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Culture of preantral ovarian follicles of Bos taurus indicus with alpha-lipoic acid

Published online by Cambridge University Press:  25 August 2021

Larissa Zamparone Bergamo Open the ORCID record for Larissa Zamparone Bergamo [Opens in a new window] ,
Denis Vinicius Bonato ,
Camila Bizarro-Silva Open the ORCID record for Camila Bizarro-Silva [Opens in a new window] ,
Francieli Gesleine Capote Bonato ,
Suellen Miguez González ,
Ana Carolina Rossaneis ,
Waldiceu A. Verri Jr ,
Fábio Morotti  and
Marcelo Marcondes Seneda
Larissa Zamparone Bergamo*
Affiliation:
Laboratory of Biotechnology of Animal Reproduction, Department of Veterinary Clinics, Center of Agrarian Sciences, State University of Londrina, Londrina, Parana, Brazil Department of Veterinary Science, Federal University of Parana, Palotina, Parana, Brazil
Denis Vinicius Bonato
Affiliation:
Laboratory of Biotechnology of Animal Reproduction, Department of Veterinary Clinics, Center of Agrarian Sciences, State University of Londrina, Londrina, Parana, Brazil Paranaense University, Umuarama, Parana, Brazil
Camila Bizarro-Silva
Affiliation:
Laboratory of Biotechnology of Animal Reproduction, Department of Veterinary Clinics, Center of Agrarian Sciences, State University of Londrina, Londrina, Parana, Brazil Animal Reproduction Laboratory, School of Life Sciences, Pontifical Catholic University of Parana, Toledo, Parana, Brazil
Francieli Gesleine Capote Bonato
Affiliation:
Laboratory of Biotechnology of Animal Reproduction, Department of Veterinary Clinics, Center of Agrarian Sciences, State University of Londrina, Londrina, Parana, Brazil
Suellen Miguez González
Affiliation:
Laboratory of Biotechnology of Animal Reproduction, Department of Veterinary Clinics, Center of Agrarian Sciences, State University of Londrina, Londrina, Parana, Brazil
Ana Carolina Rossaneis
Affiliation:
Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Londrina, Parana, Brazil
Waldiceu A. Verri Jr
Affiliation:
Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Londrina, Parana, Brazil
Fábio Morotti
Affiliation:
Laboratory of Biotechnology of Animal Reproduction, Department of Veterinary Clinics, Center of Agrarian Sciences, State University of Londrina, Londrina, Parana, Brazil
Marcelo Marcondes Seneda
Affiliation:
Laboratory of Biotechnology of Animal Reproduction, Department of Veterinary Clinics, Center of Agrarian Sciences, State University of Londrina, Londrina, Parana, Brazil
*
Author for correspondence: Larissa Zamparone Bergamo. Laboratory of Biotechnology of Animal Reproduction, 86057-970, Londrina, Parana, Brazil. E-mail: larissabergamo1@hotmail.com
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Summary

The aim of this study was to evaluate follicular development, morphological integrity, and antioxidant potential of preantral ovarian follicles from Bos taurus indicus females grown in vitro with alpha-lipoic acid. Ovaries (n = 24) of Bos taurus indicus (n = 12) females were collected during slaughter and fragmented. A randomly obtained fragment from each pair of ovaries was fixed in Bouin (non-cultivated control; D0). These fragments were intended for classical histology (morphology and evaluation of follicular growth), and a fragment from each pair of ovaries was frozen at −80°C (non-cultivated control; D0), and assigned for analysis of oxidative stress [thiobarbituric acid reactive substances (TBARS), nitroblue tetrazolium (NBT), and ferric reducing antioxidant power (FRAP)]. The remaining fragments were cultured in vitro for 6 (D6) or 12 (D12) days, containing only minimum essential medium (MEM) or MEM supplemented with alpha-lipoic acid (50, 100, or 250 ng/ml), on an extracellular matrix of agarose gel, in an oven at 38.5ºC. Every 2 days, 100% of the culture medium was replaced. Supplementation with 100 ng/ml was effective for maintaining follicular integrity after 6 days of culture (primordial: 51.28%; development: 36.88%; P < 0.0001). There was no difference (P > 0.05) between treatments compared with the non-cultivated control treatment (D0), using the NBT and TBARS assays. Therefore, supplementation of the in vitro culture medium of bovine preantral ovarian follicles with a concentration of 100 ng/ml of alpha-lipoic acid at 6 days of culture was effective for maintaining follicular integrity and, after 6 days, maintaining stable levels of reactive oxygen species.

Keywords

AntioxidantsBovineIn vitro culture of preantral folliclesNeloreROS
Type
Research Article
Information
Zygote , Volume 30 , Issue 2 , April 2022 , pp. 206 - 212
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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