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Birefringence parameter available for quantitative analysis of human zona hardness

Published online by Cambridge University Press:  27 July 2010

Hiroshi Iwayama ,
Shinichi Hochi  and
Masanori Yamashita
Hiroshi Iwayama*
Affiliation:
Yamashita Ladies’ Clinic, Kobe, Hyogo 651–0086, Japan.
Shinichi Hochi
Affiliation:
Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan.
Masanori Yamashita
Affiliation:
Yamashita Ladies’ Clinic, Kobe, Hyogo 651–0086, Japan.
*
All correspondence to: Hiroshi Iwayama. Yamashita Ladies’ Clinic, Kobe, Hyogo 651–0086, Japan. Tel: +81 78 265 6475. Fax: +81 78 265 6476. e-mail: hiwayama@hotmal.co.jp
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Summary

This study was designed to investigate whether a non-invasive birefringence parameter, determined using the Oosight™ imaging system, is useful for estimating the hardness of human zona pellucida (ZP). The value for retardance (R) × thickness (T), but not R or T alone, of ZP was positively correlated (r = 0.92, p < 0.0001) with its hardness estimated by the time required for a 0.1% protease solution to solubilize ZP at 37 °C. In a model experiment to induce ZP puncture by Fluorinert™ fluid microinjection (sham-hatching), the R × T value at the punctured site was positively correlated (r = 0.78, p < 0.01) with the hardness of the ZP as estimated by the maximum expansion rate. The R × T values of ZP in in vitro fertilization-derived embryos (21.6 ± 7.5) and intracytoplasmic sperm injection-derived embryos (20.8 ± 6.3) were significantly higher than that in unfertilized metaphase II oocytes (16.6 ± 6.1; p < 0.05). The R × T value after in vitro hatching of viable blastocysts (10.8 ± 6.2) was significantly lower than that of unexpanded morulae and early blastocysts (19.0 ± 4.0; p < 0.05), while the value of expanding blastocysts (15.3 ± 4.1) was intermediate. In conclusion, hardness of human ZP can be estimated non-invasively by birefringence-based microscopic observation.

Keywords

BirefringenceHatchingHuman ARTRetardanceZona hardness
Type
Research Article
Information
Zygote , Volume 19 , Issue 4 , November 2011 , pp. 323 - 329
Copyright
Copyright © Cambridge University Press 2010

