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Effects of melatonin on production of reactive oxygen species and developmental competence of bovine oocytes exposed to heat shock and oxidative stress during in vitro maturation

Published online by Cambridge University Press:  07 June 2019

Fernanda de Castro Cavallari ,
Cláudia Lima Verde Leal ,
Roth Zvi  and
Peter J. Hansen Open the ORCID record for Peter J. Hansen [Opens in a new window]
Fernanda de Castro Cavallari
Affiliation:
Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga 13635-900, Brazil Department of Animal Sciences, D. H. Barron Reproductive and Perinatal Biology Research Program and Genetics Institute, University of Florida, Gainesville, Florida 32611-0910, USA
Cláudia Lima Verde Leal
Affiliation:
Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga 13635-900, Brazil
Roth Zvi
Affiliation:
Department of Animal Sciences, Robert H. Smith Faculty of Agricultural, Food & Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel
Peter J. Hansen*
Affiliation:
Department of Animal Sciences, D. H. Barron Reproductive and Perinatal Biology Research Program and Genetics Institute, University of Florida, Gainesville, Florida 32611-0910, USA
*
*Address for correspondence: Peter J. Hansen. Department of Animal Sciences, D. H. Barron Reproductive and Perinatal Biology Research Program and Genetics Institute, University of Florida, Gainesville, Florida 32611-0910, USA. E-mail Hansen@animal.ufl.edu
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Summary

Heat shock may disrupt oocyte function by increasing the generation of reactive oxygen species (ROS). We evaluated the capacity of the antioxidant melatonin to protect oocytes using two models of oxidative stress – heat shock and the pro-oxidant menadione. Bovine cumulus–oocyte complexes (COC) were exposed in the presence or absence of 1 µM melatonin to the following treatments during maturation: 38.5°C, 41°C and 38.5°C+5 µM menadione. In the first experiment, COC were matured for 3 h with 5 µM CellROX® and analyzed by epifluorescence microscopy to quantify production of ROS. The intensity of ROS was greater for oocytes exposed to heat shock and menadione than for control oocytes. Melatonin reduced ROS intensity for heat-shocked oocytes and oocytes exposed to menadione, but not for control oocytes. In the second experiment, COC were matured for 22 h. After maturation, oocytes were fertilized and the embryos cultured for 7.5 days. The proportion of oocytes that cleaved after fertilization was lower for oocytes exposed to heat shock and menadione than for control oocytes. Melatonin increased cleavage for heat-shocked oocytes and oocytes exposed to menadione, but not for control oocytes. Melatonin tended to increase the developmental competence of embryos from heat-shocked oocytes but not for embryos from oocytes exposed to menadione or from control oocytes. In conclusion, melatonin reduced production of ROS of maturing oocytes and protected oocytes from deleterious effects of both stresses on competence of the oocyte to cleave after coincubation with sperm. These results suggest that excessive production of ROS compromises oocyte function.

Keywords

EmbryoHeat shockMelatoninMenadioneReactive oxygen species.
Type
Research Article
Information
Zygote , Volume 27 , Issue 3 , June 2019 , pp. 180 - 186
Copyright
© Cambridge University Press 2019 

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