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Antioxidant activities and lipid peroxidation status in human follicular fluid: age-dependent change

Published online by Cambridge University Press:  29 April 2021

H. Debbarh Open the ORCID record for H. Debbarh [Opens in a new window] ,
N. Louanjli ,
S. Aboulmaouahib ,
M. Jamil Open the ORCID record for M. Jamil [Opens in a new window] ,
L. Ahbbas ,
I. Kaarouch ,
O. Sefrioui  and
R. Cadi
H. Debbarh*
Affiliation:
Laboratory of Molecular Genetic Physiopathology and Biotechnology, Department of Biology Ain Chock Faculty of Sciences, Hassan II University, Casablanca, Morocco
N. Louanjli
Affiliation:
Anfa Fertility Center, Private Clinic of In Vitro Fertilization and Endoscopic Surgery, Casablanca, Morocco Labomac IVF Center and Clinical Laboratory Medicine, Casablanca, Morocco
S. Aboulmaouahib
Affiliation:
Anfa Fertility Center, Private Clinic of In Vitro Fertilization and Endoscopic Surgery, Casablanca, Morocco
M. Jamil
Affiliation:
Laboratory of Molecular Genetic Physiopathology and Biotechnology, Department of Biology Ain Chock Faculty of Sciences, Hassan II University, Casablanca, Morocco
L. Ahbbas
Affiliation:
Anfa Fertility Center, Private Clinic of In Vitro Fertilization and Endoscopic Surgery, Casablanca, Morocco
I. Kaarouch
Affiliation:
Anfa Fertility Center, Private Clinic of In Vitro Fertilization and Endoscopic Surgery, Casablanca, Morocco
O. Sefrioui
Affiliation:
Anfa Fertility Center, Private Clinic of In Vitro Fertilization and Endoscopic Surgery, Casablanca, Morocco
R. Cadi
Affiliation:
Laboratory of Molecular Genetic Physiopathology and Biotechnology, Department of Biology Ain Chock Faculty of Sciences, Hassan II University, Casablanca, Morocco
*
Author for correspondence: H. Debbarh. Laboratory of Molecular Genetic Physiopathology and Biotechnology, Department of Biology Ain Chock Faculty of Sciences, Hassan II University, Casablanca, Morocco. E-mail: debbarhhasnae2017@gmail.com
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Summary

Maternal age is a significant factor influencing in vitro fertilization (IVF) outcomes. Oxidative stress (OS) is one of the major causes of age-related cellular and molecular damage. The purpose of this work was to investigate the correlation between maternal age with intrafollicular antioxidants and OS markers in follicular fluid (FF), and also to determine the OS status in patients of advanced age. This study was a prospective study including 201 women undergoing IVF whose age was between 24 and 45 years old. FF samples were obtained from mature follicles at the time of oocyte retrieval. After treatment of FF, lipid peroxidation levels (MDA) and enzyme activities such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and glutathione (GSH) level were evaluated using spectrophotometry. The results indicated that the age cutoff point for increasing the MDA level was fixed at 37 years, allowing the study to be differentiated into two age groups. Group I included patients whose age was less than 37 years, and group II included patients whose age was greater than or equal 37 years. Statistical analysis revealed that MDA and GSH levels and GR activity were significantly higher in group II compared with group I. The SOD and CAT activities were significantly less in group II compared with group I. We concluded that from 37 years old a reproductive ageing was accompanied by a change in the antioxidant pattern in FF that impaired reactive oxygen species scavenging efficiency.

Keywords

Antioxidant enzymesMaternal ageLipid peroxidation
Type
Research Article
Information
Zygote , Volume 29 , Issue 6 , December 2021 , pp. 490 - 494
Copyright
© Ain Chock Faculty of Sciences, Hassan II University, Casablanca, Morocco., 2021. Published by Cambridge University Press

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