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References

Biggers, J.D. & Racowsky, C. (2002). The development of fertilized human ova to the blastocyst stage in KSOMAA medium: is a two-step protocol necessary? Reprod. Biomed. Online 5, 133–40.CrossRefGoogle Scholar
Cohen, J., Elsner, C., Kort, H., Malter, H., Massey, J., Mayer, M.P. & Wiemer, K. (1990). Impairment of the hatching process following IVF in the human and improvement of implantation by assisting hatching using micromanipulation. Hum. Reprod. 5, 7–13.CrossRefGoogle ScholarPubMed
Cohen, J., Alikani, M., Trowbridge, J. & Rosenwaks, Z. (1992). Implantation enhancement by selective assisted hatching using zona drilling of human embryos with poor prognosis. Hum. Reprod. 7, 685–91.Google Scholar
Drobnis, E.Z., Andrew, J.B. & Katz, D.F. (1988). Biophysical properties of the zona pellucida measured by capillary suction: is zona hardening a mechanical phenomenon? J. Exp. Zool. 245, 206–19.Google Scholar
Gook, D., Martic, M., Borg, J. & Edgar, D.H. (2004). Identification of zona pellucida proteins during human folliculogenesis. Hum. Reprod. 19 (Suppl. 1), i140.Google Scholar
Gonzales, D.S. & Bavister, B.D. (1995). Zona pellucida escape by hamster blastocysts in vitro is delayed and morphologically different compared with zona escape in vivo. Biol. Reprod. 52, 470–80.CrossRefGoogle ScholarPubMed
Gu, Y.F., Lu, C.F., Lin, G. & Lu, G.X. (2010). A comparative analysis of the zona pellucida birefringence of fresh and frozen–thawed human embryos. Reproduction 139, 121–7.Google Scholar
Herrler, A. & Beier, H.M. (2000). Early embryonic coats: morphology, function, practical applications. An overview. Cells Tissues Organs 166, 233–46.CrossRefGoogle ScholarPubMed
Khalilian, M., Navidbakhsh, M., Valojerdi, M.R., Chizari, M. & Yazdi, P.E. (2010). Estimating Young's modulus of zona pellucida by micropipette aspiration in combination with theoretical models of ovum. J. R. Soc. Interface 7, 687–94.CrossRefGoogle ScholarPubMed
Kilani, S.S., Cooke, S., Kan, A.K. & Chapman, M.G. (2006). Do age and extended culture affect the architecture of the zona pellucida of human oocytes and embryos? Zygote 14, 39–44.CrossRefGoogle ScholarPubMed
Larman, M.G., Sheehan, C.B. & Gardner, D.K. (2006). Calcium-free vitrification reduces cryoprotectant-induced zona pellucida hardening and increases fertilization rates in mouse oocytes. Reproduction 131, 53–61.Google Scholar
Lefièvre, L., Conner, S.J., Salpekar, A., Olufowobi, O., Ashton, P., Pavlovic, B., Lenton, W., Afnan, M., Brewis, I.A., Monk, M., Hughes, D.C. & Barrat, C.L. (2004). Four zona pellucida glycoproteins are expressed in the human. Hum. Reprod. 19, 1580–6.Google Scholar
Madaschi, C., Aoki, T., de Almeida Ferreira Braga, D.P., de Cássia Sávio Figueira, R., Semião Francisco, L., Iaconelli, A. Jr. & Borges, E. Jr. (2009). Zona pellucida birefringence score and meiotic spindle visualization in relation to embryo development and ICSI outcomes. Reprod. Biomed. Online 18, 681–6.CrossRefGoogle ScholarPubMed
Manna, C., Rienzi, L., Greco, E., Sbracia, M., Rahman, A., Poverini, R., Siracusa, G. & De Felici, M. (2001). Zona pellucida solubility and cortical granule complements in human oocytes following assisted reproductive techniques. Zygote 9, 201–10.CrossRefGoogle ScholarPubMed
Mantoudis, E., Podsiadly, B.T., Gorgy, A., Venkat, G. & Craft, I.L. (2001). A comparison between quarter, partial and total laser assisted hatching in selected infertility patients. Hum. Reprod. 16, 2182–6.Google Scholar
Matson, P.L., Graefling, J., Junk, S.M., Yovich, J.L. & Edirisinghe, W.R. (1997). Cryopreservation of oocytes and embryos: use of a mouse model to investigate effects upon zona hardness and formulate treatment strategies in an in-vitro fertilization programme. Hum. Reprod. 12, 1550–3.Google Scholar
Montag, M., Schimming, T., Köster, M., Zhou, C., Dorn, C., Rösing, B., Van Der Ven, H. & Ven der Ven, K. (2008). Oocyte zona birefringence intensity is associated with embryonic implantation potential in ICSI cycles. Reprod. Biomed. Online 16, 239–44.CrossRefGoogle ScholarPubMed
Moos, J., Faundes, D., Kopf, G.S. & Schultz, R.M. (1995). Composition of the human zona pellucida and modifications following fertilization. Hum. Reprod. 10, 2467–71.Google Scholar
Murayama, Y., Mizuno, J., Kamakura, H., Fueta, Y., Nakamura, H., Akaishi, K., Anzai, K., Watanabe, A., Inui, H. & Omata, S. (2006). Mouse zona pellucida dynamically changes its elasticity during oocyte maturation, fertilization and early embryo development. Hum. Cell 19,119–25.CrossRefGoogle ScholarPubMed
Oldenbourg, R. (1996). A new view on polarization microscopy. Nature 381, 811–2.Google Scholar
Pelletier, C., Keefe, D.L. & Trimarchi, J.R. (2004). Noninvasive polarized light microscopy quantitatively distinguishes the multilaminar structure of the zona pellucida of living human eggs and embryos. Fertil. Steril. 81, 850–6.CrossRefGoogle ScholarPubMed
Rama Raju, G.A., Prakash, G.J., Krishna, K.M. & Madan, K. (2007). Meiotic spindle and zona pellucida characteristics as predictors of embryonic development: a preliminary study using PolScope imaging. Reprod. Biomed. Online 14, 166–74.Google Scholar
Schiewe, M.C., Araujo, E. Jr., Asch, R.H. & Balmaceda, J.P. (1995). Enzymatic characterization of zona pellucida hardening in human eggs and embryos. J. Assist. Reprod. Genet. 12, 2–7.Google Scholar
Shen, Y., Stalf, T., Mehnert, C., Eichenlaub-Ritter, U. & Tinneberg, H.R. (2005). High magnitude of light retardation by the zona pellucida is associated with conception cycles. Hum. Reprod. 20, 1596–606.CrossRefGoogle ScholarPubMed
Sun, Y., Wan, K.T., Roberts, K.P., Bischof, J.C. & Nelson, B.J. (2003). Mechanical property characterization of mouse zona pellucida. IEEE Trans. Nanobiosci. 2, 279–86.CrossRefGoogle ScholarPubMed
Tsubamoto, H., Hasegawa, A., Nakata, Y., Naito, S., Yamasaki, N. & Koyama, K. (1995). Expression of recombinant human zona pellucida protein 2 and its binding capacity to spermatozoa. Biol. Reprod. 61, 1649–54.Google Scholar
Vanderzwalmen, P., Bertin, G., Debauche, Ch., Standaert, V., Bollen, N., van Roosendaal, E., Vandervorst, M., Schoysman, R. & Zech, N. (2003). Vitrification of human blastocysts with the hemi-straw carrier: application of assisted hatching after thawing. Hum. Reprod. 18, 1504–11.CrossRefGoogle ScholarPubMed
Wacogne, B., Pieralli, C., Roux, C. & Gharbi, T. (2008). Measuring the mechanical behaviour of human oocytes with a very simple SU-8 micro-tool. Biomed. Microdev. 10, 411–9.Google Scholar
Yanagida, K., Katayose, H., Yazawa, H., Kimura, Y., Konnai, K. & Sato, A. (1999). The usefulness of a piezo-micromanipulator in intracytoplasmic sperm injection in humans. Hum. Reprod. 14, 448–53.Google Scholar