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Expression of enzymes involved in the synthesis of prostaglandin E2 in bovine in vitro-produced embryos

Published online by Cambridge University Press:  14 January 2011

Marie Saint-Dizier ,
Bénédicte Grimard ,
Catherine Guyader-Joly ,
Patrice Humblot  and
Andrew A. Ponter
Marie Saint-Dizier*
Affiliation:
AgroParisTech, UFR Génétique Elevage Reproduction, 16 rue Claude Bernard, F-75231 Paris, France. AgroParisTech, UFR Génétique Elevage Reproduction, 16 rue Claude Bernard, F-75231 Paris, France. ENVA, UMR 1198 Biologie du développement et reproduction, F-94704 Maisons Alfort, France. INRA, UMR 1198 Biologie du développement et reproduction, F-78350 Jouy en Josas, France.
Bénédicte Grimard
Affiliation:
ENVA, UMR 1198 Biologie du développement et reproduction, F-94704 Maisons Alfort, France. INRA, UMR 1198 Biologie du développement et reproduction, F-78350 Jouy en Josas, France. Ecole Nationale Vétérinaire d'Alfort, 7 avenue du Général de Gaulle, F-94704 Maisons Alfort, France.
Catherine Guyader-Joly
Affiliation:
UNCEIA, Département Recherche et Développement, 13 rue Jouët, F-94704 Maisons Alfort, France. Station R&D unceia/UCEAR, F-38300 Chateauvillain, France.
Patrice Humblot
Affiliation:
UNCEIA, Département Recherche et Développement, 13 rue Jouët, F-94704 Maisons Alfort, France.
Andrew A. Ponter
Affiliation:
ENVA, UMR 1198 Biologie du développement et reproduction, F-94704 Maisons Alfort, France. INRA, UMR 1198 Biologie du développement et reproduction, F-78350 Jouy en Josas, France. Ecole Nationale Vétérinaire d'Alfort, 7 avenue du Général de Gaulle, F-94704 Maisons Alfort, France.
*
All correspondence to: Marie Saint-Dizier. AgroParisTech, UFR Génétique Elevage Reproduction, 16 rue Claude Bernard, F-75231 Paris, France. Tel: +33 1 44 08 18 52. Fax: +33 1 44 08 86 22. e-mail: stdizier@agroparistech.fr
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Summary

Prostaglandin E2 (PGE2) may play a major role in embryo development and the establishment of pregnancy in cattle. The biosynthesis of PGE2 implies the sequential transformation of arachidonic acid to PGH2 by cyclooxygenases (COXs), then the conversion of PGH2 to PGE2 by prostaglandin E synthases (PGESs). Quantitative RT-PCR was used to examine the expression of COX-1, COX-2, microsomal PGES-1 (mPGES-1), microsomal PGES-2 (mPGES-2) and cytosolic PGES (cPGES) mRNAs in day 7 in vitro-produced (IVP) embryos from oocytes collected by ovum pick-up in Holstein heifers. Transcripts for COX-2 and mPGES-1 were detected in all embryos, whereas transcripts for COX-1 and mPGES-2 were not detected and cPGESs were at the limit of detection in 40% of embryos. Levels of COX-2 and mPGES-1 mRNAs were significantly higher in blastocysts and expanded blastocysts than in morulae and early blastocysts. Furthermore, excellent-quality embryos (grade 1) displayed higher levels of both COX-2 and mPGES-1 than did embryos of good and medium qualities (grades 2–3). Our results suggest that bovine IVP embryos at the morula and blastocyst stages use exclusively the COX-2/mPGES-1 pathway for PGE2 biosynthesis, and that PGE2 is potentially involved in blastocyst expansion and developmental competence.

Keywords

bovineembryoCOXprostaglandin E2synthases
Type
Research Article
Information
Zygote , Volume 19 , Issue 3 , August 2011 , pp. 277 - 283
Copyright
Copyright © Cambridge University Press 2011

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