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Expression of genes associated with fertility in the uterus and oviduct of heifers challenged with lipopolysaccharide

Published online by Cambridge University Press:  12 January 2022

Giuliana A. Ferronato ,
Joao A. Alvarado-Rincón Open the ORCID record for Joao A. Alvarado-Rincón [Opens in a new window] ,
Andressa S. Maffi ,
Antônio A. Barbosa ,
Bernardo G. Gasperin ,
Augusto Schneider ,
Rafael G. Mondadori Open the ORCID record for Rafael G. Mondadori [Opens in a new window] ,
Cássio C. Brauner  and
Marcio N. Corrêa
Giuliana A. Ferronato
Affiliation:
Facultad de Ciencias Agropecuarias, Universidad de la Salle, Campus Utopia, Yopal - Casanare, 850008, Colombia
Joao A. Alvarado-Rincón
Affiliation:
Federal University of Pelotas, Pelotas, RS 96160-000, Brazil
Andressa S. Maffi
Affiliation:
Federal University of Pelotas, Pelotas, RS 96160-000, Brazil
Antônio A. Barbosa
Affiliation:
Federal University of Pelotas, Pelotas, RS 96160-000, Brazil
Bernardo G. Gasperin
Affiliation:
Federal University of Pelotas, Pelotas, RS 96160-000, Brazil
Augusto Schneider
Affiliation:
Federal University of Pelotas, Pelotas, RS 96160-000, Brazil
Rafael G. Mondadori
Affiliation:
Federal University of Pelotas, Pelotas, RS 96160-000, Brazil
Cássio C. Brauner
Affiliation:
Federal University of Pelotas, Pelotas, RS 96160-000, Brazil
Marcio N. Corrêa*
Affiliation:
Federal University of Pelotas, Pelotas, RS 96160-000, Brazil
*
Author for correspondence: Marcio Nunes Corrêa. Federal University of Pelotas, Pelotas, RS96160–000, Brazil. E-mail: marcio.nunescorrea@gmail.com
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Summary

Lipopolysaccharide (LPS) endotoxemia has been negatively associated with fertility. This study aimed to investigate the effect of LPS-induced inflammation on gene expression associated with bovine fertility in the uterus and oviduct. Sixteen healthy heifers were divided into two groups. The LPS group (n = 8) received two intravenous (i.v.) injections of 0.5 µg/kg of body weight of LPS with a 24-h interval, and the control group (n = 8) received two i.v. injections of saline solution with the same interval of time. All the animals had the follicular wave synchronized. Three days after the second injection of LPS, all animals were slaughtered and uterine and oviduct samples were collected. Gene expression associated with inflammatory response, thermal and oxidative stresses, oviduct environment quality, and uterine environment quality was evaluated. Body temperature and leucogram demonstrated that LPS induced an acute systemic inflammatory response. In the uterus, the expression of PTGS2 and NANOG genes was downregulated by the LPS challenge. However, no change in expression was observed in the other evaluated genes in the uterus, nor those evaluated in the oviduct. In conclusion, the inflammatory process triggered by LPS did not persist in the uterus and oviduct 3 days after challenge with LPS. Nonetheless, reduction in PTGS2 and NANOG expression in the uterus suggested that, indirectly, LPS may have a prolonged effect, which may affect corpus luteum and endometrial functions.

Keywords

CattleEndotoxemiaLPSInflammationReproduction
Type
Short Communication
Information
Zygote , Volume 30 , Issue 4 , August 2022 , pp. 584 - 587
Copyright
© The Author(s), 2022. Published by Cambridge University Press

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References

Alvarado-Rincón, JA, Gindri, PC, Mion, B, Giuliana de Ávila, F, Barbosa, AA, Maffi, AS, Pradieé, J, Mondadori, RG, Corrêa, MN, Ligia Margareth Cantarelli, P and Schneider, A (2019). Early embryonic development of bovine oocytes challenged with LPS in vitro or in vivo. Reproduction 158, 453–63.CrossRefGoogle ScholarPubMed
Bidne, KL, Dickson, MJ, Ross, JW, Baumgard, LH and Keating, AF (2018). Disruption of female reproductive function by endotoxins. Reproduction 155, R169–81.CrossRefGoogle ScholarPubMed
Cronin, JG, Turner, ML, Goetze, L, Bryant, CE and Sheldon, IM (2012). Toll-like receptor 4 and MYD88-dependent signaling mechanisms of the innate immune system are essential for the response to lipopolysaccharide by epithelial and stromal cells of the bovine endometrium. Biol Reprod 86, 51.10.1095/biolreprod.111.092718CrossRefGoogle ScholarPubMed
Ibrahim, S, Salilew-Wondim, D, Rings, F, Hoelker, M, Neuhoff, C, Tholen, E, Looft, C, Schellander, K and Tesfaye, D (2015). Expression pattern of inflammatory response genes and their regulatory microRNAs in bovine oviductal cells in response to lipopolysaccharide: Implication for early embryonic development. PLoS One 10, e0119388.CrossRefGoogle ScholarPubMed
Jaenisch, R and Young, R (2008). Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming. Cell 132, 567–82.CrossRefGoogle ScholarPubMed
Parent, J and Fortier, MA (2005). Expression and contribution of three different isoforms of prostaglandin E synthase in the bovine endometrium. Biol Reprod 73, 36–44.CrossRefGoogle ScholarPubMed
Stefanska, B, Człapa, W, Pruszynska-Oszmałek, E, Szczepankiewicz, D, Fievez, V, Komisarek, J, Stajek, K and Nowak, W (2018). Subacute ruminal acidosis affects fermentation and endotoxin concentration in the rumen and relative expression of the CD14/TLR4/MD2 genes involved in lipopolysaccharide systemic immune response in dairy cows. J Dairy Sci 101, 1297–310.CrossRefGoogle ScholarPubMed
Swangchan-uthai, T, Lavender, CR, Cheng, Z, Fouladi-Nashta, AA and Wathes, DC (2012). Time course of defense mechanisms in bovine endometrium in response to lipopolysaccharide. Biol Reprod 87, 135.CrossRefGoogle ScholarPubMed
Waldron, MR, Nishida, T, Nonnecke, BJ and Overton, TR (2003). Effect of lipopolysaccharide on indices of peripheral and hepatic metabolism in lactating cows. J Dairy Sci 86, 3447–59.CrossRefGoogle ScholarPubMed
Yagi, Y, Shiono, H, Shibahara, T, Chikayama, Y, Nakamura, I and Ohnuma, A (2002). Increase in apoptotic polymorphonuclear neutrophils in peripheral blood after intramammary infusion of Escherichia coli lipopolysaccharide. Vet Immunol Immunopathol 89, 115–25.CrossRefGoogle ScholarPubMed
Zebeli, Q, Sivaraman, S, Dunn, SM and Ametaj, BN (2013). Intermittently induced endotoxaemia has no effect on post-challenge plasma metabolites, but increases body temperature and cortisol concentrations in periparturient dairy cows. Res Vet Sci 95, 1155–62.CrossRefGoogle ScholarPubMed
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