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Pronucleus formation, DNA synthesis and metaphase entry in porcine oocytes following intracytoplasmic injection of murine spermatozoa

Published online by Cambridge University Press:  27 November 2003

Bong-Ki Kim
Affiliation:
Department of Animal Sciences, Chungbuk University, Cheongju, Chungbuk, Korea National Livestock Research Institute, RDA, Korea
Sun Hong Cheon
Affiliation:
Department of Animal Sciences, Chungbuk University, Cheongju, Chungbuk, Korea Research Center for Transgenic Cloned Pigs Chungnam National University, Daejeon, Korea
Youn Jeong Lee
Affiliation:
Department of Animal Sciences, Chungbuk University, Cheongju, Chungbuk, Korea
Sun Ho Choi
Affiliation:
National Livestock Research Institute, RDA, Korea
Xiang Shun Cui
Affiliation:
Department of Animal Sciences, Chungbuk University, Cheongju, Chungbuk, Korea
Nam-Hyung Kim
Affiliation:
Department of Animal Sciences, Chungbuk University, Cheongju, Chungbuk, Korea Research Center for Transgenic Cloned Pigs Chungnam National University, Daejeon, Korea Research Center for Bioresource and Health Chungbuk National University, Cheongju, Chungbuk, Korea

Abstract

The onset of pronucleus formation and DNA synthesis in porcine oocytes following the injection of porcine or murine sperm was determined in order to obtain insights into species-specific paternal factors that contribute to fertilisation. Similar frequencies of oocytes with female pronuclei were observed after injection with porcine sperm or with murine sperm. In contrast, male pronuclei formed 8-9 h following the injection of porcine sperm, and 6-8 h following the injection of murine sperm. After pronucleus formation maternally derived microtubules were assembled and appeared to move both male and female pronuclei to the oocyte centre. A few porcine oocytes entered metaphase 22 h after the injection of murine sperm, but normal cell division was not observed. The mean time of onset of S-phase in male pronuclei was 9.7 h following porcine sperm injection and 7.4 h following mouse sperm injection. Ultrastructural observation revealed that male pronuclei derived from murine sperm in porcine oocytes are morphologically similar to normal male pronuclei in porcine zygotes. These results suggest that species-specific paternal factors influence the onset of pronucleus formation and DNA synthesis. However, normal nuclear cytoplasmic interactions were observed in porcine S-phase oocytes following murine sperm injection.

Type
Research Article
Copyright
2003 Cambridge University Press

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Pronucleus formation, DNA synthesis and metaphase entry in porcine oocytes following intracytoplasmic injection of murine spermatozoa
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