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CFAP43-mediated intra-manchette transport is required for sperm head shaping and flagella formation

Published online by Cambridge University Press:  13 October 2020

Yi Yu Open the ORCID record for Yi Yu [Opens in a new window] ,
Jiaxiong Wang ,
Liming Zhou ,
Haibo Li ,
Bo Zheng  and
Shenmin Yang Open the ORCID record for Shenmin Yang [Opens in a new window]
Yi Yu
Affiliation:
Center for Reproduction and Genetics, Suzhou Municipal Hospital, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, 215002, China Reproduction Center, Key Laboratory of Birth Defects Prevention, Ningbo Women and Children’s Hospital, Ningbo, 315012, China
Jiaxiong Wang
Affiliation:
Center for Reproduction and Genetics, Suzhou Municipal Hospital, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, 215002, China
Liming Zhou
Affiliation:
Reproduction Center, Key Laboratory of Birth Defects Prevention, Ningbo Women and Children’s Hospital, Ningbo, 315012, China
Haibo Li
Affiliation:
Reproduction Center, Key Laboratory of Birth Defects Prevention, Ningbo Women and Children’s Hospital, Ningbo, 315012, China
Bo Zheng*
Affiliation:
Center for Reproduction and Genetics, Suzhou Municipal Hospital, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, 215002, China State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 211166, China
Shenmin Yang*
Affiliation:
Center for Reproduction and Genetics, Suzhou Municipal Hospital, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, 215002, China State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 211166, China
*
Authors for correspondence: Shenmin Yang; Bo Zheng. Center for Reproduction and Genetics, Suzhou Municipal Hospital, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou City, Jiangsu Province, 215002, China. Tel:/Fax: +86 15895902765. E-mail: drim2004@126.com; mansnoopy@163.com
Authors for correspondence: Shenmin Yang; Bo Zheng. Center for Reproduction and Genetics, Suzhou Municipal Hospital, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou City, Jiangsu Province, 215002, China. Tel:/Fax: +86 15895902765. E-mail: drim2004@126.com; mansnoopy@163.com
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Summary

Mutation in CFAP43 leads to severe asthenozoospermia and multiple morphological abnormalities of the sperm flagellum (MMAF) in both human and mouse. Previous studies have shown that disruption of intra-manchette transport (IMT) caused failure of flagellum assembly and sperm head shaping. In a previous study, therefore, we postulated that disruption of IMT may contribute to the failure of sperm flagellum formation and result in MMAF, however the mechanisms underlying these defects are still poorly understood. Cfap43-deficient mice were studied here to reveal the cellular mechanisms of abnormal sperm head morphology and MMAF. Depletion of Cfap43 led to abnormal spermiogenesis and caused MMAF, sperm head abnormality and oligozoospermia. Furthermore, both abnormal manchette and disorganized ectoplasmic specialization (ES) could be observed at the elongated spermatids in Cfap43-deficient mice. Therefore, our findings demonstrated that, in mice, CFAP43-mediated IMT is essential for sperm head shaping and sperm flagellum formation.

Keywords

Cfap43Flagella formationIMTManchetteMMAF
Type
Research Article
Information
Zygote , Volume 29 , Issue 1 , February 2021 , pp. 75 - 81
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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