Magalhães-Padilha [Opens in a new window] ,
A.N.P. Morais ,
F.L.N. Aguiar Open the ORCID record for F.L.N. Aguiar [Opens in a new window] ,
J. Geisler–Lee ,
A. Wischral ,
M.O. Gastal ,
G.R. Fonseca ,
M. Geisler  and
J.R. Figueiredo
L.B.S. Moura
Affiliation:
Postgraduate Biotechnology, Potiguar University, Natal, RN, Brazil
D.M. Magalhães-Padilha*
Affiliation:
Postgraduate Biotechnology, Potiguar University, Natal, RN, Brazil
A.N.P. Morais
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, UECE, Brazil
F.L.N. Aguiar
Affiliation:
Department of Veterinary Medicine, Sousa Campus, IFPB, PB, Brazil
J. Geisler–Lee
Affiliation:
Department of Plant Biology, SIU, Carbondale, IL, USA
A. Wischral
Affiliation:
Department of Animal Science, Food and Nutrition, SIU, Carbondale, IL, USA
M.O. Gastal
Affiliation:
Department of Animal Science, Food and Nutrition, SIU, Carbondale, IL, USA
G.R. Fonseca
Affiliation:
Department of Animal Science, Food and Nutrition, SIU, Carbondale, IL, USA
M. Geisler
Affiliation:
Department of Plant Biology, SIU, Carbondale, IL, USA
J.R. Figueiredo
Affiliation:
Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, UECE, Brazil
*
Correspondence: D.M. Magalhães-Padilha. Postgraduate Biotechnology, Potiguar University, Natal, RN, Brazil. E-mail: dmmvet@hotmail.com
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Summary

The relative mRNA abundance of 10 genes associated with folliculogenesis was compared between late preantral (secondary) and early antral (tertiary) ovarian follicles in goats. In total, 100 follicles in each category were mechanically isolated. The relative transcript abundance of the mRNAs were determined by qPCR. Data were analyzed using unpaired Student’s t-test. Of the 10 tested genes, ABLIM mRNA was not detected in either follicle category, six genes (SLIT3, TYMS, GTPBP1, AKR1C4, PIK3R6, and MAOB) were upregulated in secondary follicles compared with tertiary follicles, and three genes (ARHGEF12, CLEC6A, and CYTL1) showed similar mRNA abundances in both secondary and tertiary follicles. In conclusion, SLIT3, GTPBP1, AKR1C4, and PIK3R6 mRNA abundance was upregulated in secondary follicles (preantral phase) compared with in tertiary follicles (antral phase) in goats.

Keywords

Gene expressionGoatsPCRSecondary folliclesTertiary folliclesTranscriptional profiles
Type
Short Communication
Information
Zygote , Volume 29 , Issue 6 , December 2021 , pp. 503 - 506
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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