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Effect of zinc and copper on preimplantation mouse embryo development in vitro and metallothionein levels

Published online by Cambridge University Press:  26 September 2008

Francesca Vidal  and
Juan Hidalgo
Francesca Vidal
Affiliation:
Autonomous University of Barcelona, Barcelona, Spain
Juan Hidalgo*
Affiliation:
Autonomous University of Barcelona, Barcelona, Spain
*
Juan Hidalgo, Departamento de Biologia Celular y Fisiologia, Unidad de Fisiologia Animal, Facultad de Ciencias, Universidad Autonoma de Barcelona, Bellaterra 08193, Barcelona, Spain. Fax: (3) 581 22 95.
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Extract

The effect of zinc and copper on the in vitro development of mouse preimplantation embryos and on metallothionein (MT) levels was studied by exposing the embryos to 100 μM concentrations of the metals for 24 h at the 1-cell,2-cell, 6-8-cell, morula and blastocyst stages. Zinc affected embryo development in the early but not in the late stages, whereas copper affected it more generally. The combined presence of both metals caused a stronger embryotoxicity. MT levels were measured by radioimmunoassay and were found to be similar at all developmental stages, though possibly higher at the blastocyst stage. The exposure of embryos to zinc and copper increased MT levels significantly only at the blastocyst stage, supporting previously published results on MT mRNA levels.

Keywords

CopperEmbryoMetallothioneinZinc
Type
Review Article
Information
Zygote , Volume 1 , Issue 3 , August 1993 , pp. 225 - 229
Copyright
Copyright © Cambridge University Press 1993

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